Analysis of p3-Alc, metabolic product of Alcadein

• Project/Area Number: 22890001
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Biological pharmacy
• Research Institution: Hokkaido University
• Principal Investigator: HATA Saori 北海道大学, 大学院・薬学研究院, 助教 (40581012)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥3,146,000 (Direct Cost: ¥2,420,000、Indirect Cost: ¥726,000); Fiscal Year 2011: ¥1,508,000 (Direct Cost: ¥1,160,000、Indirect Cost: ¥348,000); Fiscal Year 2010: ¥1,638,000 (Direct Cost: ¥1,260,000、Indirect Cost: ¥378,000)
• Keywords: アルツハイマー病 / γセクレターゼ / 診断マーカー

Research Abstract

To reveal the pathogenesis of sporadic type of Alzheimer's disease(AD), I analyzed AD-related peptide, p3-Alc, which is the metabolic product of Alcadein protein. In cerebrospinal fluid and blood of subjects with sporadic AD, p3-Alc peptides showed quality and quantity alteration. This result indicates that dysfunction of Alc-cleaving secretase can be acquired and it may link to the onset of sporadic AD.

Research Products

• [Journal Article] Alternative processing of γ-secretase substrates in common forms of mild cognitive impairment and Alzheimer's disease : evidence for γ-secretase dysfunction (2011)
  • Author(s): Hata S
  • Journal Title: Ann.Neurol. Volume: 69 Issue: 6 Pages: 1026-1031
  • DOI: 10.1002/ana.22343
  • Peer Reviewed
• [Journal Article] Coordinated increase ofγ-secretase reaction products in the plasma of some female Japanese sporadic Alzheimer's disease patients : quantitative analysis of p3-Alcαwith a new ELISA system (2011)
  • Author(s): Konno T, Hata S., 他11名
  • Journal Title: Molecular Neurodegeneration Volume: 6 Pages: 76-76
  • Peer Reviewed
• [Journal Article] Coordinated increase of y-secretase products in the plasma of some female Japanese sporadic Alzheimer's disease patients: quantitative analysis of p3-Alca with a new ELISA system (2011)
  • Author(s): Konno, et al.
  • Journal Title: Molecular Neurodegeneration Volume: 6 Issue: 1 Pages: 76-76
  • DOI: 10.1186/1750-1326-6-76
  • Peer Reviewed
• [Journal Article] 新しいバイオマーカー;アルカデイン (2010)
  • Author(s): 羽田沙緒里、今野禎子、鈴木利治
  • Journal Title: Pharma Medica Volume: 28 Pages: 15-19
• [Journal Article] Increased amyloidogenic processing of transgenic human APP in X11-like deficient mouse brain (2010)
  • Author(s): Kondo, M., Shiono, M., Itoh, G., Takei, N., Matsushima, T., Maeda, M., Taru, H., Hata, S., 他3名
  • Journal Title: Molecular Neurodegeneration Volume: 5 Pages: 35-35
  • NAID: 120005158908
  • Peer Reviewed
• [Journal Article] 新しいバイオマーカー;アルカデイン (2010)
  • Author(s): 羽田沙緒里、今野禎子、鈴木利治
  • Journal Title: Pharma Medica. Volume: 28 Pages: 15-19
• [Presentation] アルツハイマー病の新たな生化学的マーカー (2011)
  • Author(s): 羽田沙緒里浦上克哉、谷口美也子、池内健、鈴木利治
  • Organizer: 第1回認知症予防学会学術大会
  • Place of Presentation: 米子コンベンションセンター(鳥取)
• [Presentation] Alternativeγ-secretase processing in sporadic Alzheimer's disease suggested by p3-Alc peptide, metabolic product of Alcadein (2011)
  • Author(s): Hata, S. Urakami, K., Taniguchi, M., Akatsu, H., Gandy, S., Suzuki, T
  • Organizer: Alzheimer's Association International Conference
  • Place of Presentation: Porte de Versailles(France)
• [Presentation] p3-Alcのアルツハイマー病発症サロゲートマーカーおよび診断バイオマーカーとしての応用 (2011)
  • Author(s): 羽田沙緒里
  • Organizer: 日本薬学会第131年会
  • Place of Presentation: ツインメッセ静岡(静岡)
• [Presentation] Alternative γ-secretase processing in sporadic Alzheimer's disease suggested by p3-Alc peptide, metabolic product of Alcadein (2011)
  • Author(s): Hata, S, 他5名
  • Organizer: Alzheimer's Association International Conference 2011
  • Place of Presentation: Porte de Versailles (France)
• [Presentation] Enhanced amyloidogenic processing of APP in Alcadein-deficient mice (2011)
  • Author(s): Gotoh, N., Saito, Y., Hata, S., Yamamoto, T., Suzuki, T
  • Organizer: Alzheimer's Association International Conference 2011
  • Place of Presentation: Porte de Versailles (France)
• [Presentation] Quantification of plasma P3-Alc level with sELISA systems (2011)
  • Author(s): Fang, J., Konno, T., Horikoshi-Sakuraba, Y., Hata, S, 他4名
  • Organizer: Alzheimer's Association International Conference 2011
  • Place of Presentation: Porte de Versailles (France)
• [Presentation] Alcadein epsilon-cleavage followed by gamma-cleavage (2011)
  • Author(s): Piao, Y., Hata, S., Suzuki, T.
  • Organizer: Alzheimer's Association International Conference 2011
  • Place of Presentation: Porte de Versailles (France)
• [Presentation] Altered processing og gamma-secretase substrates and clearance of peptide products (Abeta and p3-Alc) as a pathogenesis of sporadic Alzheimer's disease (2011)
  • Author(s): Suzuki, T., Hata, S
  • Organizer: 9th Asia/Oceania Regional Congress Of Gerontology And Geriatrics 2011
  • Place of Presentation: Melbourne Convention Centre (Australia)(招待講演)
• [Presentation] Enhanced amyloidogenic processing of APP in Alcadeinα-deficient mice (2011)
  • Author(s): Yamamoto, T., Gotoh, N., Saito, Y., Hata, S., Suzuki, T.
  • Organizer: Society for Neuroscience annual meeting, Neuroscience 2011
  • Place of Presentation: Walter E.Washington Convention Center (USA)
• [Presentation] アルツハイマー病の新たな生化学的マーカー (2011)
  • Author(s): 羽田沙緒里, 他4名
  • Organizer: 第1回 認知症予防学会 学術大会
  • Place of Presentation: 米子コンベンションセンター(鳥取)
• [Presentation] Alcadeinαの欠失によりAPPのアミロイド産生的代謝は亢進する (2011)
  • Author(s): 山本融、後藤直也、齊藤有紀、羽田沙緒里、松崎文雄、鈴木利治
  • Organizer: 第34回 日本神経科学大会
  • Place of Presentation: パシフィコ横浜(神奈川)
• [Presentation] Alcadeinのγセクレターゼによる膜内切断機構の解明 (2011)
  • Author(s): 朴懿、羽田沙緒里、鈴木利治
  • Organizer: 第84回日本生化学大会
  • Place of Presentation: 国立京都国際会館(京都)
• [Presentation] アポリポタンパク質EによるAlcadein代謝への影響 (2011)
  • Author(s): 木村彩乃、羽田沙緒里、鈴木利治
  • Organizer: 第84回日本生化学大会
  • Place of Presentation: 国立京都国際会館(京都)
• [Presentation] p3-Alcのアルツハイマー病発症サロゲートマーカーおよび診断バイオマーカーとしての応用 (2011)
  • Author(s): 羽田沙緒里
  • Organizer: 日本薬学会第131年会
  • Place of Presentation: 静岡
• [Presentation] p3-Alc Peptides Are Generated byα-andγ-secretase Cleavage of Alcadein and Indiate Alzheimer's Disease-Relatedγ-secretase Dysfunction (2010)
  • Author(s): Saori Hata
  • Organizer: The American Society for Cell Biology, Annual Meeting
  • Place of Presentation: Pennsylvania Convention Center(USA)
• [Presentation] γ-secretase dysfunction in sporadic Alzheimer's disease suggested by p3-Alc peptide (2010)
  • Author(s): Saori Hata
  • Organizer: metabolic product of Alcadein、Alzheimer's Association ICAD2010
  • Place of Presentation: Hawai' i Convention center(USA)
• [Presentation] Y-secretase dysfunction in sporadic Alzheimer's disease suggested by p3-Alc peptide, metabolic product of Alcadein (2010)
  • Author(s): Hata., S., 他13名
  • Organizer: Alzheimer's Association ICAD2010
  • Place of Presentation: Honolulu USA
• [Presentation] Expression and localization of Alcadein along with X11L and APP in mouse brain (2010)
  • Author(s): Piao,Y., Gotoh, N., Kondo, M., Saito, Y., Hata, S., 他3名
  • Organizer: Alzheimer's Association ICAD2010
  • Place of Presentation: Honolulu USA
• [Presentation] Increased p3-Alca, a Y-secretase reaction product of Alcadein, in plasma of sporadic Alzheimer's disease subjects (2010)
  • Author(s): Konno, T., Hata, S., 他8名
  • Organizer: Alzheimer's Association ICAD2010
  • Place of Presentation: Honolulu USA
• [Presentation] YセクレターゼによるAlcadein代謝産物p3-Alcの血中測定 (2010)
  • Author(s): 今野禎子、羽田沙緒里, 他5名
  • Organizer: 第15回日本病態プロテアーゼ学会学術集会
  • Place of Presentation: 大阪
• [Presentation] YセクレターゼによるAlcadein切断機構 (2010)
  • Author(s): 朴懿、浦野里美、羽田沙緒里、鈴木利治
  • Organizer: 第15回日本病態プロテアーゼ学会学術集会
  • Place of Presentation: 大阪
• [Presentation] AlcadeinのYセクレターゼ代謝産物p3-Alcの血中からの抽出と定量 (2010)
  • Author(s): 今野禎子、羽田沙緒里, 他6名
  • Organizer: 第29回日本認知症学会学術集会
  • Place of Presentation: 名古屋
• [Presentation] YセクレターゼによるAlcadein切断機構 (2010)
  • Author(s): 朴懿、浦野里美、羽田沙緒里、鈴木利治
  • Organizer: 第15回日本病態プロテアーゼ学会学術集会
  • Place of Presentation: 名古屋
• [Presentation] p3-Alc Peptides Are Generated by alpha-and gamma-Secretase Cleavage of Alcadein and Indiate Alzheimer's Disease-Related gamma-secretase Dysfunction (2010)
  • Author(s): Hata.S., Suzuki, T.
  • Organizer: The American Society for Cell Biology, Annual Meeting
  • Place of Presentation: Philadelphia USA
• [Book] Lipids and Cellular Membranes in Amyloid Disease (2011)
  • Author(s): Saori Hata, Yuhki Saito, Toshiharu Suzuki
  • Publisher: Wiley-VCH
• [Book] Lipids and Cellular Membranes in Amyloid Disease (Edited by Jelinek, R.) (2011)
  • Author(s): Hata, S., Saito, Y., Suzuki, T.
  • Publisher: Wiley-VCH
• [Book] Lipids and Cellular Membranes in Amyloid Disease (2011)
  • Author(s): Saori Hata, Yuhki Saito, Toshiharu Suzuki
  • Publisher: Wiley-VCH(in press)
• [Remarks]
  • URL: http://www.pharm.hokudai.ac.jp/shinkei/index.html
• [Remarks]
  • URL: http://www.pharm.hokudai.ac.jp/shinkei/index.html
• [Remarks]
  • URL: http://www.pharm.hokudai.ac.jp/shinkei/index.html
• [Patent(Industrial Property Rights)] アルカデインペプチド切断物による認知症またはアルツハイマー病診断方法または予後予測方法 (2011)
  • Inventor(s): 鈴木利治、伴(羽田)沙緒里、清藤勉、柏原昌次
  • Industrial Property Rights Holder: 国立大学法人北海道大学、株式会社免疫生物研究所
  • Industrial Property Number: 2011-082975
  • Filing Date: 2011-04-04