| Project/Area Number |
22792117
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Social dentistry
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| Research Institution | Osaka University |
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Principal Investigator |
SEKINE Shinichi 大阪大学, 大学院・歯学研究科, 助教 (70506344)
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| Project Period (FY) |
2010 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2010: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
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| Keywords | 樹状細胞 / ワクチン / iPS細胞 / 歯周病 / 粘膜ワクチン |
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| Research Abstract |
We have succeeded to generate dendritic cells(DCs) from mouse induced pluripotent stem(iPS) cells according to reported procedures(Senju et al, Stem cells. 27 : 1021, 2009). To assess the whether iPS-DCs possess Ag-presenting Cell(APC) function, OVA-specific naive CD4^+ cells from OT-II Tg mice were purified by FACSAria^<TM>. The iPS cell-derived DCs(iPS-DCs) were suspended in complete RPMI 1640 and incubated with or without OVA for 24hr. Complete RPMI containing OVA was replaced with OVA-specific CD4^+ T cells for 3 days. CD4^+ T cells co-cultured with iPS-DCs showed higher proliferative responses when compared with control. When we examined adjuvanticity of iPS-DCs, significant levels of mucosal IgA Ab responses were also induced. These results demonstrate that iPS-DCs offer an attractive possibility for an effective mucosal vaccine.
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