| Budget Amount *help |
¥3,146,000 (Direct Cost: ¥2,420,000、Indirect Cost: ¥726,000)
Fiscal Year 2011: ¥1,508,000 (Direct Cost: ¥1,160,000、Indirect Cost: ¥348,000)
Fiscal Year 2010: ¥1,638,000 (Direct Cost: ¥1,260,000、Indirect Cost: ¥378,000)
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| Research Abstract |
We have been focusing on the structural and functional analysis of GatCAB, which has an important role in an indirect pathway of aminoacyl-tRNA synthesis. We have determined the crystal structure of an archaeal GatCAB at 2. 5 A resolution. The structural comparison of the archaeal GatCAB and the bacterial GatCAB suggested that a 3_<10> helix in the bacterial GatCAB is crucial for recognition the U1-A72 pair in tRNA^<Gln>, and the absence of the helix in the archaeal GatCAB enables the enzyme recognize aa-tRNA with G1-C72 base-pairs.
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