| Budget Amount *help |
¥3,016,000 (Direct Cost: ¥2,320,000、Indirect Cost: ¥696,000)
Fiscal Year 2011: ¥1,443,000 (Direct Cost: ¥1,110,000、Indirect Cost: ¥333,000)
Fiscal Year 2010: ¥1,573,000 (Direct Cost: ¥1,210,000、Indirect Cost: ¥363,000)
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| Research Abstract |
Collagen, the most abundant protein in animals, has often been found as animal glue in a wide variety of archaeological materials. In this research project, we propose a methodology for unambiguously identifying animal glues in archeological materials, based on the matrix-assisted laser desorption/ionization time-of-flight(MALDI-TOF) mass spectrometry.
According to ancient records, the animal glue can be prepared from a variety of animals such as cow, deer, fish, donkey, and rabbit. Despite very few substitutions of amino acids found in peptide peaks in the MALDI spectra of tryptic digest of such animal glues, we could distinguish among different animals including cow, deer, fish, and rabbit. Employing these peaks as markers, we identified type I collagen & possibly from cow for the glue of ink sticks manufactured about 270 and 1300 years ago in Nara, Japan.
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