| Project/Area Number |
22800011
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Neuroscience in general
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| Research Institution | The University of Tokyo |
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Principal Investigator |
NONAKA Mio 東京大学, 大学院・医学系研究科, 特任助教 (30583885)
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| Project Period (FY) |
2010 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
|
| Budget Amount *help |
¥3,146,000 (Direct Cost: ¥2,420,000、Indirect Cost: ¥726,000)
Fiscal Year 2011: ¥1,508,000 (Direct Cost: ¥1,160,000、Indirect Cost: ¥348,000)
Fiscal Year 2010: ¥1,638,000 (Direct Cost: ¥1,260,000、Indirect Cost: ¥378,000)
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| Keywords | 長期記憶 / 遺伝子発現 / シナプス / 転写因子 / 記憶形成 |
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| Research Abstract |
CREB is a critical transcription factor in forming long-lasting memory. CRTC1, a molecule of focus in this study, is the coactivator for CREB. In this project, we have identified phosphorylated sites of CRTC1 that are required for its activity-dependent nuclear translocation in neuronal cells. Next, we investigated the causal relationships between CRTC1 nuclear translocation and the CRE-dependent transcription activity, and revealed that the CREB activation via CRTC1 is independent from the CREB phosphorylation pathway, which had widely been believed to be essential for CRE-dependent gene expression. Lastly, we developed and optimized the in vivo viral injection method and have shown that expressing the constitutively nuclear form of CRTC1 bilaterally in the mouse hippocampus enhanced long-term memory formation.
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