Molecular analysis for chromatin structure conserved beyond generations
Project/Area Number |
22870038
|
Research Category |
Grant-in-Aid for Research Activity Start-up
|
Allocation Type | Single-year Grants |
Research Field |
Genetics/Genome dynamics
|
Research Institution | The Institute of Physical and Chemical Research |
Principal Investigator |
YOSHIDA Keisuke 独立行政法人理化学研究所, 石井分子遺伝学研究室, 基礎科学特別研究員 (80587452)
|
Project Period (FY) |
2010 – 2011
|
Project Status |
Completed (Fiscal Year 2011)
|
Budget Amount *help |
¥3,146,000 (Direct Cost: ¥2,420,000、Indirect Cost: ¥726,000)
Fiscal Year 2011: ¥1,508,000 (Direct Cost: ¥1,160,000、Indirect Cost: ¥348,000)
Fiscal Year 2010: ¥1,638,000 (Direct Cost: ¥1,260,000、Indirect Cost: ¥378,000)
|
Keywords | 次世代遺伝効果 / 自然免疫系 / マクロファージ / ATF-7 / 自然免疫 / ATF7 |
Research Abstract |
ATF-7 is reported to contribute to transgenerational effect of heat shock stress in fly. This time, I investigated molecular function of ATF-7 in innate immunity system using resident peritoneal macrophages. ChIP-chip results with ATF-7 antibody showed ATF-7 binds to promoter region of several tens of innate immunityrelated genes. At these sites, H3K9me2 level decrease and gene activity is enhanced in ATF-7 KO. I found that after treating macrophages by innate immune stress, ATF-7 binding and H3K9me2 level is decreased.
|
Report
(3 results)
Research Products
(1 results)