Effect of oxidative stress on disulfide bond formation of uric acid efflux transporter BCRP

Research Project

Project/Area Number	22890003
Research Category	Grant-in-Aid for Research Activity Start-up
Allocation Type	Single-year Grants
Research Field	Applied pharmacology
Research Institution	Hokkaido University
Principal Investigator	OGURA Jiro 北海道大学, 大学院・薬学研究院, 助教 (20580640)
Project Period (FY)	2010 – 2011
Project Status	Completed (Fiscal Year 2011)
Budget Amount *help	¥3,146,000 (Direct Cost: ¥2,420,000、Indirect Cost: ¥726,000) Fiscal Year 2011: ¥1,508,000 (Direct Cost: ¥1,160,000、Indirect Cost: ¥348,000) Fiscal Year 2010: ¥1,638,000 (Direct Cost: ¥1,260,000、Indirect Cost: ¥378,000)
Keywords	痛風 / キサンチンオキシダーゼ / 活性酸素 / トランスポータ / ジスルフィド結合
Research Abstract	In this study, we investigated that oxidative stress affected BCRP expression, which is a uric acid efflux transporter. It has known that aging-induced oxidative stress is caused by activation of xanthine oxidase. Thus, we used the substrates of xanthine oxidase in this study. In Caco-2 cells, the activation of xanthine oxidase was suppressed the BCRP S-S bond formation, but not in HK-2 cells. These results suggest that the effect of oxidative stress on the efflux of uric acid is different between intestine and kidney.

Report

(3 results)