Functional proteomic analysis to identify memory formation-related proteins in the Drosophila mushroom bodies.

Research Project

Project/Area Number	22800012
Research Category	Grant-in-Aid for Research Activity Start-up
Allocation Type	Single-year Grants
Research Field	Neuroscience in general
Research Institution	The University of Tokyo
Principal Investigator	YAMAZAKI Daisuki 東京大学, 分子細胞生物学研究所, 助教 (80588377)
Project Period (FY)	2010 – 2011
Project Status	Completed (Fiscal Year 2011)
Budget Amount *help	¥3,146,000 (Direct Cost: ¥2,420,000、Indirect Cost: ¥726,000) Fiscal Year 2011: ¥1,508,000 (Direct Cost: ¥1,160,000、Indirect Cost: ¥348,000) Fiscal Year 2010: ¥1,638,000 (Direct Cost: ¥1,260,000、Indirect Cost: ¥378,000)
Keywords	記憶学習 / ショウジョウバエ / プロテオミクス / CREB / 記憶・学習
Research Abstract	I started to develop the new methods to approach the molecular or cellular mechanisms underlying memory formation, storage, retrieval in this study. I tried to identify new factors related to learning and memory by proteomics analysis and then, I planed to determine the cells in which they would function. But I failed to establish the methods to concentrate the synapse-specific proteins. So I changed my approach into identify the cells recruited into memory traces. I generated CREB activity reporter flies and succeeded in labeling the cells in response to long-term memory formation.

Report

(3 results)