| Project/Area Number |
22880033
|
|---|
| Research Category |
Grant-in-Aid for Research Activity Start-up
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
Bioproduction chemistry/Bioorganic chemistry
|
|---|
| Research Institution | Tokai University |
|---|
Principal Investigator |
|
|---|
| Project Period (FY) |
2010 – 2011
|
| Project Status |
Completed (Fiscal Year 2011)
|
| Budget Amount *help |
¥3,042,000 (Direct Cost: ¥2,340,000、Indirect Cost: ¥702,000)
Fiscal Year 2011: ¥1,404,000 (Direct Cost: ¥1,080,000、Indirect Cost: ¥324,000)
Fiscal Year 2010: ¥1,638,000 (Direct Cost: ¥1,260,000、Indirect Cost: ¥378,000)
|
|---|
| Keywords | 有機化学 / ペプチド化学 / 糖タンパク質 / 糖ペプチド / S-結合型糖鎖 / Py1RS / sublancin / チオエステル / Pocam基 / チオエステル法 / Mercaptomethyl基 / セグメント縮合反応 / PylRS |
|---|
| Research Abstract |
In this study, we tried to develop a novel thiol protecting group which was useful for protecting Cys side chain. As a result, Pocam group could be designed, synthesized and used for solid-phase peptide synthesis and peptide condensation reactions. On the other hand, we tried to synthesize neo-glycoproteins by the combination of bacterial recombinant protein expression system and in vitro glycosylation reaction. As a result, we could prepare a recombinant protein containing a specific functional group. A glycopeptide carrying S-linked glycan which was recently found as a novel post-translational modification was chemically synthesized.
|
