Effect of p53 on regeneration of periodontal tissue : Using small molecule compounds

• Project/Area Number: 22792082
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Periodontal dentistry
• Research Institution: Osaka University
• Principal Investigator: KITAGAKI Jirouta 大阪大学, 歯学部附属病院, 特任助教 (90570292)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 骨代謝 / p53 / 低分子化合物 / 再生医学

Research Abstract

In this study, we investigated the effect of p53 on differentiation of periodontal ligament tissue using the small molecule compounds HLI98 and HLI373. HLI98 and HLI373 increased the expression of p53 in osteoblast. However, these compounds enhanced cell death when we performed long-term cell culture because of the toxicity of these compounds. Therefore, we utilized Bortezomib which inhibits degradation of p53 and stabilizes p53 expression. Bortezomib clearly induced p53 expression and calcification of periodontal ligament cell line MPDL-22. These data indicate that p53 enhances differentiation of periodontal ligament cell.

Research Products

• [Journal Article] DNA intercalator korkormicin A preferentially kills transformed cells expressing wild type p53 (2011)
  • Author(s): Kitagaki J and Yang Y
  • Journal Title: Biochemical and Biophysical Research Communications Volume: 414 Pages: 186-191
  • Peer Reviewed
• [Journal Article] Fgf8 is essential for formation of the ductal system in the male reproductive tract (2011)
  • Author(s): Kitagaki J, Ueda Y, Chi X, Sharma N, Elder C, Truffer E, Costantini F, Lewandoski M and Perantoni AO
  • Journal Title: Development Volume: 35 Pages: 5369-78
  • Peer Reviewed
• [Journal Article] A novel Wilms tumor 1(WT1) target gene negatively regulates the WNT signaling pathway (2010)
  • Author(s): Kim MS, Yoon SK, Bollig F, Kitagaki J, Hur W, Whye NJ, Wu YP, Rivera MN, Park JY, Kim HS, Malik K, Bell DW, Englert C, Perantoni AO and Lee SB
  • Journal Title: Journal of Biological Chemistry Volume: 285 Pages: 14585-93
  • Peer Reviewed
• [Presentation] Korkormicin Aはp53依存的にガン細胞のアポトーシスを誘導する (2011)
  • Author(s): 北垣次郎太
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2011-12-13
• [Presentation] SIRT1による歯根膜細胞の石灰化制御 (2011)
  • Author(s): 中村友美、山下元三、河原貴展、橋本悠平、梶川哲宏、森健太、前田憲一郎、北垣次郎太、柳田学、山田聡、北村正博、村上伸也
  • Organizer: 第135回日本歯科保存学会秋季学術大会
  • Place of Presentation: 大阪
  • Year and Date: 2011-10-21
• [Presentation] p53新規誘導化合物の同定とその作用機序の解析 (2011)
  • Author(s): 北垣次郎太、村上伸也
  • Organizer: 第135回日本歯科保存学会秋季学術大会
  • Place of Presentation: 大阪
  • Year and Date: 2011-10-21
• [Presentation] P53誘導新規化合物の同定とその作用機序の解析 (2011)
  • Author(s): 北垣次郎太
  • Organizer: 日本歯科保存学会
  • Place of Presentation: 大阪市(大阪国際交流センター)
  • Year and Date: 2011-10-21
• [Presentation] 歯周病原生細菌P. g感染が血球新生に及ぼす影響 (2011)
  • Author(s): 前田憲一郎、久保田実木子、大原廣之、伊山舜吉、沢田啓吾、竹立匡秀、山下元三、北垣次郎太、市川朋生、北村正博、村上伸也
  • Organizer: 第135回日本歯科保存学会秋季学術大会
  • Place of Presentation: 大阪
  • Year and Date: 2011-10-20
• [Presentation] Fgf8 is essential for male reproductive tract development (2010)
  • Author(s): Jirouta Kitagaki
  • Organizer: 第33回日本分子生物学会年会
  • Place of Presentation: 神戸
  • Year and Date: 2010-12-10
• [Presentation] アポトーシス誘導遺伝子p53に対する選択誘導化合物の同定とその作用機序の解析 (2010)
  • Author(s): 北垣次郎太、村上伸也
  • Organizer: 第53回日本歯周病学会秋季学術大会
  • Place of Presentation: 高松
  • Year and Date: 2010-09-19
• [Presentation] アポトーシス誘導遺伝子p53に対する選択的誘導化合物の同定とその作用機序の解析 (2010)
  • Author(s): 北垣次郎太
  • Organizer: 日本歯周病学会
  • Place of Presentation: 高松市(サンポートホール高松)
  • Year and Date: 2010-09-19
• [Presentation] FGF-2はMAPK依存性にBMPによるSmadリン酸化を制御する (2010)
  • Author(s): 河原貴展、山下元三、橋本悠平、梶川哲宏、前田憲一郎、北垣次郎太、山田聡、北村正博、村上伸也
  • Organizer: 第132回日本歯科保存学会春季学術大会
  • Place of Presentation: 熊本
  • Year and Date: 2010-06-04