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DNA barcoding to detect putative hybrid species in bamboos

Research Project

Project/Area Number 22880010
Research Category

Grant-in-Aid for Research Activity Start-up

Allocation TypeSingle-year Grants
Research Field Forest science
Research InstitutionThe University of Tokyo

Principal Investigator

HISAMOTO Yoko  東京大学, 大学院・農学生命科学研究科, 助教 (60586014)

Project Period (FY) 2010 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥3,146,000 (Direct Cost: ¥2,420,000、Indirect Cost: ¥726,000)
Fiscal Year 2011: ¥1,508,000 (Direct Cost: ¥1,160,000、Indirect Cost: ¥348,000)
Fiscal Year 2010: ¥1,638,000 (Direct Cost: ¥1,260,000、Indirect Cost: ¥378,000)
Keywordsササ類 / 推定雑種分類群 / 核遺伝子マーカー / 葉緑体遺伝子マーカー / 花成促進遺伝子FLOWERING LOCUS T(FT) / タケ亜科植物 / DNAバーコーディング / 花成促進遺伝子 / 花芽運命決定遺伝子 / 雑種検出 / 花成遺伝子
Research Abstract

Japanese dwarf bamboos(Sasa-group) develop hybrids easily, and the identification on the basis of the external morphologic traits of vegetative organs is difficult. The aim of this study was to investigate the usefulness of chloroplast and nuclear genes as DNA markers for differentiation between Sasa-group hybrid taxa. Thirty-seven and sixteen samples were collected in the University of Tokyo Chichibu Forest and the University of Tokyo Hokkaido Forest, respectively. Intergeneric and interspecific hybrids were identified on the basis of external morphologic traits. At the result of molecular analysis, one of the flowering genes, FLOWERING LOCUS T(FT) may be a useful DNA marker to detect hybrids.

Report

(3 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • Research Products

    (5 results)

All 2012 2010

All Journal Article (3 results) (of which Peer Reviewed: 3 results) Presentation (2 results)

  • [Journal Article] Flowering habit of two bamboos Phyllostachys meyeri and Shibataea chinensis analyzed with flowering gene expression2012

    • Author(s)
      Hisamoto, Y. and Kobayashi, M.
    • Journal Title

      Plant Species Biology

      Volume: 27(2) Issue: 2 Pages: 109-117

    • DOI

      10.1111/j.1442-1984.2012.00369.x

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Protoplast isolation from bamboo leaves2010

    • Author(s)
      Hisamoto Y., Kobayashi M
    • Journal Title

      Plant Biotechnology

      Volume: 27 Pages: 353-358

    • NAID

      10026706110

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Protoplast isolation from bamboo leaves.2010

    • Author(s)
      Hisamoto Y., Kobayashi M.
    • Journal Title

      Plant Biotechnology

      Volume: 27 Pages: 353-358

    • NAID

      10026706110

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Presentation] 日本産ササ類における推定雑種検出を可能とする開花遺伝子マーカーの検討2012

    • Author(s)
      久本洋子
    • Organizer
      第123回日本森林学会
    • Place of Presentation
      宇都宮大学峰キャンパス(栃木県)
    • Year and Date
      2012-03-28
    • Related Report
      2011 Annual Research Report 2011 Final Research Report
  • [Presentation] Utility of flowering genes as nuclear DNA markers to detect putative hybrid species in Japanese dwarf bamboos2012

    • Author(s)
      Hisamoto Y
    • Organizer
      第59回日本生態学会
    • Place of Presentation
      龍谷大学瀬田キャンパス(滋賀県)
    • Year and Date
      2012-03-18
    • Related Report
      2011 Annual Research Report 2011 Final Research Report

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Published: 2010-08-27   Modified: 2016-04-21  

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