Mechanism of high-density lipoprotein(HDL) formation by ABCA1

• Project/Area Number: 22880016
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Applied biochemistry
• Research Institution: Kyoto University
• Principal Investigator: NAGAO Kohjiro 京都大学, 物質-細胞統合システム拠点, 研究員 (40587325)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥3,146,000 (Direct Cost: ¥2,420,000、Indirect Cost: ¥726,000); Fiscal Year 2011: ¥1,508,000 (Direct Cost: ¥1,160,000、Indirect Cost: ¥348,000); Fiscal Year 2010: ¥1,638,000 (Direct Cost: ¥1,260,000、Indirect Cost: ¥378,000)
• Keywords: cholesterol / ABCA1 / HDL

Research Abstract

ABCA1 mediates the high-density lipoprotein(HDL) formation by loading cellular cholesterol onto apolipoproteinA-I(apoA-I). In this study, we analyzed the interaction between ABCA1 and apoA-I, which is first step of HDL formation. We showed that conformation of extracellular domain of ABCA1 is changed by ATP hydrolysis in nucleotide domain, and apoA-I binding site is produced by this conformation changes.

Research Products

• [Journal Article] ATP hydrolysis-dependent conformational changes in the extracellular domain of ABCA1 are associated with apoA-I binding. (2012)
  • Author(s): Nagao, K., Takahashi, K., Azuma, Y., Takada, M., Kimura, Y., Matsuo, M., Kioka, N. and Ueda, K.
  • Journal Title: J Lipid Res Volume: 53 Issue: 1 Pages: 126-136
  • DOI: 10.1194/jlr.m019976
  • Peer Reviewed
• [Journal Article] Lysine residues of ABCA1 are required for the interaction with apoA-I (2012)
  • Author(s): Nagao, K.
  • Journal Title: Biochin Biophys Ada Volume: 1,821 Issue: 3 Pages: 530-535
  • DOI: 10.1016/j.bbalip.2011.06.024
  • Peer Reviewed
• [Journal Article] Function and regulation of ABCA1-Membrane meso-domain organization and re-organizationprotrusions (2011)
  • Author(s): Nagao, K.
  • Journal Title: FEBS J. Volume: 278 Issue: 18 Pages: 3190-3203
  • DOI: 10.1111/j.1742-4658.2011.08170.x
  • Peer Reviewed
• [Journal Article] Function and regulation of ABCA1- Membrane meso-domain organization and re-organization (2011)
  • Author(s): Kohjiro Nagao, Maiko Tomioka, Kazumitsu Ueda
  • Journal Title: FEBS Journal Volume: (印刷中)
  • Peer Reviewed
• [Journal Article] Lipid outward translocation by ABC proteins (2010)
  • Author(s): Nagao, K., Kimura, Y., Matsuo, M. and Ueda, K
  • Journal Title: FEBS Lett Volume: 584 Issue: 13 Pages: 2717-2723
  • DOI: 10.1016/j.febslet.2010.04.036
  • Peer Reviewed
• [Journal Article] Lipid outward translocation by ABC proteins (2010)
  • Author(s): Kohjiro Nagao, Yasuhisa Kimura, Michinori Mastuo, Kazumitsu Ueda
  • Journal Title: FEBS letters Volume: 584 Pages: 2717-2723
  • NAID: 120002484960
  • Peer Reviewed
• [Presentation] Cyclosporine AによるABCA1の阻害機構の解析 (2012)
  • Author(s): 長尾耕治郎, 前田南, 植田和光
  • Organizer: 日本農芸化学会2012年度大会
  • Place of Presentation: 京都女子大学
• [Presentation] ATP hydrolysis-dependent conformational changes in the extracellular domain of ABCA1 are associated with apoA-I binding ABC2012 (2012)
  • Author(s): Kohjiro Nagao, Yasuhisa Kimura, Michinori Matsuo and Kazumitsu Ueda
  • Organizer: ABC2012
  • Place of Presentation: Innsbruck, Austria
• [Presentation] Cyclosporine AによるABCA1の阻害機構の解析 (2012)
  • Author(s): 長尾耕治郎, 前田南, 植田和光
  • Organizer: 日本農芸化学会2012年度大会
  • Place of Presentation: 京都女子大学(京都府)
• [Presentation] ATP hydrolysis-dependent conformational changes in the extracellular domain of ABCA1 are associated with apoA-I binding (2012)
  • Author(s): Kohjiro Nagao, Yasuhisa Kimura, Michinori Matsuo, Kazumitsu Ueda
  • Organizer: ABC2012
  • Place of Presentation: Innsbruck, Austria
• [Presentation] ATP加水分解に依存したABCA1の細胞外領域の構造変化 (2011)
  • Author(s): 長尾耕治郎、木村泰久、植田和光
  • Organizer: 日本農芸化学会2011年度大会
  • Place of Presentation: 京都府、京都女子大学
  • Year and Date: 2011-03-27
• [Presentation] 高密度リポタンパク質(HDL)産生機構の解析(1)ABCA1のATPに依存した構造変化とapoA-I結合 (2011)
  • Author(s): 長尾耕治郎, 木村泰久, 松尾道憲, 木岡紀幸, 植田和光
  • Organizer: 2011年度日本農芸化学会関西・中部支部合同大会
  • Place of Presentation: 京都大学
• [Presentation] ATP加水分解に依存したABCA1の細胞外領域の構造変化がapoA-Iとの結合に必須である (2011)
  • Author(s): 長尾耕治郎、高橋圭、吾妻佑哉、木村泰久、松尾道憲、木岡紀幸、植田和光
  • Organizer: 第84回日本生化学会
  • Place of Presentation: 国立京都国際会館
• [Presentation] ATP加水分解に依存したABCA1の細胞外領域の構造変化 (2011)
  • Author(s): 長尾耕治郎、木村泰久、植田和光
  • Organizer: 日本農芸化学会2011年度大会
  • Place of Presentation: 京都女子大学
• [Presentation] ATP加水分解に依存したABCA1の細胞外領域の構造変化がapoA-Iとの結合に必須である (2011)
  • Author(s): 長尾耕治郎、高橋圭、吾妻佑哉、木村泰久、松尾道憲、木岡紀幸、植田和光
  • Organizer: 第84回日本生化学会
  • Place of Presentation: 国立京都国際会館(京都府)
• [Presentation] 高密度リポタンパク質(HDL)産生機構の解析(1)ABCA1のATPに依存した構造変化とapoA-I結合 (2011)
  • Author(s): 長尾耕治郎, 木村泰久, 松尾道憲, 木岡紀幸, 植田和光
  • Organizer: 2011年度日本農芸化学会関西・中部支部合同大会
  • Place of Presentation: 京都大学(京都府)