Simultaneous observation of conformational changes and the chemical reaction of F1-ATPase under the TIRF microscopy with high-speed polarization modulation
| Project/Area Number |
22870028
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Biophysics
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| Research Institution | Gakushuin University |
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Principal Investigator |
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| Research Collaborator |
NISHIZAKA Takayuki 学習院大学, 理学部物理学科, 教授 (40359112)
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| Project Period (FY) |
2010 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥3,146,000 (Direct Cost: ¥2,420,000、Indirect Cost: ¥726,000)
Fiscal Year 2011: ¥1,508,000 (Direct Cost: ¥1,160,000、Indirect Cost: ¥348,000)
Fiscal Year 2010: ¥1,638,000 (Direct Cost: ¥1,260,000、Indirect Cost: ¥378,000)
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| Keywords | 構造変化 / 全反射型顕微鏡 / 1分子観察 / F1-ATPase / モーター蛋白質 / 回転 / Cy3-ATP / モーター蛋白 / 偏光 / ATP加水分解 |
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| Research Abstract |
The purpose of the present study is to correlate conformational changes of an enzyme with chemical reaction steps. We linked a fluorescent molecule to F1-ATPase as a probe of conformations and detected its changes in orientation under the optical microscope. By improvements of the microscope, we achieved an angle resolution of 2 degrees and a temporal resolution of 0.1 s. By combining this microscope with magnetic tweezers, differences in conformational profiles during different chemical pathways were indicated.
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Report
(3 results)
Research Products
(39 results)
