• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

放射光真空紫外円二色性による蛋白質の精密構造解析

Research Project

Project/Area Number 22870021
Research Category

Grant-in-Aid for Research Activity Start-up

Allocation TypeSingle-year Grants
Research Field Structural biochemistry
Research InstitutionHiroshima University

Principal Investigator

松尾 光一  広島大学, 放射光科学研究センター, 特任助教 (40403620)

Project Period (FY) 2010 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2011: ¥1,495,000 (Direct Cost: ¥1,150,000、Indirect Cost: ¥345,000)
Fiscal Year 2010: ¥1,625,000 (Direct Cost: ¥1,250,000、Indirect Cost: ¥375,000)
Keywords放射光 / 真空紫外 / 円二色性 / 蛋白質 / アミロイド線維 / バイオインフォマティクス / 分子動力学 / 生体膜 / ニューラルネットワーク法
Research Abstract

今年度は,VUVCD分光法を分子動力学法(MD)やBioinformatics技術等の計算科学と組み合わせることで,アミロイド線維の構造解析や生体膜と結合した蛋白質の構造解析に応用した。
(1)β_2-microglobulinペプチドのアミロイド線維の構造解析:アミロイド化したβ_2-microglobulin(β_2-m)の断片ペプチド(β_2-m_<21-29>)の構造を,VUVCDとGromacsを使用し解析した。β_2-m_<21-29>は,β-sheet構造特有のVUVCDスペクトルを示したが,二次構造解析からは,実測スペクトルは再現できず,二次構造以外のCD成分の存在が示唆された。これは,β_2-m_<21-29>スペクトルに側鎖由来のCDが大きく寄与するという昨年度のMDとCD理論から得られた結果を支持していた。また,ジスルフィド結合の有無及びsheet間の平行や逆平行・芳香族残基側鎖の環境を考慮し,β_2-m_<21-29>アミロイド線維構造をシミュレート(20ns)した結果,実測スペクトルに近い理論CDを得ることに成功した。
(2)膜により誘起された蛋白質の溶液構造解析:天然変性蛋白質であるミエリン塩基性蛋白質(MBP)の生体膜存在下・非存在下でのVUVCDスペクトル測定を行い,MBPが生体膜との相互作用によりヘリックスリッチなスペクトルを示すことを確認した。また,VUVCD-NN法によるアミノ酸配列レベルでの二次構造解析から,生体膜相互作用により起こる構造変化を明確にした。現在,分子動力学を用いた構造解析の準備段階にある。
これらの結果は、VUVCD・分子動力学・CD理論の組み合せ法が,これまで解析が困難であったアミロイド線維等の構造解析に有効であることを示し,またこの手法が,生体膜と結合した蛋白質の構造解析にも応用できることを示している。

Report

(2 results)
  • 2011 Annual Research Report
  • 2010 Annual Research Report
  • Research Products

    (29 results)

All 2012 2011 2010 Other

All Journal Article (15 results) (of which Peer Reviewed: 4 results) Presentation (13 results) Remarks (1 results)

  • [Journal Article] Secondary-structure analysis of alcohol-denatured proteins by vacuum-ultraviolet circular dichroism spectroscopy2012

    • Author(s)
      K.Matsuo
    • Journal Title

      Proteins

      Volume: 80 Pages: 281-293

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Experimental and Theoretical Studies of Vacuum-Ultraviolet Electronic Circular Dichroism of Hydroxy Acids in Aqueous Solution2011

    • Author(s)
      T.Fukuyama
    • Journal Title

      Chirality

      Volume: 23

    • Related Report
      2011 Annual Research Report 2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Spectroscopic characterization of intermolecular interaction of amyloid β promoted on GM1 micelles.2011

    • Author(s)
      Yagi-Utsumi M. et al. (Yanagisawa K., Kato K.)
    • Journal Title

      Int J Alzheimers Dis.

      Volume: 2011 Issue: 1

    • DOI

      10.4061/2011/925073

    • Related Report
      2011 Annual Research Report 2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Structural analysis of methyl α-D-glucopyranoside in aqueous solution by vacuum-ultraviolet circular dichroism and time-dependent density functional theory2011

    • Author(s)
      Koichi Matsuo
    • Journal Title

      HiSOR Activity Report

      Volume: 2010 Pages: 142-143

    • Related Report
      2011 Annual Research Report
  • [Journal Article] Vacuum-ultraviolet circular dichroism spectra of adenosine ribonucleotides in aqueous solution2011

    • Author(s)
      Koichi Matsuo
    • Journal Title

      HiSOR Activity Report

      Volume: 2010 Pages: 144-145

    • Related Report
      2011 Annual Research Report
  • [Journal Article] VUV-CD spectral analysis of PBAN (24-33)-NH_2 and -OH2011

    • Author(s)
      K.Nagata
    • Journal Title

      HiSOR Activity Report

      Volume: 2010 Pages: 146-147

    • Related Report
      2011 Annual Research Report
  • [Journal Article] Spectroscopic characterization of inter-molecular interaction of amyloid β promoted on GM1 micelles2011

    • Author(s)
      M.Yagi-Utsumi
    • Journal Title

      HiSOR Activity Report

      Volume: 2010 Pages: 148-149

    • Related Report
      2011 Annual Research Report
  • [Journal Article] Circular dichroism spectra of amino-acid films irradiated with spin polarized electrons in beta-decay radiation2011

    • Author(s)
      J.Takahashi
    • Journal Title

      HiSOR Activity Report

      Volume: 2010 Pages: 150-151

    • Related Report
      2011 Annual Research Report
  • [Journal Article] Secondary structure analyses of human erythrocyte band 3 by VU VCD spectroscopy2011

    • Author(s)
      Y.Ikeda
    • Journal Title

      HiSOR Activity Report

      Volume: 2010 Pages: 154-155

    • Related Report
      2011 Annual Research Report
  • [Journal Article] Secondary-structure analysis of bacteriorhodopsin by vacuum-ultraviolet circular dichroism spectroscopy2011

    • Author(s)
      H.Muranaka
    • Journal Title

      HiSOR Activity Report

      Volume: 2010 Pages: 156-157

    • Related Report
      2011 Annual Research Report
  • [Journal Article] Conformational change of bovine carbonic anhydrase II by vacuum-ultraviolet circular dichroism spectroscopy2011

    • Author(s)
      H.Muranaka
    • Journal Title

      HiSOR Activity Report

      Volume: 2010 Pages: 158-158

    • Related Report
      2011 Annual Research Report
  • [Journal Article] Contribution of aromatic side chains to the vacuum-ultraviolet circular dichroism spectra of proteins2011

    • Author(s)
      E.Ohmae
    • Journal Title

      HiSOR Activity Report

      Volume: 2010 Pages: 159-159

    • Related Report
      2011 Annual Research Report
  • [Journal Article] 放射光円二色性分光による生体分子構造解析のブレークスルー2010

    • Author(s)
      松尾光一
    • Journal Title

      化学と工業

      Volume: 63 Pages: 652-652

    • NAID

      10026510002

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 放射光円二色性分光法による蛋白質の精密構造解析と医薬分野への応用2010

    • Author(s)
      松尾光一
    • Journal Title

      BIO INDUSTRY

      Volume: 27 Pages: 63-70

    • Related Report
      2010 Annual Research Report
  • [Journal Article] Analysis of Protein Solution Structures using Synchrotron-Radiation Circular Dichroism and Application to Medical Fields2010

    • Author(s)
      Koichi Matsuo
    • Journal Title

      HiSOR Activity Report

      Volume: 2009 Pages: 98-99

    • Related Report
      2010 Annual Research Report
  • [Presentation] 高い異方性を持つコラーゲンシートのキラル光学的性質2011

    • Author(s)
      中川鉄馬
    • Organizer
      第1回CSJ化学フェスタ
    • Place of Presentation
      東京
    • Year and Date
      2011-11-15
    • Related Report
      2011 Annual Research Report
  • [Presentation] Vacuum-ultraviolet circular dichroism of methyl a-D-glucopyranoside in aqueous solution investigated by time-dependent density functional theory2011

    • Author(s)
      Koichi Matsuo
    • Organizer
      The 71st Okazaki Conference
    • Place of Presentation
      Okazaki
    • Year and Date
      2011-10-13
    • Related Report
      2011 Annual Research Report
  • [Presentation] Structural analysis of carbohydrates by synchrotron-radiation vacuum-ultraviolet circular dichroism spectroscopy2011

    • Author(s)
      Kunihiko Gekko
    • Organizer
      The 71st Okazaki Conference
    • Place of Presentation
      Okazaki(招待講演)
    • Year and Date
      2011-10-12
    • Related Report
      2011 Annual Research Report
  • [Presentation] Solution-Structure Analysis of Methyl α-_D-Glucopyranoside by Vacuum-Ultraviolet Circular Dichroism Spectroscopy and Time-Dependent Density Functional Theory2011

    • Author(s)
      Koichi Matsuo
    • Organizer
      13th International Conference on Circular Dichroism
    • Place of Presentation
      OXFORD(英国)
    • Year and Date
      2011-07-26
    • Related Report
      2011 Annual Research Report
  • [Presentation] Optical properties in highly anisotropic collagen sheets2011

    • Author(s)
      K.Nakagawa
    • Organizer
      Symposium on Molecular Chirality
    • Place of Presentation
      Tokyo
    • Year and Date
      2011-05-21
    • Related Report
      2011 Annual Research Report
  • [Presentation] β_2-Microglobulinフラグメントが形成するアミロイド線維構造の真空紫外CD解析2011

    • Author(s)
      松尾光一
    • Organizer
      第3回日本生物物理学会中国四国支部大会
    • Place of Presentation
      東広島
    • Year and Date
      2011-05-14
    • Related Report
      2011 Annual Research Report
  • [Presentation] Circular Dichroism Spectra of Amino-acid Films irradiated with Spin Polarized Electrons in Beta-decay Radiation2011

    • Author(s)
      Jun-ichi Takahashi
    • Organizer
      The 15th Hiroshima International Symposium on Synchrotron Radiation
    • Place of Presentation
      東広島
    • Year and Date
      2011-03-03
    • Related Report
      2010 Annual Research Report
  • [Presentation] Structural Analysis of Methyl α-D-Glucopyranoside in Aqueous Solution by Vacuum-ultraviolet Circular Dichroism Spectroscopy and Time-dependent Density Functional Theory2011

    • Author(s)
      Koichi Matsuo
    • Organizer
      The 15th Hiroshima International Symposium on Synchrotron Radiation
    • Place of Presentation
      東広島
    • Year and Date
      2011-03-03
    • Related Report
      2010 Annual Research Report
  • [Presentation] β_2-Microglobulinアミロイド線維フラグメントの真空紫外円二色性スペクトルの理論解析2011

    • Author(s)
      松尾光一
    • Organizer
      第24回日本放射光学会年会・放射光科学合同シンポジウム
    • Place of Presentation
      筑波
    • Year and Date
      2011-01-09
    • Related Report
      2010 Annual Research Report
  • [Presentation] 蛋白質の真空紫外円二色性に及ぼすTrp側鎖のExciton効果2011

    • Author(s)
      大前英司
    • Organizer
      日本農芸化学会大会2011
    • Place of Presentation
      京都
    • Related Report
      2010 Annual Research Report
  • [Presentation] Structural Analysis of Core Fragment of β_2-Microglobulin Amyloid Fibrils by Vacuum-Ultraviolet CD Spectroscopy2010

    • Author(s)
      Koichi Matsuo
    • Organizer
      The 48th Annual Meeting of the Biophysical Society of Japan
    • Place of Presentation
      仙台
    • Year and Date
      2010-09-20
    • Related Report
      2010 Annual Research Report
  • [Presentation] Solution-Structure Analysis of Saccharides by Synchrotron-Radiation Vacuum-Ultraviolet Circular Dichroism Spectroscopy2010

    • Author(s)
      Koichi Matsuo
    • Organizer
      The 37th International conference on Vacuum Ultraviolet and X-ray Physics
    • Place of Presentation
      Vancouver, Canada
    • Year and Date
      2010-07-13
    • Related Report
      2010 Annual Research Report
  • [Presentation] Experimental and Theoretical Studies on Vacuum-ultraviolet Circular Dichroism of Hydroxyl Acids in Aqueous Solution2010

    • Author(s)
      Takayuki Fukuyama
    • Organizer
      The 22nd International Symposium on Chriality
    • Place of Presentation
      札幌
    • Related Report
      2010 Annual Research Report
  • [Remarks]

    • URL

      http://www.hsrc.hiroshima-u.ac.jp/

    • Related Report
      2010 Annual Research Report

URL: 

Published: 2010-08-27   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi