| Budget Amount *help |
¥2,964,000 (Direct Cost: ¥2,280,000、Indirect Cost: ¥684,000)
Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2010: ¥1,534,000 (Direct Cost: ¥1,180,000、Indirect Cost: ¥354,000)
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| Research Abstract |
In order to address the regulatory mechanisms of smooth muscle contraction and relaxation, we aimed to develop a novel highly sensitive method to analyze phosphorylation signals. We are attempting to quantify MYPT1 phosphorylation in bovine ciliary muscle by using phos-tag electrophoresis. High level of myosin phosphorylation was observed in Carbachol-contracted ciliary muscle. Contrary to our expectations, myosin phosphorylation remained at high level even in the resting state. These results suggest that there could be an unknown Ca^<2+>-dependent factor that regulates bovine ciliary muscle contraction rather than myosin phosphorylation.
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