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Development of highly sensitive methods to analyze signal transduction in a small smooth muscle

Research Project

Project/Area Number 22890011
Research Category

Grant-in-Aid for Research Activity Start-up

Allocation TypeSingle-year Grants
Research Field General physiology
Research InstitutionAsahikawa Medical College

Principal Investigator

TAKEYA Kosuke  旭川医科大学, 医学部, 助教 (20586862)

Project Period (FY) 2010 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥2,964,000 (Direct Cost: ¥2,280,000、Indirect Cost: ¥684,000)
Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2010: ¥1,534,000 (Direct Cost: ¥1,180,000、Indirect Cost: ¥354,000)
Keywords平滑筋 / 毛様体筋 / ミオシン / リン酸化定量法 / キナーゼ / ホスファターゼ
Research Abstract

In order to address the regulatory mechanisms of smooth muscle contraction and relaxation, we aimed to develop a novel highly sensitive method to analyze phosphorylation signals. We are attempting to quantify MYPT1 phosphorylation in bovine ciliary muscle by using phos-tag electrophoresis. High level of myosin phosphorylation was observed in Carbachol-contracted ciliary muscle. Contrary to our expectations, myosin phosphorylation remained at high level even in the resting state. These results suggest that there could be an unknown Ca^<2+>-dependent factor that regulates bovine ciliary muscle contraction rather than myosin phosphorylation.

Report

(3 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • Research Products

    (6 results)

All 2012 Other

All Journal Article (1 results) (of which Peer Reviewed: 1 results) Presentation (2 results) Remarks (3 results)

  • [Journal Article] Phos-tag SDS電気泳動を用いた平滑筋収縮調節タンパク質のリン酸化シグナル解析

    • Author(s)
      竹谷浩介
    • Journal Title

      生物物理化学

      Volume: 56

    • NAID

      130004809448

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Presentation] ウシ毛様体筋に於ける平滑筋ンのリン酸化2012

    • Author(s)
      竹谷浩介
    • Organizer
      日本生理学会
    • Place of Presentation
      松本市総合体育館
    • Year and Date
      2012-03-30
    • Related Report
      2011 Annual Research Report
  • [Presentation] Smooth muscle myosin phosphorylation in bovine ciliary muscle2012

    • Author(s)
      Kosuke Takeya, Motoi Miyazu, Teppei Akao & Akira Takai
    • Organizer
      第89回日本生理学会大会
    • Place of Presentation
      松本
    • Related Report
      2011 Final Research Report
  • [Remarks]

    • URL

      http://www.asahikawa-med.ac.jp/dept/mc/phys1/profiles/takeya.htm

    • Related Report
      2011 Final Research Report
  • [Remarks]

    • URL

      http://www.asahikawa-med.ac.jp/dept/mc/physl/profiles/takeya.htm

    • Related Report
      2011 Annual Research Report
  • [Remarks]

    • URL

      http://www.asahikawa-med.ac.jp/dept/mc/physl/profiles/takeya.htm

    • Related Report
      2010 Annual Research Report

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Published: 2010-08-27   Modified: 2016-04-21  

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