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Development of a new method for estimating fine root biomass from the quantity of DNA of target plant

Research Project

Project/Area Number 22880040
Research Category

Grant-in-Aid for Research Activity Start-up

Allocation TypeSingle-year Grants
Research Field Forest science
Research InstitutionForestry and Forest Products Research Institute

Principal Investigator

UGAWA Shin  独立行政法人森林総合研究所, 立地環境研究領域, 研究員 (30582738)

Project Period (FY) 2010 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥2,223,000 (Direct Cost: ¥1,710,000、Indirect Cost: ¥513,000)
Fiscal Year 2011: ¥767,000 (Direct Cost: ¥590,000、Indirect Cost: ¥177,000)
Fiscal Year 2010: ¥1,456,000 (Direct Cost: ¥1,120,000、Indirect Cost: ¥336,000)
Keywords細根バイオマス量 / リアルタイムPCR / DNA抽出効率 / スキムミルク / アロフェン / 単回帰分析 / 推定誤差
Research Abstract

The practical use of a new method for estimating fine root biomass of target plant from the quantity of DNA extracted from fine root mixed with soil particles was examined. A DNA extraction method was improved to obtain the optimal level of DNA extraction efficiency for Zea mays fine root mixed with Kanuma soil, which adsorbs DNA strongly. Using the improved DNA extraction method, a regression line between the amount of extracted DNA and fine root biomass could be obtained. Moreover, the estimation error in the method did not differ among the fraction sizes of fine roots.

Report

(3 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • Research Products

    (1 results)

All 2012

All Presentation (1 results)

  • [Presentation] DNA定量法を用いた細根バイオマス量の測定手法の開発2012

    • Author(s)
      鵜川信、山口宗義、三浦覚、金子真司
    • Organizer
      日本森林学会
    • Place of Presentation
      宇都宮大学(栃木県)
    • Year and Date
      2012-03-27
    • Related Report
      2011 Annual Research Report 2011 Final Research Report

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Published: 2010-08-27   Modified: 2016-04-21  

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