Monitoring of cellular response to applied mechanical stimulus in bone cells by infrared spectroscopy

• Project/Area Number: 22860002
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Materials/Mechanics of materials
• Research Institution: Tohoku University
• Principal Investigator: AONUMA Yuki 東北大学, 電気通信研究所, 助教 (80582262)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥2,535,000 (Direct Cost: ¥1,950,000、Indirect Cost: ¥585,000); Fiscal Year 2011: ¥1,105,000 (Direct Cost: ¥850,000、Indirect Cost: ¥255,000); Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: 細胞情報・動態 / 骨芽細胞 / 赤外分光 / 細胞分化 / ATP合成

Research Abstract

This study has aimed to apply infrared spectroscopy as a label free and real-time monitoring method for detecting cellular response to applied mechanical stimulus in bone cells. Infrared light penetrated through semiconductor prism was probed activity of adhesive cells on the prism as infrared absorption. In addition, live cell monitoring apparatus was fabricated for detecting kinetic information of target cells, which was applicable to experimental system to apply fluid flow stimulation.

Research Products

• [Journal Article] Real-time monitoring of mitochondrial adenosine 5'-triphosphate synthesis and hydrolysis by surface infrared spectroscopy (2011)
  • Author(s): Ryo-taro Yamaguchi, Ayumi Hirano-Iwata, Yuki Aonuma, Yuya Yoshimura, Yasuo Shinohara, Yasuo Kimura and Michio Niwano
  • Journal Title: Applied Physics Letter Volume: Vol.98, No.13 Pages: 133703-133703
  • Peer Reviewed
• [Journal Article] Real-time monitoring of mitochondrial adenosine 5'-triphosphate synthesis and hydrolysis by surface infrared spectroscopy (2011)
  • Author(s): Ryo-taro Yamaguchi, et al
  • Journal Title: Applied Physics Letter Volume: Vol.98
  • Peer Reviewed
• [Presentation] 表面赤外分光法を用いた脂肪細胞分化過程の非標識計測 (2012)
  • Author(s): 青沼有紀, 近藤康彦, 平野愛弓, 木村康男, 篠原康雄, 庭野道夫
  • Organizer: 平成23年度日本表面科学会東北・北海道支部学術講演会
  • Place of Presentation: 仙台
  • Year and Date: 2012-03-09
• [Presentation] 表面赤外分光法を用いた脂肪細胞分化過程の非標識計測 (2012)
  • Author(s): 青沼有紀
  • Organizer: 日本表面科学会東北・北海道支部学術講演会
  • Place of Presentation: 仙台
  • Year and Date: 2012-03-09
• [Presentation] Long-term Monitoring of Cell Differentiation Using Surface Infrared Spectroscopy (2011)
  • Author(s): Yuki Aonuma, Yasuhiko Kondo, Ayumi Hirano-Iwata, Yasuo Kimura, Yasuo Shinohara and Michio Niwano
  • Organizer: The Sixth International Symposium on Surface Science
  • Place of Presentation: Tokyo
  • Year and Date: 2011-12-14
• [Presentation] Long-term Monitoring of Cell Differentiation Using Surface Infrared Spectroscopy (2011)
  • Author(s): Yuki Aonuma
  • Organizer: The 6^<th> International Symposiumon Surface Science
  • Place of Presentation: Tokyo
  • Year and Date: 2011-12-14
• [Presentation] 表面赤外分光法に基づく細胞分化過程の非標識観測 (2011)
  • Author(s): 青沼有紀, 近藤康彦, 平野愛弓, 木村康男, 篠原康雄, 庭野道夫
  • Organizer: 第72回応用物理学会学術講演会
  • Place of Presentation: 山形
  • Year and Date: 2011-08-31
• [Presentation] 表面赤外分光法を用いたミトコンドリア内ATP合成過程のリアルタイム評価 (2010)
  • Author(s): 青沼有紀, 山口僚太郎, 阿部真帆, 平野愛弓, 木村康男, 篠原康雄, 庭野道夫
  • Organizer: 第30回表面科学学術講演会
  • Place of Presentation: 大阪
  • Year and Date: 2010-11-04
• [Presentation] 表面赤外分光法を用いたミトコンドリア内ATP合成過程のリアルタイム評価 (2010)
  • Author(s): 青沼有紀, ほか
  • Organizer: 第30回表面科学学術講演会
  • Place of Presentation: 大阪大学コンベンションセンター(大阪府)
  • Year and Date: 2010-11-04
• [Presentation] Surface Infrared Spectroscopic Study of ATP Synthesis in Mitochondria (2010)
  • Author(s): Yuki Aonuma, Ryo-taro Yamaguchi, Maho Abe, Ayumi Hirano-Iwata, Yasuo Kimura, Yasuo Shinohara and Niwano Michio
  • Organizer: 2010 International Conference on Solid State Devices and Materials
  • Place of Presentation: Tokyo
  • Year and Date: 2010-09-23
• [Presentation] Surface infrared spectroscopic study of ATP synthesis in mitochondria (2010)
  • Author(s): Yuki Aonuma, et al
  • Organizer: 2010 International Conference on Solid State Devices and Materials
  • Place of Presentation: The University of Tokyo (Tokyo)
  • Year and Date: 2010-09-23