Photo-control of myosin V using photo-responsive calmodulin

• Project/Area Number: 22870030
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Biophysics
• Research Institution: Soka University
• Principal Investigator: SHISHIDO Hideki 創価大学, 工学部, 助教 (30580120)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥2,938,000 (Direct Cost: ¥2,260,000、Indirect Cost: ¥678,000); Fiscal Year 2011: ¥1,482,000 (Direct Cost: ¥1,140,000、Indirect Cost: ¥342,000); Fiscal Year 2010: ¥1,456,000 (Direct Cost: ¥1,120,000、Indirect Cost: ¥336,000)
• Keywords: 分子モーター / バイオテクノロジー / 光スイッチ / 生体分子

Research Abstract

In this study, we tried to photo-control myosin V as calmodulin target protein using photoresponsive calmodulin, which is modified with photochromic molecule. As a result, we successfully photo-controlled ATPase activity of myosin V.

Research Products

• [Journal Article] PHOTOCONTROL OF MOTOR PROTEINS USING PHOTO-RESPONSIBLE CALMODULIN DIMER (2012)
  • Author(s): Hideki Shishido, Hideo Seo and Shinsaku Maruta
  • Journal Title: Biophysical Journal Volume: Vol.102 Issue: 3 Pages: 371a-371a
  • DOI: 10.1016/j.bpj.2011.11.2025
• [Journal Article] Engineering of a novel Ca^<2+>-regulated kinesin molecular motor using a calmodulin dimer linker (2012)
  • Author(s): Hideki Shishido
  • Journal Title: Biochemical and Biophysical Research Communications Volume: VOL.423 Issue: 2 Pages: 386-391
  • DOI: 10.1016/j.bbrc.2012.05.135
  • Peer Reviewed
• [Journal Article] PHOTOCONTROL OF MYOSIN V ATPASE ACTIVITY USING CALMODULIN MODIFIED WITH PHOTOCHROMIC COMPOUND (2011)
  • Author(s): Hideki Shishido, Mitsuo Ikebe and Shinsaku Maruta
  • Journal Title: Biophysical Journal Volume: Vol.100 Issue: 3 Pages: 487a-487a
  • DOI: 10.1016/j.bpj.2010.12.2854
• [Journal Article] Photocontrol of Myosin V ATPase Activity using Calmodulin Modified with Photochromic Compound (2011)
  • Author(s): Hideki Shishido
  • Journal Title: Biophysical Journal Volume: VOL.100
• [Journal Article] Engineering of a novel Ca2+-regulated kinesin molecular motor using a calmodulin dimer linker
  • Author(s): Hideki Shishido and Shinsaku Maruta
  • Journal Title: Biochemical and Biophysical Research Communications Volume: (印刷中)
  • Peer Reviewed
• [Presentation] Photocontrol of motor proteins using photo-responsive calmodulin dimer (2012)
  • Author(s): Hideki Shishido, Hideo Seo and Shinsaku Maruta
  • Organizer: Biophysical Society
  • Place of Presentation: San Diego Convention Center(CA, USA)
  • Year and Date: 2012-02-27
• [Presentation] PHOTOCONTROL OF MOTOR PROTEINS USING PHOTO-RESPONSIBLE CALMODULIN DIMER (2012)
  • Author(s): Hideki Shishido
  • Organizer: Biophysical Society
  • Place of Presentation: San Diego Convention Center (CA, USA)
  • Year and Date: 2012-02-27
• [Presentation] Photocontrol of Myosin V activity using Photo-responsible Calmodulin (2011)
  • Author(s): Hideki Shishido, Shinsaku Maruta
  • Organizer: 日本生物物理学会
  • Place of Presentation: 兵庫県立大学(兵庫県)
  • Year and Date: 2011-09-18
• [Presentation] Photocontrol of Myosin V ATPase activity using Calmodulin modified with Photochromic compound (2011)
  • Author(s): Hideki Shishido, Mitsuo Ikebe and Shinsaku Maruta
  • Organizer: Biophysical Society
  • Place of Presentation: Baltimore Convention Center(MD, USA)
  • Year and Date: 2011-03-08
• [Presentation] Photocontrol of Myosin V ATPase Activity using Calmodulin Modified with Photochromic Compound (2011)
  • Author(s): Hideki Shishido
  • Organizer: Biophysical Society
  • Place of Presentation: Baltimore Conventione Center (MD, USA)
  • Year and Date: 2011-03-08
• [Presentation] Photocontrol of Myosin V ATPase using Calmodulin modified with Azobenzene derivative (2010)
  • Author(s): Hideki Shishido, Shinsaku Maruta
  • Organizer: 日本生物物理学会
  • Place of Presentation: 東北大学(宮城県)
  • Year and Date: 2010-09-20
• [Presentation] アゾベンゼン導入カルモジュリンを用いたミオシンVのATPase活性の光制御 (2010)
  • Author(s): 宍戸英樹
  • Organizer: 日本生物物理学会
  • Place of Presentation: 東北大学(宮城県)
  • Year and Date: 2010-09-20