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Role of nutrient sensor AMPK on regulation of myogenic inhibitory factor myostatin.

Research Project

Project/Area Number 22880006
Research Category

Grant-in-Aid for Research Activity Start-up

Allocation TypeSingle-year Grants
Research Field Applied animal science
Research InstitutionThe University of Tokushima (2011)
Tohoku University (2010)

Principal Investigator

MIYAKE Masato  徳島大学, 疾患ゲノム研究センター, 特任助教 (30588976)

Project Period (FY) 2010 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥3,146,000 (Direct Cost: ¥2,420,000、Indirect Cost: ¥726,000)
Fiscal Year 2011: ¥1,508,000 (Direct Cost: ¥1,160,000、Indirect Cost: ¥348,000)
Fiscal Year 2010: ¥1,638,000 (Direct Cost: ¥1,260,000、Indirect Cost: ¥378,000)
Keywordsウシ / 骨格筋 / マイオスタチン / AMPK / グルコース / 分化 / 筋芽細胞 / 初代培養 / シグナル伝達 / Smad / 成長
Research Abstract

Primary myoblasts that can efficiently differentiate to myotube was established from bovine skeletal muscle. The myoblasts was expressed myoblast marker Myf5 during proliferation and differentiation marker myogenin during differentiation. These cells were inhibited differentiation and MSTN expression under low glucose condition, which activates AMPK. However expression of MSTN receptor was not changed. AMPK activator AICAR also inhibited differentiation and expression of MSTN in myotubes. The effect of antagonist of MSTN signaling was abolished by AICAR. These results suggest that AMPK regulates the action of MSTN.

Report

(3 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • Research Products

    (17 results)

All 2012 2011 2010 Other

All Journal Article (5 results) (of which Peer Reviewed: 5 results) Presentation (11 results) Remarks (1 results)

  • [Journal Article] AMPK activation by AICAR inhibits myogenic differentiation and myostatin expression in cattle.2012

    • Author(s)
      Masato Miyake
    • Journal Title

      Cell and Tissue Research

      Volume: 349 Issue: 2 Pages: 615-623

    • DOI

      10.1007/s00441-012-1422-8

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] TIEG1 negatively controls the myoblast pool indispensable for fusion during myogenic differentiation of C2C12 cells2011

    • Author(s)
      Masato Miyake, Shinichiro Hayashi, Shunsuke Iwasaki, Takafumi Uchida, Kouichi Watanabe, Shyuichi Ohwada, Hisashi Aso, Takahiro Yamaguchi.
    • Journal Title

      Journal of Cellular Physiology

      Volume: 226 Issue: 4 Pages: 1128-1136

    • DOI

      10.1002/jcp.22434

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] TIEGI negatively controls the myoblast pool indispensable for fusion during myogenic differentiation of C2C12 cells.2011

    • Author(s)
      Miyake M, et al.
    • Journal Title

      Journal of Cellular Physiology

      Volume: 226 Pages: 1128-1136

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Peripheral Serotonin Enhances Lipid Metabolism by Accelerating Bile Acid Turnover2010

    • Author(s)
      Hitoshi Watanabe, Daisuke Akasaka, Hideki Ogasawara, Kan Sato, Masato Miyake, Hisashi Aso et al.
    • Journal Title

      Endocrinology

      Volume: 151 Issue: 10 Pages: 4776-4786

    • DOI

      10.1210/en.2009-1349

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Peripheral serotonin enhances lipid metabolism by accelerating bile acidturnover.2010

    • Author(s)
      Watanabe H, et al.
    • Journal Title

      Endocrinology

      Volume: 151 Pages: 4776-4786

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Presentation] ウシ骨格筋筋線維型に依存した筋衛星細胞のミオスタチン発現2012

    • Author(s)
      北川絵理、三宅雅人、高橋秀之、櫻田隆大、白須直樹、渡邊康一、大和田修一、麻生久
    • Organizer
      第115回日本畜産学会大会
    • Place of Presentation
      古屋大学(名古屋市)
    • Year and Date
      2012-03-27
    • Related Report
      2011 Final Research Report
  • [Presentation] 草原短角種牛におけるグルコース代謝特性2012

    • Author(s)
      麻生久、高橋秀之、三宅雅人、浦川めぐみ、渡邉一史、長澤裕哉、寺田俊介、大崎慶也、平田利幸、佐藤光美、佐藤富雄、大和田修一、渡邊康一
    • Organizer
      第115回日本畜産学会大会
    • Place of Presentation
      名古屋大学(名古屋市)
    • Year and Date
      2012-03-27
    • Related Report
      2011 Final Research Report
  • [Presentation] ウシ骨格筋線維型における筋小胞体カルシウムイオンポンプの発現様式2011

    • Author(s)
      櫻田隆大、白須直樹、北川絵理、三宅雅人、大和田修一、山口高弘、麻生久、渡邊康一
    • Organizer
      第61回東北畜産学会大会
    • Place of Presentation
      青森市文化観光交流施設(青森市)
    • Year and Date
      2011-09-08
    • Related Report
      2011 Final Research Report
  • [Presentation] 放牧など自給粗飼料100%で生産した日本短角種および北里八雲牛の枝肉成績と筋線維型構成2011

    • Author(s)
      小笠原英毅、畔柳正、米澤智洋、渡邊康一、三宅雅人、高橋秀之、小野泰、大和田修一、麻生久、山口高弘、寳示戸雅之
    • Organizer
      第114回日本畜産学会大会
    • Place of Presentation
      北里大学(十和田市)
    • Year and Date
      2011-08-26
    • Related Report
      2011 Final Research Report
  • [Presentation] 日本短角種ならびにホルスタイン種子ウシの白血球とT細胞サブセットの比較2011

    • Author(s)
      浦川めぐみ、名谷真琴、高橋秀之、三宅雅人、堀越頼子、佐藤富男、佐藤光美、大崎慶也、平田利幸、麻生久、山口高弘、黒毛和種
    • Organizer
      第114回日本畜産学会大会
    • Place of Presentation
      北里大学(十和田市)
    • Year and Date
      2011-08-26
    • Related Report
      2011 Final Research Report
  • [Presentation] Early adaptation of sarcoplasmic reticulum Ca2+ pump in bovine myofiber under chronic low-frequency electrical stimulation2011

    • Author(s)
      Takahiro Sakurada, Eri Kitagawa, Masato Miyake, Shyuichi Ohwada, Hisashi Aso, Kouichi Watanabe.
    • Organizer
      ADSA-ASAS 2011 Joint Annual Meeting
    • Place of Presentation
      Ernest N. Morial Convention Center(アメリカ合衆国ルイジアナ州)
    • Year and Date
      2011-07-10
    • Related Report
      2011 Final Research Report
  • [Presentation] 黒毛和種新生仔牛の末梢血における白血球およびT細胞サブセットの出現割合2010

    • Author(s)
      名谷真琴、高橋秀之、三宅雅人、堀越頼子、渡邊康一、田中沙智、佐藤富雄、佐藤光美、大崎慶也、平田利幸、大和田修一、麻生久、山口高弘
    • Organizer
      第60回東北畜産学会大会
    • Place of Presentation
      いわて県民情報交流センター(盛岡市)
    • Year and Date
      2010-08-29
    • Related Report
      2011 Final Research Report
  • [Presentation] 血小板由来セロトニンの骨格筋再生に及ぼす影響2010

    • Author(s)
      巣国正、三宅雅人、渡邉一史、北川絵理、中野辰也、高橋遊、大和田修一、渡邊康一、麻生久、山口高弘
    • Organizer
      第60回東北畜産学会大会
    • Place of Presentation
      いわて県民情報交流センター(盛岡市)
    • Year and Date
      2010-08-29
    • Related Report
      2011 Final Research Report
  • [Presentation] 牛骨格筋における筋衛星細胞の分布と挙動2010

    • Author(s)
      北川絵理、三宅雅人、今井由佳、岩崎俊輔、渡邊康一、大和田修一、麻生久、山口高弘
    • Organizer
      第60回東北畜産学会大会
    • Place of Presentation
      いわて県民情報交流センター(盛岡市)
    • Year and Date
      2010-08-29
    • Related Report
      2011 Final Research Report
  • [Presentation] 糖代謝および脂質代謝における末梢セロトニンの作用機構の解析2010

    • Author(s)
      渡邉一史、齋藤和輝、三宅雅人、高橋遊、本堂哲也、佐藤幹、大和田修一、渡邊康一、山口高弘、麻生久
    • Organizer
      第60回東北畜産学会大会
    • Place of Presentation
      いわて県民情報交流センター(盛岡市)
    • Year and Date
      2010-08-29
    • Related Report
      2011 Final Research Report
  • [Presentation] 末梢セロトニンの血糖値上昇作用メカニズムの解析2010

    • Author(s)
      渡邉一史、齋藤和輝、三宅雅人、高橋遊、本堂哲也、佐藤幹、大和田修一、渡邊康一、山口高弘、麻生久
    • Organizer
      第15回アディポサイエンス研究会
    • Place of Presentation
      千里阪急ホテル(豊中市)
    • Year and Date
      2010-08-21
    • Related Report
      2011 Final Research Report
  • [Remarks]

    • URL

      http://www.agri.tohoku.ac.jp/keitai/index-j.html

    • Related Report
      2010 Annual Research Report

URL: 

Published: 2010-08-27   Modified: 2016-04-21  

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