Elucidation of the regulating mechanism of glycolipid synthesis by caveolin

• Project/Area Number: 22870041
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Molecular biology
• Research Institution: The Institute of Physical and Chemical Research
• Principal Investigator: ISHIBASHI Yohei 独立行政法人理化学研究所, 神経膜機能研究チーム, 基礎科学特別研究員 (90572868)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥2,717,000 (Direct Cost: ¥2,090,000、Indirect Cost: ¥627,000); Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2010: ¥1,417,000 (Direct Cost: ¥1,090,000、Indirect Cost: ¥327,000)
• Keywords: スフィンゴ脂質 / 糖脂質 / グルコシルセラミド / AMPK / エネルギー代謝 / UDP-グルコース / スフィンゴ糖脂質 / カベオリン

Research Abstract

We hypothesized that caveolin may regulate the GlcCer synthase activity. We developed a cell-based assay of GlcCer synthase activity to verify our hypothesis. This assay revealed that caveolin is probably not a regulator of GlcCer synthase. We discovered that the energy sensor AMPK regulates the cellular GlcCer level.

Research Products

• [Journal Article] Quality Control of Fungus-specific Gluco sylceramide in Cryptococcus neoformans by En doglycoceramidase-related Protein 1(EGCrP1) (2012)
  • Author(s): Yohei Ishibashi, Kazutaka Ikeda, Keishi Sa kaguchi, Nozomu Okino, Ryo Taguchi, Makoto Ito
  • Journal Title: J. Biol. Chem. Volume: 287 Pages: 368-381
  • Peer Reviewed
• [Journal Article] Quality control of fungus-specific glucosylceraraide in Cryptococcus neoformans by endoglycoceramidase-related protein 1 (EGCrP1) (2012)
  • Author(s): Y.Ishibashi, et al
  • Journal Title: Journal of Biological Chemistry Volume: 287 Issue: 1 Pages: 368-381
  • DOI: 10.1074/jbc.m111.311340
  • Peer Reviewed
• [Journal Article] Qualitative and quantitative cellular glycomics of glycosphingolipids based on rhodococcal endoglycosylceramidase-assisted glycan cleavage, glycoblotting-assisted sample preparation, and matrix-assisted laser desorption ionization tandem time-of-flight mass spectrometry analysis (2011)
  • Author(s): Naoki Fujitani, Yasuhiro Takegawa, Yohei Ishibashi, Kayo Araki, Jun-ichi Furukawa, Susumu Mitsutake, Yasuyuki Igarashi, Makoto Ito, Yasuro Shinohara
  • Journal Title: J. Biol. Chem. Volume: 286 Pages: 1669-1679
  • Peer Reviewed
• [Journal Article] QualitativeandQuantitative Cellular Glycomics of Glycosphingolipids Based on Rhodococcal Endoglycosylceramidase-assisted Glycan Cleavage, Glycoblotting-assisted Sample Preparation, and Matrix-assisted Laser Desorption Ionization Tandem Time-of-flight Mass Spectrometry Analysis (2011)
  • Author(s): Naoki Fujitani, Yasuhiro Takegawa, Yohei Ishibashi, Kayo Araki, Jun-ichi Furukawa, Susumu Mitsutake, Yasuyuki Igarashi, Makoto Ito, Yasuro Shinohara
  • Journal Title: THE JOURNAL OF BIOLOGICAL CHEMISTRY Volume: 286 Issue: 48 Pages: 41669-41679
  • DOI: 10.1074/jbc.m111.301796
  • Peer Reviewed
• [Presentation] 真菌より見出されたユニークな糖脂質代謝機構 (2012)
  • Author(s): 石橋洋平
  • Organizer: 生命応答を制御する脂質マシナリー若手ワークショップ
  • Place of Presentation: 東京大学(東京都)
• [Presentation] 細胞内エネルギー状態に応じたグルコシルセラミド量調節因子の解明 (2011)
  • Author(s): 石橋洋平
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜(横浜市)
• [Presentation] 細胞内エネルギーセンサーAMPKによるグルコシルセラミド合成制御機構の解明 (2011)
  • Author(s): 石橋洋平
  • Organizer: 第4回セラミド研究会学術集会
  • Place of Presentation: 北海道大学(札幌市)
• [Presentation] AMP-activated protein kinase negatively regulates Glucosylceramide synthesis in Mouse fibroblast (2011)
  • Author(s): 石橋洋平
  • Organizer: 第30回内藤コンファレンス
  • Place of Presentation: シャトレーゼガトーキングダムサッポロ(札幌市)
• [Presentation] AMP-activated protein kinase negatively regulates Glucosylceramide synthesis in Mouse fibroblast (2011)
  • Author(s): Yohei Ishibashi, Yoshio Hirabayashi
  • Organizer: 第30回内藤コンファレンス
  • Place of Presentation: シャトレーゼ ガトーキングダム サッポロ(札幌市)
• [Presentation] 真菌より見出されたユニークな糖脂質代謝機構 (2011)
  • Author(s): 石橋洋平
  • Organizer: 生命応答を逝去する脂質マシナリー若手ワークショップ
  • Place of Presentation: 東京大学(東京都)
• [Presentation] 新規糖質加水分解酵素EGCrPは真菌糖脂質の代謝に関与する (2010)
  • Author(s): 石橋洋平, (他4名)
  • Organizer: 第34回九州シンポジウム
  • Place of Presentation: 福岡県宗像市
  • Year and Date: 2010-09-09
• [Remarks] Yohei Ishibashi, Fluorescent labeling of 6-gala(neogala) series glycosphingolipids by EGALC, GlycoScience Protocol Online Database(GlycoPOD)実験プロトコル執筆(日本糖鎖科学統合データベース)
• [Remarks] Yohei Ishibashi, Degradation of Glycolipids by Endoglycoceramidase, GlycoScience Protocol Online Database(GlycoPOD)実験プロトコル執筆(日本糖鎖科学統合データベース)
• [Remarks]
  • URL: http://www.kyushu-u.ac.jp/pressrelease/2011/2011_11_15.pdf