Characterization of foulant in membrane bioreactors for elucidation of fouling mechanisms

• Project/Area Number: 22860001
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Civil and environmental engineering
• Research Institution: Hokkaido University
• Principal Investigator: MIYOSHI Taro 北海道大学, 環境ナノ・バイオ工学研究センター, 博士研究員 (80587791)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥3,146,000 (Direct Cost: ¥2,420,000、Indirect Cost: ¥726,000); Fiscal Year 2011: ¥1,508,000 (Direct Cost: ¥1,160,000、Indirect Cost: ¥348,000); Fiscal Year 2010: ¥1,638,000 (Direct Cost: ¥1,260,000、Indirect Cost: ¥378,000)
• Keywords: 膜分離活性汚泥法 / 膜閉塞 / 膜閉塞成分特性解析 / プロテオーム解析 / MALDI-TOF/MS / レクチンアフィニティークロマトグラフィー

Research Abstract

In this study, detailed characterization of polysaccharides and proteins that caused membrane fouling in membrane bioreactors(MBRs) was carried out. A mild acid hydrolysis of polysaccharides eluted from a lectin column followed by MALDI-TOF/MS analysis allowed us to have deeper insights into structures of polysaccharides that have high fouling potentials. The proteins extracted from fouled membranes were successfully concentrated by the combination of a crude concentration with ultra-filtration(UF) membrane and trichloroacetic acid(TCA) precipitation. The concentrated proteins were separated well by 2D-PAGE. Analyzing selected 2D-PAGE spots by N-terminal amino acid sequencing analysis led to the identification of two well-characterized outer membrane proteins originating from Pseudomonas genus

Research Products

• [Journal Article] Characteristics of proteins involved in membrane fouling in membrane bioreactors(MBRs) treating municipal wastewater : the application of metaproteomic analyses (2011)
  • Author(s): Taro Miyoshi, Tomoyasu Aizawa, Katsuki Kimura, Yoshimasa Watanabe
  • Journal Title: Desalination and Water Treatment Volume: 34 Pages: 150-155
  • NAID: 120005228167
  • Peer Reviewed
• [Journal Article] Characteristics of proteins involved in membrane fouling in membrane bioreactors (MBRs) treating municipal wastewater : the application of metaproteomic analyses (2011)
  • Author(s): Taro Miyoshi, Tomoyasu Aizawa, Katsuki Kimura, Yoshimasa Watanabe
  • Journal Title: Desalination and Water Treatment Volume: 34 Pages: 150-155
  • NAID: 120005228167
  • Peer Reviewed
• [Presentation] MBR膜ファウリング多糖のMALDI-TOFMS分析における試料調製条件の検討 (2012)
  • Author(s): 田村尚也
  • Organizer: 水環境学会年会
  • Place of Presentation: 東洋大学、東京
  • Year and Date: 2012-03-16
• [Presentation] 膜分離活性汚泥法において膜ファウリングを引き起こしているタンパク質のメタプロテオーム解析 (2011)
  • Author(s): 三好太郎、相沢智康、木村克輝、渡辺義公
  • Organizer: 土木学会全国大会
  • Place of Presentation: 愛媛大学、松山
  • Year and Date: 2011-09-09
• [Presentation] 膜分離活性汚泥法において膜ファウリングに関与しているタンパク質の構造解析を通じた膜ファウリング発生機構の検討 (2011)
  • Author(s): 三好太郎、相沢智康、木村克輝、渡辺義公
  • Organizer: 水環境学会年会
  • Place of Presentation: 北海道大学、札幌
  • Year and Date: 2011-03-18
• [Presentation] MBRの膜ファウリングを引き起こす糖類の特性解析 (2011)
  • Author(s): 田中一平、木村克輝、三好太郎、渡辺義公
  • Organizer: 水環境学会年会
  • Place of Presentation: 北海道大学、札幌
  • Year and Date: 2011-03-18
• [Presentation] 膜分離活性汚泥法において膜ファウリングに関与しているタンパク質の構造解析を通じた膜ファウリング発生機構の検討 (2011)
  • Author(s): 三好太郎
  • Organizer: 第45回日本水環境学会年会
  • Place of Presentation: 北海道大学、札幌
  • Year and Date: 2011-03-18
• [Presentation] MBRの膜ファウリングを引き起こす糖類の特性解析 (2011)
  • Author(s): 田中一平
  • Organizer: 第45回日本水環境学会年会
  • Place of Presentation: 北海道大学、札幌
  • Year and Date: 2011-03-18
• [Presentation] MBR膜ファウリング多糖のMALDI-TOFMS分析における試料調製条件の検討 (2011)
  • Author(s): 田村尚哉、木村克輝、三好太郎、渡辺義公
  • Organizer: 水環境学会年会
  • Place of Presentation: 東洋大学、東京
  • Year and Date: 2011-03-16
• [Presentation] Characteristics of proteins involved in membrane fouling in membrane bioreactors(MBRs) treating municipal wastewater : the application of metaproteomic analyses (2010)
  • Author(s): Taro Miyoshi, Tomoyasu Aizawa, Katsuki Kimura, Yoshimasa Watanabe
  • Organizer: The 6th Conference of the Aseanian Membrane Society In Conjunction with the 7th International Membrane Science and Technology Conference
  • Place of Presentation: Hilton Sydney, Sydney
  • Year and Date: 2010-11-24
• [Presentation] Characteristics of proteins involved in membrane fouling in membrane bioreactors (MBRs) treating municipal wastewater : the application of metaproteomic analyses (2010)
  • Author(s): Taro Miyoshi
  • Organizer: The 6^<th> Conference of the Aseanian Membrane Society In Conjunction with the 7^<th> International Membrane Science and Technology Conference
  • Place of Presentation: Hilton Sydney, Sydney Australia
  • Year and Date: 2010-11-24
• [Presentation] 膜分離活性汚泥法において膜ファウリングに関与しているタンパク質の構造解析 (2010)
  • Author(s): 三好太郎、相沢智康、木村克輝、渡辺義公
  • Organizer: 環境工学研究フォーラム
  • Place of Presentation: 高知大学、高知
  • Year and Date: 2010-11-13
• [Presentation] 膜分離活性汚泥法において膜ファウリングに関与しているタンパク質の構造解析 (2010)
  • Author(s): 三好太郎
  • Organizer: 第47回環境工学研究フォーラム
  • Place of Presentation: 高知大学、高知
  • Year and Date: 2010-11-13
• [Presentation] Metaproteomic analysis of proteins causing physically irreversible fouling in membrane bioreactors (MBRs) (2010)
  • Author(s): Taro Miyoshi
  • Organizer: Membranes in Drinking and Industrial Water Treatment
  • Place of Presentation: Rica Nidelven Hotel Trondheim, Norway
  • Year and Date: 2010-06-28