Induced pluripotent stem(iPS) cells in co-culture with periodontal ligament cells
| Project/Area Number |
22792093
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Periodontal dentistry
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| Research Institution | Kagoshima University |
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Principal Investigator |
SAKODA Kenji 鹿児島大学, 医歯学総合研究科, 助教 (70419654)
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| Project Period (FY) |
2010 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2010: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | iPS細胞 / 再生・組織 / 組織幹細胞 / 歯学 / 移植・再生医療 / 細胞・組織 / 発生・分化 |
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| Research Abstract |
The purpose of this study was to induce the periodontal ligament stem cells from iPS cell by co-culture with periodontal ligament cells.
In rat periodontal fenestration model, transplantation of the iPS cells encouraged not only new bone formation but also cementum formation on the denuded dentin surface. In the co-culture system, undifferentiated iPS cells were decreased. We investigated the gene expression using PrimerArray^<(R)>. The differentiated iPS cells increased BMP-2, TGF-β2, and Noggin mRNA expression levels compared with control, while Smad9 and BMPR-1b mRNA expression level were decreased. Further studies are necessary.
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Report
(3 results)
Research Products
(4 results)
