| Budget Amount *help |
¥3,146,000 (Direct Cost: ¥2,420,000、Indirect Cost: ¥726,000)
Fiscal Year 2011: ¥1,508,000 (Direct Cost: ¥1,160,000、Indirect Cost: ¥348,000)
Fiscal Year 2010: ¥1,638,000 (Direct Cost: ¥1,260,000、Indirect Cost: ¥378,000)
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| Research Abstract |
The central spindle forms between segregating chromosomes during anaphase and is required for proper coupling of karyokinesis and cytokinesis. It remains largely unknown how this microtubule-based structure is prepared during anaphase. Using live imaging of MT plus ends and a MT depolymerization and regrowth assay, we show that de novo MT generation in the interchromosomal region during anaphase is important for central spindle formation in human cells. Generation of interchromosomal MTs and subsequent formation of the central spindle require augmin, a protein complex implicated in nucleation of noncentrosomal MTs during pre-anaphase. MTs generated in an HURP-dependent manner during anaphase also contribute to central spindle MT formation redundantly with pre-anaphase MTs. Based upon these results, I could established a new model for central spindle assembly.
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