Development of Hybrid Cell MEMS by Controlling Coordinated Motion of Vorticella

• Project/Area Number: 22810012
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Microdevices/Nanodevices
• Research Institution: Toyohashi University of Technology
• Principal Investigator: NAGAI Moeto 豊橋技術科学大学, 大学院・工学研究科, 助教 (00580557)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥2,938,000 (Direct Cost: ¥2,260,000、Indirect Cost: ¥678,000); Fiscal Year 2011: ¥1,404,000 (Direct Cost: ¥1,080,000、Indirect Cost: ¥324,000); Fiscal Year 2010: ¥1,534,000 (Direct Cost: ¥1,180,000、Indirect Cost: ¥354,000)
• Keywords: バイオアクチュエータ / マイクロ流体デバイス / ツリガネムシ / バイオMEMS / フローレギュレータ / 協調動作制御 / BioMEMS

Research Abstract

We demonstrate flow regulation powered by a stalk of Vorticella. We introduced Vorticella into a channel and caused the stalks to contract and extend by changing calcium ion concentration. The extended cells transformed the flow through the channel, which shows the controllability of a flow rate.

Research Products

• [Journal Article] Flow Regulator Powered by the Stalk of Vorticella convarallia (2012)
  • Author(s): N Matsumoto, M Nagai, Y Hayasaka, M Oguri, T Kawashima and T Shibata
  • Journal Title: Journal of Physics : Conference Series Volume: vol.352 Pages: 12030-12030
  • Peer Reviewed
• [Journal Article] ツリガネムシの繊毛運動を用いたマイクロミキサの流れ場解析 (2012)
  • Author(s): 早坂陽, 永井萌土, 松本伸賢, 川島貴弘, 柴田隆行
  • Journal Title: 電気学会E部門誌 Volume: vol.132 Pages: 58-63
  • NAID: 10030533395
  • Peer Reviewed
• [Journal Article] Flow Regulator Powered by the Stalk of Vorticella convarallia (2012)
  • Author(s): N Matsumoto, M Nagai, Y Hayasaka, M Oguri, T Kawashima, T Shibata
  • Journal Title: Journal of Physics : Conference Series Volume: 352 Pages: 12030-12030
  • DOI: 10.1088/1742-6596/352/1/012030
  • Peer Reviewed
• [Journal Article] Flow Field Analysis of Micromixer Powered by Ciliary Motion of Vorticella (2012)
  • Author(s): 早坂陽, 永井萌土, 松本伸賢, 川島貴弘, 柴田隆行
  • Journal Title: IEEJ Transactions on Sensors and Micromachines Volume: 132 Issue: 3 Pages: 58-63
  • DOI: 10.1541/ieejsmas.132.58
  • NAID: 10030533395
  • ISSN: 1341-8939, 1347-5525
  • Peer Reviewed
• [Journal Article] Modified imprinting process using hollow microneedle array for forming through holes in polymers (2011)
  • Author(s): Takayuki Shibata, Satoshi Yukizono, Takahiro Kawashima, Moeto Nagai, Toshio Kubota, Mamoru Mita
  • Journal Title: Microelectronic Engineering Volume: 88(In press)
  • Peer Reviewed
• [Journal Article] Experimental and Computational Analysis of Water-Droplet Formation and Ejection Process Using Hollow Microneedle (2011)
  • Author(s): Norihisa Kato, Ryotaro Oka, Takahiro Sakai, Takayuki Shibata, Takahiro Kawashima, Moeto Nagai, Takashi Mineta, Eiji Makino
  • Journal Title: Japanese Journal of Applied Physics Volume: 50(Accepted)
  • NAID: 210000070645
  • Peer Reviewed
• [Journal Article] Contraction and extension of Vorticella and its mechanical characterization under flow loading (2010)
  • Author(s): Moeto Nagai, Hiroshi Asai, and Hiroyuki Fujita
  • Journal Title: Biomicrofluidics Volume: vol.4 Pages: 34109-34109
  • Peer Reviewed
• [Journal Article] Chemical control of Vorticella bioactuator using microfluidics (2010)
  • Author(s): Moeto Nagai, S. angjin Ryu, Todd Thorsen, Paul Matsudaira, and Hiroyuki Fujita
  • Journal Title: Lab on a Chip Volume: vol.10 Pages: 1574-1578
  • Peer Reviewed
• [Journal Article] Chemical control of Vorticella bioactuator using microfluidics (2010)
  • Author(s): Moeto Nagai, Sangjin Ryu, Todd Thorsen, Paul Matsudaira, Hiroyuki Fujita
  • Journal Title: Lab Chip Volume: 10 Pages: 1574-1578
  • Peer Reviewed
• [Journal Article] Contraction and extension of Vorticella and its mechanical characterization under flow loading (2010)
  • Author(s): Moeto Nagai, Hiroshi Asai, Hiroyuki Fujita
  • Journal Title: Biomicrofluidics Volume: 4 Pages: 34109-34109
  • Peer Reviewed
• [Journal Article] マスクレス微細パターン創成技術 (2010)
  • Author(s): 柴田隆行, 川島貴弘, 永井萌土
  • Journal Title: 砥粒加工学会誌 Volume: 54 Pages: 644-647
  • NAID: 10027459016
  • Peer Reviewed
• [Presentation] Cell Manipulation and Intracellular Delivery of Biomolecules for High-throughput Cell Analysis (2012)
  • Author(s): Moeto Nagai
  • Organizer: The 3rd International Symposium on Life Chips
  • Place of Presentation: University of California, Irvine
• [Presentation] Cell Manipulation and Intracellular Delivery of Biomolecules for High-throughput Cell Analysis (2012)
  • Author(s): Moeto Nagai
  • Organizer: The 3rd International Symposium on Life Chips
  • Place of Presentation: University of California, Irvine, California, U.S.A.
• [Presentation] Fabrication of Bioprobe Integrated with Hollow Nanoneedle for Cellular Function Analysis (2011)
  • Author(s): Takayuki Shibata, Norihisa Kato, Shuichi Horiike, Takahiro Kawashima, Moeto Nagai, Takashi Mineta, Eiji Makino
  • Organizer: MEMS 2011 (The 24th International Conference on Micro Electro Mechanical Systems)
  • Place of Presentation: Hilton Cancun Golf and Spa Resort, Cancun, Mexico
  • Year and Date: 2011-01-25
• [Presentation] Cilia of Vorticella for Active Microfluidic Mixing (2011)
  • Author(s): Yo Hayasaka, Moeto Nagai, Nobuyoshi Matsumoto, Takahiro Kawashima, and Takayuki Shibata
  • Organizer: International Workshop on Micro-/Nano-Engineering
  • Place of Presentation: 京都大学桂キャンパス
• [Presentation] Flow Regulator Powered by the Stalk of Vorticella convarallia (2011)
  • Author(s): Nobuyoshi Matsumoto, Moeto Nagai, Yo Hayasaka, Michihito Oguri, Takahiro Kawashima, and Takayuki Shibata
  • Organizer: The Asia-Pacific Interdisciplinary Research Conference
• [Presentation] ツリガネムシの繊毛運動を用いたマイクロミキサの流れ場解析 (2011)
  • Author(s): 早坂陽,永井萌土,松本伸賢,川島貴弘,柴田隆行
  • Organizer: 第28回「センサ・マイクロマシンと応用システム」シンポジウム
• [Presentation] ツリガネムシの運動器官を利用したマイクロ流体素子の開発 (2011)
  • Author(s): 松本伸賢,早坂陽,永井萌土,川島貴弘,柴田隆行
  • Organizer: 平成23年度電気学会センサ・マイクロマシン部門総合研究会
  • Place of Presentation: 東京工業大学すずかげ台キャンパス,神奈川県横浜市
• [Presentation] Flow Regulator Powered by the Stalk of Vorticella convarallia (2011)
  • Author(s): N.Matsumoto, M.Nagai, Y.Hayasaka, M.Oguri, T.Kawashima, T.Shibata
  • Organizer: Asia-Pacific Interdisciplinary Research Conference 2011
  • Place of Presentation: 豊橋技術科学大学
• [Presentation] ツリガネムシの繊毛運動を用いたマイクロミキサの流れ場解析 (2011)
  • Author(s): 早坂陽, 永井萌土, 松本伸賢, 川島貴弘, 柴田隆行
  • Organizer: 第28回「センサとマイクロマシンと応用システム」シンポジウム
  • Place of Presentation: 東京タワーホール船堀
• [Presentation] ツリガネムシの運動器官を利用したマイクロ流体素子の開発 (2011)
  • Author(s): 松本伸賢, 早坂陽, 永井萌土, 川島貴弘, 柴田隆行
  • Organizer: 平成23年度電気学会センサ・マイクロマシン部門総合研究会
  • Place of Presentation: 東京工業大学すずかけ台キャンパス
• [Presentation] オンチップ細胞機能制御のための圧電駆動型マイクロ細胞培養デバイスの開発-細胞導入の効率化と細胞実験の基礎的検討- (2010)
  • Author(s): 山田侑平, 大竹英明, 柴田隆行, 川島貴弘, 永井萌土, 増澤徹, 木村剛, 岸田昌夫
  • Organizer: 第53回自動制御連合講演会
  • Place of Presentation: 高知城ホール,高知県高知市
  • Year and Date: 2010-11-06
• [Presentation] 細胞機能イメージングのためのナノニードル搭載型バイオプローブの開発 (2010)
  • Author(s): 加藤統久, 永井萌土, 川島貴弘, 柴田隆行, 峯田貴, 牧野英司
  • Organizer: 第27回「センサ・マイクロマシンと応用システム」シンポジウム
  • Place of Presentation: くにびきメッセ,島根県松江市
  • Year and Date: 2010-10-14
• [Presentation] An Open Microfluidic Device with Active Valves for Accurate Trapping of DNA by Silicon Nanotweezers (2010)
  • Author(s): Nicolas Lafitte, Momoko Kumemura, Moeto Nagai, Laurent Jalabert, Dominique Collard, Hiroyuki Fujita
  • Organizer: μTAS 2010 (The 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences)
  • Place of Presentation: Martiniplaza, Groningen, Netherlands
  • Year and Date: 2010-10-06
• [Presentation] Feasibility Study of Cell Culture Microdevice Actuated by Piezoelectric Thin Film for On-chip Regulation of Cell Functions (2010)
  • Author(s): Takahiro Kawashima, Takayuki Shibata, Moeto Nagai, Toru Masuzawa, Tsuyoshi Kimura, Akio Kishida
  • Organizer: μTAS 2010 (The 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences)
  • Place of Presentation: Martiniplaza, Groningen, Netherlands
  • Year and Date: 2010-10-04
• [Presentation] 細胞機能解析のためのマイクロニードル搭載型バイオプローブの開発(第6報)-培養液中での細胞の形状イメージング- (2010)
  • Author(s): 堀池修平, 加藤統久, 永井萌土, 川島貴弘, 柴田隆行, 峯田貴, 牧野英司
  • Organizer: 精密工学会2010年度秋季大会
  • Place of Presentation: 名古屋大学,名古屋市千種区
  • Year and Date: 2010-09-28
• [Presentation] マイクロニードルアレイを用いた高品位・高能率マイクロ穴加工技術の開発(第1報)-樹脂フィルムへの貫通穴の形成- (2010)
  • Author(s): 柚木園論, 永井萌土, 川島貴弘, 柴田隆行, 久保田俊夫, 御田護
  • Organizer: 精密工学会2010年度秋季大会
  • Place of Presentation: 名古屋大学, 名古屋市千種区
  • Year and Date: 2010-09-27
• [Presentation] Fabrication of Bioprobe Integrated with Hollow Nanoneedle for Novel AFM Applications in Cellular Function Analysis (2010)
  • Author(s): Norihisa Kato, Shuhei Horiike, Moeto Nagai, Takahiro Kawashima, Takayuki Shibata, Takashi Mineta, Eiji Makino
  • Organizer: Micro and Nano Engineering 2010
  • Place of Presentation: Magazzini del Cotone, Genova, Italy
  • Year and Date: 2010-09-22
• [Presentation] A modified imprinting process using hollow microneedle array for forming through holes in polymers (2010)
  • Author(s): Takayuki Shibata, Satoshi Yukizono, Moeto Nagai, Takahiro Kawashima, Toshio Kubota, Mamoru Mita
  • Organizer: Micro and Nano Engineering 2010
  • Place of Presentation: Magazzini del Cotone, Genova, Italy
  • Year and Date: 2010-09-21
• [Remarks]
• [Remarks]
  • URL: http://www.tut.ac.jp/teach/main.php?mode=detail&article=667
• [Remarks]
  • URL: http://www.tut.ac.jp/teach/main.php?mode=detail&article=667
• [Patent(Industrial Property Rights)] 微細穴加工用工具およびその作製方法ならびに高分子フィルムの加工方法 (2010)
  • Inventor(s): 柴田隆行, 川島貴弘, 永井萌土, 久保田俊夫, 御田護
  • Industrial Property Rights Holder: 豊橋技術科学大学,茨城大学,MEPJ
  • Industrial Property Number: 2010-207803
  • Filing Date: 2010-09-16