Development of a new method for estimating fine root biomass from the quantity of DNA of target plant
| Project/Area Number |
22880040
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Forest science
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| Research Institution | Forestry and Forest Products Research Institute |
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Principal Investigator |
UGAWA Shin 独立行政法人森林総合研究所, 立地環境研究領域, 研究員 (30582738)
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| Project Period (FY) |
2010 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥2,223,000 (Direct Cost: ¥1,710,000、Indirect Cost: ¥513,000)
Fiscal Year 2011: ¥767,000 (Direct Cost: ¥590,000、Indirect Cost: ¥177,000)
Fiscal Year 2010: ¥1,456,000 (Direct Cost: ¥1,120,000、Indirect Cost: ¥336,000)
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| Keywords | 細根バイオマス量 / リアルタイムPCR / DNA抽出効率 / スキムミルク / アロフェン / 単回帰分析 / 推定誤差 |
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| Research Abstract |
The practical use of a new method for estimating fine root biomass of target plant from the quantity of DNA extracted from fine root mixed with soil particles was examined. A DNA extraction method was improved to obtain the optimal level of DNA extraction efficiency for Zea mays fine root mixed with Kanuma soil, which adsorbs DNA strongly. Using the improved DNA extraction method, a regression line between the amount of extracted DNA and fine root biomass could be obtained. Moreover, the estimation error in the method did not differ among the fraction sizes of fine roots.
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Report
(3 results)
Research Products
(1 results)
