Analysis of anti-aging mechanism of exercise via histone modifications

• Project/Area Number: 22800062
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Sports science
• Research Institution: Juntendo University
• Principal Investigator: KAWAKAMI Kyojiro 順天堂大学, スポーツ健康科学部, 研究員 (90589227)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥2,756,000 (Direct Cost: ¥2,120,000、Indirect Cost: ¥636,000); Fiscal Year 2011: ¥1,313,000 (Direct Cost: ¥1,010,000、Indirect Cost: ¥303,000); Fiscal Year 2010: ¥1,443,000 (Direct Cost: ¥1,110,000、Indirect Cost: ¥333,000)
• Keywords: ヒストン修飾 / エピジェネティクス / 老化 / 運動 / アセチル化 / リン酸化 / メチル化

Research Abstract

We analyzed anti-aging mechanism of exercise focused on histone modifications. We found dimethylation of histone H3 lysine 4 was decreased in rat gastrocnemius muscle. However, exercise didn’t affect histone modifications. In rat kidney, histone H3 lysine 9 acetylation and serine 10 phosphorylation was increased with age. Moreover, inflammatory genes mRNA were increased with age, and histone H3 lysine 9 acetylation was presented in the vicinity of the gene transcription start site.

Research Products

• [Journal Article] Dietary restriction increases site-specific histone H3 acetylation in rat liver: Possible modulation by sirtuins (2012)
  • Author(s): Kawakami K.、Nakamura A.、Goto S.
  • Journal Title: Biochemical and Biophysical Research Communications Volume: 418 Pages: 836-840
• [Journal Article] Dietary restriction increases site-specific histone H3 acetylation in rat liver : Possible modulation by sirtuins (2012)
  • Author(s): Kyojiro Kawakami, Akihiro Nakamura, Sataro Goto
  • Journal Title: Biochemical and Biophysical Research Communications Volume: 418 Issue: 4 Pages: 836-840
  • DOI: 10.1016/j.bbrc.2012.01.120
  • Peer Reviewed
• [Journal Article] Biological significance of protein Modifications in aging and calorie restriction (2010)
  • Author(s): Nakamura A.、Kawakami K.、Kametani F.、 Nakamoto H、Goto S.
  • Journal Title: Annals of the New York Academy of Sciences Volume: 1197 Pages: 33-39
  • Peer Reviewed
• [Journal Article] Biological significance of protein Modifications in aging and calorie restriction. (2010)
  • Author(s): Nakamura A., Kawakami K., Kametani F., Nakamoto H., Goto S.
  • Journal Title: Annals of the New York Academy of Sciences Volume: 1197 Pages: 33-39
  • Peer Reviewed
• [Presentation] ラット筋肉のヒストン修飾体に対する加齢および運動の影響 (2011)
  • Author(s): 川上恭司郎, 中本英子, 後藤佐多良
  • Organizer: 第34回日本基礎老化学会大会
  • Place of Presentation: 京王プラザホテル(東京都)
  • Year and Date: 2011-06-16
• [Presentation] ラット肝臓のNAD依存性脱アセチル化酵素Sirt1,6に対する加齢と長期食餌制限の影響 (2011)
  • Author(s): 川上恭司郎, 中村明宏, 後藤佐多良
  • Organizer: 第5回日本エピジェネティクス研究会年会
  • Place of Presentation: KKRホテル熊本(熊本県)
  • Year and Date: 2011-05-19
• [Presentation] Effects of aging and dietary restriction on histone H3 modifications in rat liver (2011)
  • Author(s): Kawakami K、Nakamura A、Goto S
  • Organizer: The 10th Japan-Korea Joint Symposium on Cancer and Ageing Research
  • Place of Presentation: 癌研究所(東京都)
• [Presentation] ラット筋肉のヒストン修飾体に対する加齢および運動の影響 (2011)
  • Author(s): 川上恭司郎、中本英子、後藤佐多良
  • Organizer: 第34回日本基礎老化学会大会
  • Place of Presentation: 東京
• [Presentation] ラット肝臓のNAD依存性脱アセチル化酵素Sirt1,6に対する加齢と長期食餌制限の影響 (2011)
  • Author(s): 川上恭司郎、中村明宏、後藤佐多良
  • Organizer: 第5回日本エピジェネティクス研究会年会
  • Place of Presentation: 熊本
• [Presentation] ラット肝臓Sirt1, 6発現量およびNAD/NADH比に対する加齢と長期食餌制限の影響 (2011)
  • Author(s): 川上恭司郎、中村明宏、後藤佐多良
  • Organizer: 日本薬学会第131年会
  • Place of Presentation: 静岡
• [Presentation] Age-related changes of histone H3 modification and effects of lifelong dietary restriction in rat liver (2010)
  • Author(s): Kawakami K、Nakamura A、Nakamoto H、Goto S
  • Organizer: 2010 Spring Conference of the Korean Society for Gerontology and 10th Korea-Japan Gerontologist Joint Meeting
  • Place of Presentation: KRIBB, DaeJeon, (韓国)
  • Year and Date: 2010-07-01
• [Presentation] ラット肝臓のヒストンH3修飾体に対する加齢および長期食餌制限の影響 (2010)
  • Author(s): 川上恭司郎, 中村明宏, 後藤佐多良
  • Organizer: 第33回日本基礎老化学会
  • Place of Presentation: 名古屋大学(愛知県)
  • Year and Date: 2010-06-18
• [Presentation] 加齢および長期食餌制限によるラット肝臓ヒストン修飾の変化 (2010)
  • Author(s): 川上恭司郎, 中村明宏, 後藤佐多良
  • Organizer: 第4回日本エピジェネティクス研究会年会
  • Place of Presentation: 米子市文化ホール(鳥取県)
  • Year and Date: 2010-05-28
• [Presentation] ラット肝臓のヒストンH3修飾体に対する加齢および長期食餌制限の影響 (2010)
  • Author(s): 川上恭司郎、中村明宏、後藤佐多良
  • Organizer: 第33回日本基礎老化学会
  • Place of Presentation: 名古屋
• [Presentation] 加齢および長期食餌制限によるラット肝臓ヒストン修飾の変化 (2010)
  • Author(s): 川上恭司郎、中村明宏、後藤佐多良
  • Organizer: 第4回日本エピジェネティクス研究会年会
  • Place of Presentation: 鳥取
• [Remarks]
  • URL: http://kyojiro.com
• [Remarks]
  • URL: http://kyojiro.com
• [Remarks]
  • URL: http://kyojiro.com