Identification of regulatory B cells in a murine model of primary biliary cirrhosis and fundamental examination for clinical application

• Project/Area Number: 22890024
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Gastroenterology
• Research Institution: Akita University
• Principal Investigator: MORITOKI Yuki 秋田大学, 医学部, 講師 (90585522)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥3,068,000 (Direct Cost: ¥2,360,000、Indirect Cost: ¥708,000); Fiscal Year 2011: ¥1,469,000 (Direct Cost: ¥1,130,000、Indirect Cost: ¥339,000); Fiscal Year 2010: ¥1,599,000 (Direct Cost: ¥1,230,000、Indirect Cost: ¥369,000)
• Keywords: 原発性胆汁性肝硬変 / B細胞 / CD20 / CD5

Research Abstract

Location of CD5 expressing B cells, supposed to have a regulatory function in an inflammatory condition, was examined using immunohistochemical double staining for CD20 and CD5 in the liver samples from the patients of primary biliary cirrhosis and chronic hepatitis C. Both liver samples demonstrated CD20 and CD5 double-positive B cells in follicle-like aggregations in the portal areas, while B cells were CD5 negative in ductal invasion, suggesting unlikely contribution of CD20 and CD5 double-positive B cells directly to biliary epithelial damage in the livers of primary biliary cirrhosis.

Research Products

• [Journal Article] Comparative analysis of portal cell infiltrates in antimitochondrial autoantibody-positive versus antimitochondrial autoantibody-negative primary biliary cirrhosis (2012)
  • Author(s): Jin Q, Moritoki Y, Lleo A, Tsuneyama K, Invernizzi P, Moritoki H, Kikuchi K, Lian ZX, Hirschfield GM, Ansari AA, Coppel RL, Gershwin ME, Niu J
  • Journal Title: Hepatology Volume: 55 Issue: 5 Pages: 1495-506
  • DOI: 10.1002/hep.25511
  • Peer Reviewed
• [Journal Article] Biochemical and immunologic effects of rituximab in patients with primary biliary cirrhosis and an incomplete response to ursodeoxycholic acid (2012)
  • Author(s): Tsuda M, Moritoki Y, Lian ZX, Zhang W, Yoshida K, Wakabayashi K, Yang GX, Nakatani T, Vierling J, Lindor K, Gershwin ME, Bowlus CL
  • Journal Title: Hepatology Volume: 55 Issue: 2 Pages: 512-21
  • DOI: 10.1002/hep.24748
  • Peer Reviewed
• [Journal Article] Pathological Features of New Animal Models for Primary Biliary Cirrhosis (2011)
  • Author(s): Tsuneyama K, Moritoki Y, Kikuchi K, Nakanuma Y
  • Journal Title: International Journal of Hepatology Volume: 2012 Pages: 1-7
  • NAID: 120004186279
  • URL: http://www.hindawi.com/journals/ijhep/2012/403954/
  • Peer Reviewed
• [Journal Article] B cells promote hepatic inflammation, biliary cyst formation, and salivary gland inflammation in the NOD. c3c4 model of autoimmune cholangitis (2011)
  • Author(s): Moritoki Y, Tsuda M, Tsuneyama K, Zhang W, Yoshida K, Lian ZX, Yang GX, Ridgway WM, Wicker LS, Ansari AA, Gershwin ME
  • Journal Title: Cell Immunol Volume: 268 Pages: 16-23
  • URL: http://www.sciencedirect.com/science/article/pii/S0008874911000153
  • Peer Reviewed
• [Journal Article] B cells promote hepatic inflammation, biliary cyst formation, and salivary gland inflammation in the NOD.c3c4 model of autoimmune cholangitis (2011)
  • Author(s): Yuki Moritoki, et al
  • Journal Title: Cell Immunol Volume: VOL.268 Pages: 16-23
  • Peer Reviewed
• [Journal Article] Murine models of autoimmune cholangitis (2010)
  • Author(s): Ueno Y, Ambrosini YM, Moritoki Y, Ridgway WM, Gershwin ME
  • Journal Title: Curr Opin Gastroenterol Volume: 26 Issue: 3 Pages: 274-9
  • DOI: 10.1097/mog.0b013e32833755aa
• [Journal Article] Murine models of autoimmune cholangitis (2010)
  • Author(s): Ueno Y, Ambrosini YM, Moritoki Y, et al
  • Journal Title: Curr Opin Gastroenterol Volume: 26 Pages: 274-279
• [Presentation] 自己免疫性胆管炎モデルNOD.c3c4マウスにおける胆管炎および唾液腺炎に対するB細胞の炎症促進的役割 (2011)
  • Author(s): 守時由起, 他
  • Organizer: 第15回日本肝臓学会大会
  • Place of Presentation: 福岡国際会議場(福岡県)
  • Year and Date: 2011-10-20
• [Presentation] A critical role of B cells in biliary disease and sialadenitis in the NOD.c3c4 model of autoimmune cholangitis (2011)
  • Author(s): Yuki Moritoki, et al.
  • Organizer: Immunology 2011, 98^<th> Annual Meeting The American Association of Immunologists
  • Place of Presentation: Moscone Center, (San Francisco, CA, USA)
  • Year and Date: 2011-05-14
• [Presentation] A critical role of B cells in biliary disease and sialadenitis in the NOD. c3c4 model of c3c4 model ofautoimmune chautoimmune cholangitis (2011)
  • Author(s): Yuki Moritoki, Masanobu Tsuda, Koichi Tsuneyama, Weici Zhang, Katsunori Yoshida, Zhe-Xiong Lian, Guo-Xiang Yang, William M. Ridgway Linda S., Wicker, Aftab, A. Ansari, M. Eric Gershwin
  • Organizer: Immunology 2011
  • Place of Presentation: San Francisco, US
• [Presentation] CD45ROhighCD57+CD8high T cells in primary biliary cirrhosis (2011)
  • Author(s): Masanobu Tsuda, Yoko Ambrosini, Yuki Moritoki, Katsunori Yoshida, Weici Zhang, Guo-Xiang Yang, Yugo Ando, Guanghua Rong, Toshio Nakatani, Christopher Bowlus, Aftab Ansari, Zhe-Xiong Lian, M. Eric Gershwin
  • Organizer: Immunology 2011
  • Place of Presentation: San Francisco, US
• [Presentation] 自己免疫性胆管炎モデルNOD. c3c4マウスにおける胆管炎および唾液腺炎に対するB細胞の炎症促進的役割 (2011)
  • Author(s): 守時由起, 常山幸一, M. E. Gershwin
  • Organizer: 第15回日本肝臓学会大会(第19回日本消化器病関連学会週間)
  • Place of Presentation: 福岡
• [Presentation] PBC患者体内におけるIgM産生の場についての検討 (2011)
  • Author(s): 菊池健太郎, 常山幸一, 守時由起, 細谷直美, 梶山祐介, 高井敦子, 根岸雅嗣, 原眞純, 水口國雄, 宮川浩
  • Organizer: 第47回日本肝臓学会総会
  • Place of Presentation: 東京
• [Presentation] 原発性胆汁性肝硬変におけるTGF-βシグナル伝達機構の解析 (2011)
  • Author(s): 川村梨那子, 松崎恒一, 吉田勝紀, 常山幸一, 守時由起, 井口亮輔, 村田美樹, 山口隆志, 仲野俊成, 廣原淳子, 関寿人, 岡崎和一
  • Organizer: 第47回日本肝臓学会総会
  • Place of Presentation: 東京
• [Presentation] 核酸アナログ治療後、B型慢性肝疾患の線維化・癌化シグナルは回帰する (2011)
  • Author(s): 吉田勝紀, Long Qing, 松崎恒一, 常山幸一, 守時由起, Zhe-Xiong Lian, Junqi Niu, 井口亮輔, 村田美樹, 川村梨那子, 山口隆志, 関寿人, 岡崎和一
  • Organizer: 第47回日本肝臓学会総会
  • Place of Presentation: 東京
• [Presentation] Disease course dependent opposite roles of B cells to pathogenesis and pathophysiology of autoimmune cholangitis in dnTGF-ssRII mice (2010)
  • Author(s): Y.Moritoki, et al
  • Organizer: 14^<th> International Congress of Immunology
  • Place of Presentation: Kobe International Exhibition Hall (Hyogo, Japan)
  • Year and Date: 2010-08-26
• [Presentation] 抗CD20抗体療法による自己免疫性胆管炎の改善および腸炎の増悪 (2010)
  • Author(s): 守時由起, 他
  • Organizer: 第46回日本肝臓学会総会
  • Place of Presentation: 山形国際ホテル(山形県)
  • Year and Date: 2010-05-28
• [Presentation] Disease course dependent opposite roles of B cells to pathogenesis and pathophysiology of autoimmune cholangitis in dnTGF-βRII mice (2010)
  • Author(s): Y. Moritoki, Z. Lian, K. Tsuneyama, W. Zhang, K. Yoshida, K. Wakabayashi, G. Yang, Y. Ueno, M. Gershwin
  • Organizer: 14^<th>International Congress of Immunology
  • Place of Presentation: Kobe Japan
• [Presentation] 抗CD20抗体療法による自己免疫性胆管炎の改善および腸炎の増悪 (2010)
  • Author(s): 守時由起, Zhe-Xiong LIAN, 常山幸一, Weici Zhang, 菊池健太郎, 宮川浩, 上野義之, M. Eric GERSHWIN
  • Organizer: 第46回日本肝臓学会総会
  • Place of Presentation: 山形
• [Presentation] C型慢性肝炎患者における血清B cell activating factor (BAFF)濃度と橋本病の関連および血清Estrogenの影響 (2010)
  • Author(s): 梶山祐介, 河井敏宏, 高井敦子, 本田宗宏, 細谷直美, 守時由起, 宮川浩, 菊池健太郎
  • Organizer: 第46回日本肝臓学会総会
  • Place of Presentation: 山形
• [Presentation] Monosodium Glutamate投与NASHモデルマウスにおけるメタボリックシンドロームの検討 (2010)
  • Author(s): 高井敦子, 梶山祐介, 河井敏宏, 根岸雅嗣, 藤本誠, 細谷直美, 蔵野信, 守時由起, 常山幸一, 原眞純, 塚本和久, 宮川浩, 菊池健太郎
  • Organizer: 第46回日本肝臓学会総会
  • Place of Presentation: 山形
• [Remarks]
  • URL: http://www.crc.akita-u.ac.jp/researchers/researcher_detail.php?idx=820