Analysis of thioredoxin mediated redox-regulation of nitrogenase activity

• Project/Area Number: 22870009
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: Tokyo Institute of Technology
• Principal Investigator: NOMATA Jiro 東京工業大学, 資源化学研究所, 助教 (40583216)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥3,146,000 (Direct Cost: ¥2,420,000、Indirect Cost: ¥726,000); Fiscal Year 2011: ¥1,508,000 (Direct Cost: ¥1,160,000、Indirect Cost: ¥348,000); Fiscal Year 2010: ¥1,638,000 (Direct Cost: ¥1,260,000、Indirect Cost: ¥378,000)
• Keywords: レドックス / 酸素感受性 / シアノバクテリア / 生理学 / 酵素

Research Abstract

Fe-protein, a reductant component of nitrogenase, was expressed in E. coli and successfully purified under anaerobic condition. 2. Thioredoxin A was expressed in E. coli and successfully purified in active form.

Research Products

• [Presentation] Effect of dioxygen on the redox-study (2012)
  • Author(s): Nomata J
  • Organizer: International Symposium on Innovative Nanobiodevices 2012
  • Place of Presentation: Nagoya univ.
  • Year and Date: 2012-03-22
• [Presentation] ニトロゲナーゼ類似型プロトクロロフィリド還元酵素の反応機構の解析 (2012)
  • Author(s): 野亦次郎
  • Organizer: 日本植物生理学会
  • Place of Presentation: 京都産業大学
  • Year and Date: 2012-03-18
• [Presentation] ニトロゲナーゼ類似型プロトクロロフィリド還元酵素の反応機構の解析 (2012)
  • Author(s): 野亦次郎、近藤徹、溝口正、民秋均、伊藤繁、藤田祐一
  • Organizer: 日本植物生理学会(第53回)
  • Place of Presentation: 京都産業大学(京都)
• [Presentation] Effect of dioxygen on the redox-study (2012)
  • Author(s): Jiro Nomata and Toru Hisabori
  • Organizer: International Symposium on Innovative Nanobiodevices
  • Place of Presentation: Nagoya univ.(Nagoya)
• [Presentation] ニトロゲナーゼ類似型プロトクロロフィリド還元酵素の反応機構の解析 (2011)
  • Author(s): 野亦次郎・近藤徹・溝口正・民秋均・伊藤繁・藤田祐一
  • Organizer: 日本植物生理学会
  • Place of Presentation: 東北大学 (仙台)
  • Year and Date: 2011-03-20