Discovery of biomarker candidates for Cancer stem cell and isolation of the specific antibody

• Project/Area Number: 22800095
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Tumor diagnosis
• Research Institution: 独立行政法人医薬基盤研究所
• Principal Investigator: MURAOKA Satoshi 独立行政法人医薬基盤研究所, プロテオームリサーチプロジェクト, 研究員 (50582681)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥3,146,000 (Direct Cost: ¥2,420,000、Indirect Cost: ¥726,000); Fiscal Year 2011: ¥1,508,000 (Direct Cost: ¥1,160,000、Indirect Cost: ¥348,000); Fiscal Year 2010: ¥1,638,000 (Direct Cost: ¥1,260,000、Indirect Cost: ¥378,000)
• Keywords: 癌幹細胞 / ファージディスプレイ技術 / 次世代質量分析

Research Abstract

Although we had performed to isolate cancer stem cell specific antibody from mouse antibody phage libraries, there were failed. The next idea was to construct human antibody phage library from cancer patients with cancer stem cell. We had finished constructing them. In the future, we will perform to isolate antibody against stomach cancer stem cell.

Research Products

• [Presentation] 乳癌膜タンパク質の大規模プロテオーム解析とSRM/MRMを用いたバイオマーカー候補タンパク質の検証および予後予測診断への応用 (2011)
  • Author(s): 村岡賢、久米秀明、渡邊史生、川崎直子、足立淳、鳴海良平、石飛真人、稲治英生、宮本泰豪、加藤菊也、小寺義男、朝長毅
  • Organizer: 第34回日本分子生物学会
  • Place of Presentation: 横浜
• [Presentation] A strategy for Shotgun Proteomics and SRM-based systematic validation of membrane proteins in breast cancer tissues (2011)
  • Author(s): Satoshi Muraoka, Hideaki Kume, Jun Adachi, Yasuhide Miyamoto, Kikuya Kato Yoshio Kodera, Takeshi Tomonaga
  • Organizer: 第70回日本癌学会
  • Place of Presentation: 名古屋
• [Presentation] 乳癌膜タンパク質の大規模プロテオーム解析とSRM解析による検証 (2011)
  • Author(s): 村岡賢、久米秀明、渡邊史生、川崎直子、足立淳、鳴海良平、石飛真人、稲治英生、宮本泰豪、加藤菊也、小寺義男、朝長毅
  • Organizer: 日本プロテオーム学会2011年大会日本人プロテオーム機構第9回大会
  • Place of Presentation: 新潟
• [Presentation] 乳癌膜タンパク質の大規模プロテオーム解析とSRM解析による検証 (2011)
  • Author(s): 村岡賢
  • Organizer: 日本プロテオーム学会2011年大会
  • Place of Presentation: 新潟
• [Remarks]
  • URL: http://www.nibio.go.jp/proteome/index.html
• [Remarks]
  • URL: http://www.nibio.gojp/proteome/index.html
• [Remarks]
  • URL: http://www.nibio.go.jp/proteome/index.html