Molecular analysis for chromatin structure conserved beyond generations

Research Project

Project/Area Number	22870038
Research Category	Grant-in-Aid for Research Activity Start-up
Allocation Type	Single-year Grants
Research Field	Genetics/Genome dynamics
Research Institution	The Institute of Physical and Chemical Research
Principal Investigator	YOSHIDA Keisuke 独立行政法人理化学研究所, 石井分子遺伝学研究室, 基礎科学特別研究員 (80587452)
Project Period (FY)	2010 – 2011
Project Status	Completed (Fiscal Year 2011)
Budget Amount *help	¥3,146,000 (Direct Cost: ¥2,420,000、Indirect Cost: ¥726,000) Fiscal Year 2011: ¥1,508,000 (Direct Cost: ¥1,160,000、Indirect Cost: ¥348,000) Fiscal Year 2010: ¥1,638,000 (Direct Cost: ¥1,260,000、Indirect Cost: ¥378,000)
Keywords	次世代遺伝効果 / 自然免疫系 / マクロファージ / ATF-7 / 自然免疫 / ATF7
Research Abstract	ATF-7 is reported to contribute to transgenerational effect of heat shock stress in fly. This time, I investigated molecular function of ATF-7 in innate immunity system using resident peritoneal macrophages. ChIP-chip results with ATF-7 antibody showed ATF-7 binds to promoter region of several tens of innate immunityrelated genes. At these sites, H3K9me2 level decrease and gene activity is enhanced in ATF-7 KO. I found that after treating macrophages by innate immune stress, ATF-7 binding and H3K9me2 level is decreased.