Possible Mechanisms of Progression of Periodontal Disease by TACE-Timp3 Interaction in Patients with Diabetes Mellitus

• Project/Area Number: 22792083
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Periodontal dentistry
• Research Institution: Okayama University
• Principal Investigator: OMORI Kazuhiro 岡山大学, 大学院・医歯薬学総合研究科, 助教 (20549860)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
• Keywords: 歯周病 / 糖尿病 / 慢性炎症 / シェディング

Research Abstract

Periodontal disease in poor controlled-diabetic patients has very severe inflammation. In the present study, hyperglycemia enhanced TACE activity and MMP-3 production in human gingival fibroblasts. In addition, recombinant activated MMP-3 enhanced the shedding of soluble IL-6 receptor in macrophage-like differentiated THP-1. These results suggest that hyperglycemia-induced facilitation of TACE and MMP-3 in gingival fibroblasts/macrophage interaction induce severe periodontal inflammation through enhancement of IL-6 signaling in the patients with poor controlled-diabetic mellitus.

Research Products

• [Journal Article] 岡山市医師会・疾患別検査と診断の手引き疾患別シリーズ(15)歯周病「歯周病と生活習慣病」 (2010)
  • Author(s): 高柴正悟,大森一弘
  • Journal Title: 岡山市医師会雑誌 Pages: 115-122
• [Presentation] Activated MMP-3 enhances sIL-6R production in macrophage like differentiated THP-1 cells (2012)
  • Author(s): Hiroya Kobayashi, Kazuhiro Omori, Koji Naruishi, Tomoko Yamaguchi, Hiroshi Maeda, Shogo Takashiba
  • Organizer: 90thInternational Association for Dental Research
  • Place of Presentation: Iguacu falls, Brazil
  • Year and Date: 2012-06-22
• [Presentation] Activated MMP-3 enhances sIL-6R production in macrophage like differentiated THP-1 cells (2012)
  • Author(s): H. Kobayashi, K. Omori et al
  • Organizer: 90th International Association for Dental Research
  • Place of Presentation: Iguacu falls, Brazil
• [Presentation] Activated MMP-3 enhances sIL-6R production in macrophage like differentiated THP-1 cells (2011)
  • Author(s): Hiroya Kobayashi, Kazuhiro Omori, Koji Naruishi, Tomoko Yamaguchi, Hiroshi Maeda, Shogo Takashiba
  • Organizer: 59thJapanese Association for Dental Research
  • Place of Presentation: Hiroshima, Japan
  • Year and Date: 2011-10-08
• [Presentation] Activated MMP-3 enhances sIL-6R production in macrophage like differentiated THP-1 cells (2011)
  • Author(s): H.Kobayashi, K.Omori, et al
  • Organizer: 59th Japanese Association for Dental Research
  • Place of Presentation: 広島国際会議場(広島)
  • Year and Date: 2011-10-08
• [Presentation] 活性型 MMP-3 がマクロファージ様 THP-1 細胞の膜型 IL-6 受容体の発現に及ぼす影響 (2011)
  • Author(s): 小林寛也,大森一弘,成石浩司,山口知子,冨山高史,久保克行,山本大介,前田博史,高柴正悟
  • Organizer: 第133回日本歯科保存学会春季学術大会
  • Place of Presentation: 東京
  • Year and Date: 2011-06-10
• [Presentation] 活性型MMP-3がマクロファージ様THP-1細胞の膜型IL-6受容体の発現に及ぼす影響 (2011)
  • Author(s): 小林寛也, 大森一弘, ら
  • Organizer: 第134回日本歯科保存学会春季学術大会
  • Place of Presentation: 東京ベイ舞浜ホテルクラブリゾート(千葉)
  • Year and Date: 2011-06-10
• [Presentation] MMP-3 によるヒト単球系細胞株THP-1 からの可溶型 IL-6 受容体の産生亢進 (2010)
  • Author(s): 小林寛也,大森一弘,成石浩司,冨山高史,久保克行,山口知子,前田博史,高柴正悟
  • Organizer: 第132回日本歯科保存学会春季学術大会
  • Place of Presentation: 熊本
  • Year and Date: 2010-06-04
• [Presentation] MMP-3によるヒト単球系細胞株THP-1からの可溶型IL-6受容体の産生亢進 (2010)
  • Author(s): 小林寛也, 大森一弘, ら
  • Organizer: 第132回日本歯科保存学会春季学術大会
  • Place of Presentation: 崇城大学市民ホール(熊本)
  • Year and Date: 2010-06-04
• [Presentation] IL-1raによるマウス骨芽細胞様細胞MC3T3-E1におけるIl-1誘導性OPGおよびIL-6産生制御 (2010)
  • Author(s): 久保克行, 大森一弘, ら
  • Organizer: 第53回日本歯周病学会春季学術大会
  • Place of Presentation: 盛岡市民文化ホール(岩手)
  • Year and Date: 2010-05-15