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Structure analyses of dextran synthases

Research Project

Project/Area Number 22880043
Research Category

Grant-in-Aid for Research Activity Start-up

Allocation TypeSingle-year Grants
Research Field Applied biochemistry
Research InstitutionHiroshima University

Principal Investigator

HONDOH Hironori  広島大学, 大学院・生物圏科学研究科, 講師 (10368003)

Project Period (FY) 2010 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥2,639,000 (Direct Cost: ¥2,030,000、Indirect Cost: ¥609,000)
Fiscal Year 2011: ¥1,157,000 (Direct Cost: ¥890,000、Indirect Cost: ¥267,000)
Fiscal Year 2010: ¥1,482,000 (Direct Cost: ¥1,140,000、Indirect Cost: ¥342,000)
Keywords糖質関連酵素 / 基質特異性 / デキストラングルコシダーゼ / I6GT / DexB / デキストラン / 糖質合成酵素 / X線結晶構造解析
Research Abstract

We aimed to reveal the substrate recognition mechanism of isomaltooligosaccharide synthases and hydrolases with their crystal structures. With the crystal structure of DGase, isolamtooligosaccharide hydrolase, it is proposed that the water drain structure at the bottom of the active pocket. We study the effect of the site-directed mutagenesis in this water drain on the hydrolysis activity of this enzyme. The hydrolysis activity was decreased significantly without any difference in its structure.

Report

(3 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • Research Products

    (16 results)

All 2011 2010

All Journal Article (2 results) (of which Peer Reviewed: 2 results) Presentation (12 results) Book (2 results)

  • [Journal Article] Calcium Ion-Dependent Increase in Thermostability of Dextran Glucosidase from Streptococcus mutans2011

    • Author(s)
      Kobayashi M
    • Journal Title

      Bioscience, Biotechnology, and Biochemistry

      Volume: 75 Issue: 8 Pages: 1557-1563

    • DOI

      10.1271/bbb.110256

    • NAID

      10029591699

    • ISSN
      0916-8451, 1347-6947
    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Effects of temperature2011

    • Author(s)
      Yoshihisa Suzuki, Emi Konda, Hironori Hondoh, Katsuhiro Tamura
    • Journal Title

      pressure, and pH on the solubility of triclinic lysozyme crystals

      Volume: 318巻 Issue: 1 Pages: 1085-1088

    • DOI

      10.1016/j.jcrysgro.2010.11.107

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Presentation] 乳脂肪高融点画分の球晶の解明2011

    • Author(s)
      板谷修二
    • Organizer
      日本結晶成長学会
    • Place of Presentation
      つくば国際会議場
    • Year and Date
      2011-11-05
    • Related Report
      2011 Annual Research Report
  • [Presentation] ココアバター結晶化におけるせん断応力と添加物の影響2011

    • Author(s)
      森行和哉
    • Organizer
      日本結晶成長学会
    • Place of Presentation
      つくば国際会議場
    • Year and Date
      2011-11-04
    • Related Report
      2011 Annual Research Report
  • [Presentation] O/Wエマルション中の脂溶性物質の可溶化状態と結晶化抑制2011

    • Author(s)
      山崎健太
    • Organizer
      日本結晶成長学会
    • Place of Presentation
      つくば国際会議場
    • Year and Date
      2011-11-04
    • Related Report
      2011 Annual Research Report
  • [Presentation] Isomaltooligosaccharide 6-α-glucosyltransferaseサブサイト+ 1変異酵素の機能解析2011

    • Author(s)
      青山泰, 西村崇志, 鐘ケ江倫世, 本同宏成, 奥山正幸, 森春英, 木村淳夫,
    • Organizer
      日本応用糖質科学会平成23年度大会
    • Place of Presentation
      北海道大学
    • Year and Date
      2011-09-28
    • Related Report
      2011 Final Research Report
  • [Presentation] Dextran glucosidase(DexB)ウォーターパス改変体の機能と構造,2011

    • Author(s)
      小林桃子, 山下恵太郎, 田上貴祥, 本同宏成, 森春英, 奥山正幸, 姚閔, 木村淳夫,
    • Organizer
      日本応用糖質科学会平成23年度大会
    • Place of Presentation
      北海道大学
    • Year and Date
      2011-09-28
    • Related Report
      2011 Final Research Report
  • [Presentation] Isomaltooligosaccharide 6-α-glucosyltransferaseサブサイト+1変異酵素の機能解析2011

    • Author(s)
      青山泰
    • Organizer
      日本応用糖質科学会
    • Place of Presentation
      北海道大学
    • Year and Date
      2011-09-28
    • Related Report
      2011 Annual Research Report
  • [Presentation] Dextran glucosidase (DexB)クォーターパス改変体の機能と構造2011

    • Author(s)
      少林桃子
    • Organizer
      日本応用糖質科学会
    • Place of Presentation
      北海道大学
    • Year and Date
      2011-09-28
    • Related Report
      2011 Annual Research Report
  • [Presentation] 低濃度ファットスプレッドにおIけるモノアシルグリセロール乳化剤が油脂結晶化に与える影響2011

    • Author(s)
      岡村愛里
    • Organizer
      日本食品科学工学会
    • Place of Presentation
      東北大学
    • Year and Date
      2011-09-10
    • Related Report
      2011 Annual Research Report
  • [Presentation] 乳脂高融点画分の結晶化の解明2011

    • Author(s)
      板谷修二
    • Organizer
      日本食品科学工学会
    • Place of Presentation
      東北大学
    • Year and Date
      2011-09-10
    • Related Report
      2011 Annual Research Report
  • [Presentation] Dextran glucosidase(DexB)のウォーターパス改変酵素の機能解析2011

    • Author(s)
      小林桃子、本同宏成、奥山正幸、森春英、木村淳夫
    • Organizer
      日本農芸化学会2011年度大会
    • Place of Presentation
      京都女子大
    • Year and Date
      2011-03-26
    • Related Report
      2011 Final Research Report 2010 Annual Research Report
  • [Presentation] Isomaltooligosaccharide 6-α-glucosyltransferaseのサブサイト+ 1変異酵素の機能解析2010

    • Author(s)
      西村崇志, 鐘ケ江倫世, 本同宏成, 奥山正幸, 森春英, 木村淳夫,
    • Organizer
      日本応用糖質科学会平成22年度大会
    • Place of Presentation
      静岡県コンベンションアーツセンター
    • Year and Date
      2010-09-15
    • Related Report
      2011 Final Research Report
  • [Presentation] Isomaltooligosaccharide 6-a-glucosyltransferaseのサブサイト+1変異酵素の機能解析2010

    • Author(s)
      西村崇志, 鐘ケ江倫世, 本同宏成, 奥山正幸, 森春英, 木村淳夫
    • Organizer
      日本応用糖質科学会平成22年度大会
    • Place of Presentation
      静岡県コンベンションアーツセンター
    • Year and Date
      2010-09-15
    • Related Report
      2010 Annual Research Report
  • [Book] 食品の界面制御技術と応用―開発現場と研究最前線を繋ぐ―2011

    • Author(s)
      松村康生・松宮健太郎・小川晃弘
    • Publisher
      シーエムシー出版
    • Related Report
      2011 Final Research Report
  • [Book] 食品の界面制御技術と応用-開発現場と研究最前線を繋ぐ-2011

    • Author(s)
      松村康生・松宮健太郎・小川晃弘
    • Publisher
      シーエムシー出版
    • Related Report
      2011 Annual Research Report

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Published: 2010-08-27   Modified: 2016-04-21  

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