The functional analysis by structural change of Chromogranin in the neuropsychiatric diseases

• Project/Area Number: 22890026
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Psychiatric science
• Research Institution: University of Tsukuba
• Principal Investigator: IIJIMA Yoshimi 筑波大学, 医学医療系, 助教 (70574648)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥3,068,000 (Direct Cost: ¥2,360,000、Indirect Cost: ¥708,000); Fiscal Year 2011: ¥1,469,000 (Direct Cost: ¥1,130,000、Indirect Cost: ¥339,000); Fiscal Year 2010: ¥1,599,000 (Direct Cost: ¥1,230,000、Indirect Cost: ¥369,000)
• Keywords: クロモグラニン / 関連解析 / SNP / 統合失調症 / 筋萎縮性側索硬化症 / SOD1 / KOマウス

Research Abstract

In our previous genome-wide association studies, a significant association with schizophrenia was observed at the chromogranin B (CHGB) gene locus. CHGB is a widespread family of proteins in the neurosystem, whose function is guiding the sorting and secretion of neuropeptides. In this study, I perform to functional analysis of CHGB using human sample and genetically modified animal. I confirmed that the gene variation of CHGB was significantly related to schizophrenia using a large-scale sample. Furthermore, I found that the plasma CHGB protein level is high in a schizophrenia patient, and the plasma CHGB protein level related to the severity of the stress reaction.

Research Products

• [Journal Article] Phencyclidine-Induced Decrease of Synaptic Connectivity via Inhibition of BDNF Secretion in Cultured Cortical Neurons (2012)
  • Author(s): Adachi N, Numakawa T, Kumamaru E, Itami C, Chiba S, Iijima Y, Richards M, Katoh-Semba R, Kunugi H.
  • Journal Title: Cereb Cortex
  • URL: http://www.ncbi.nlm.nih.gov/pubmed/22467667
  • Peer Reviewed
• [Journal Article] A two-stage case-control association study between the tryptophan hydroxylase2 (TPH2) gene and schizophrenia in a Japanese population (2012)
  • Author(s): Watanabe Y, Egawa J, Iijima Y, Nunokawa A, Kaneko N, Shibuya M, Arinami T, Ujike H, Inada T, Iwata N, Tochigi M, Kunugi H, Itokawa M, Ozaki N, Hashimoto R, Someya T
  • Journal Title: Schizophr Res
  • URL: http://www.ncbi.nlm.nih.gov/pubmed/22342331
  • Peer Reviewed
• [Journal Article] Further evidence for a male-selective genetic association of synapse-associated protein 97 (SAP97) gene with schizophrenia (2012)
  • Author(s): Uezato A, Kimura-Sato J, Yamamoto N, Iijima Y, Kunugi H, Nishikawa T
  • Journal Title: Behav Brain Funct Volume: 8(1) Pages: 2-2
  • URL: http://www.ncbi.nlm.nih.gov/pubmed/22225629
  • Peer Reviewed
• [Journal Article] Association of SNPs linked to increased expression of SLC1A1 with schizophrenia (2012)
  • Author(s): Horiuchi Y, Iida S, Koga M, Ishiguro H, Iijima Y, Inada T, Watanabe Y, Someya T, Ujike H, Iwata N, Ozaki N, Kunugi H, Tochigi M, Itokawa M, Arai M, Niizato K, Iritani S, Kakita A, Takahashi H, Nawa H, Arinami T
  • Journal Title: Am J Med Genet B Neuropsychiatr Genet Volume: 159B(1) Issue: 1 Pages: 30-37
  • DOI: 10.1002/ajmg.b.31249
  • Peer Reviewed
• [Journal Article] Possible association between Interleukin-1beta gene and schizophrenia in a Japanese population (2012)
  • Author(s): Sasayama D, Hori H, Teraishi T, Hattori K, Ota M, Iijima Y, Tatsumi M, Higuchi T, Amano N, Kunugi H
  • Journal Title: Behav Brain Funct Volume: 7(1) Pages: 35-35
  • NAID: 120007110616
  • URL: http://www.ncbi.nlm.nih.gov/pubmed/21843369
  • Peer Reviewed
• [Journal Article] Modulation of cortisol responses to the DEX/CRH test by polymorphisms of the interleukin-1beta gene in healthy adults (2012)
  • Author(s): Sasayama D, Hori H, Iijima Y, TeraishiT, Hattori K, Ota M, Fujii T, Higuchi T, Amano N, Kunugi H
  • Journal Title: Behav Brain Funct Volume: 7(1) Pages: 23-23
  • NAID: 120007110615
  • URL: http://www.ncbi.nlm.nih.gov/pubmed/21726461
  • Peer Reviewed
• [Journal Article] A two-stage case-control association study between the tryptophan hydroxylase 2 (TPH2) gene and schizophrenia in a Japanese population (2012)
  • Author(s): Watanabe Y, Egawa J, Iijima Y, Nunokawa A, Kaneko N, Shibuya M, Arinami T, Ujike H, Inada T, Iwata N, Tochigi M, Kunugi H, Itokawa M, Ozaki N, Hashimoto R, Someya T
  • Journal Title: Schizophr Res Volume: Feb17(Epub ahead of print)
  • Peer Reviewed
• [Journal Article] Phencyclidine-Induced Decrease of Synaptic Connectivity via Inhibition of BDNF Secretion in Cultured Cortical Neurons (2012)
  • Author(s): Adachi N, Numakawa T, Kumamaru E, Itami C, Chiba S, Iijima Y, Richards M, Katoh-Semba R, Kunugi H
  • Journal Title: Cereb Cortex Volume: Mar30(Epub ahead of print)
  • Peer Reviewed
• [Journal Article] Possible association between Interleukin-1beta gene and schizophrenia in a Japanese population (2011)
  • Author(s): Sasayama D, Hori H, Iijima Y, Teraishi T, Hattori K, Ota M, Fujii T, Higuchi T, Amano N, Kunugi H
  • Journal Title: Behav Brain Funct Volume: 6;7(1)(Epub ahead of print) Pages: 35-35
  • NAID: 120007110616
  • Peer Reviewed
• [Journal Article] Further evidence for a male-selective genetic association of synapse-associated protein 97 (SAP97) gene with schizophrenia (2011)
  • Author(s): Uezato A, Kimura-Sato J, Yamamoto N, Iijima Y, Kunugi H, Nishikawa T
  • Journal Title: Behav Brain Funct Volume: 8(1)(Epub ahead of print) Pages: 2-2
  • Peer Reviewed
• [Presentation] SLC1A1遺伝子多型と統合失調症との関連解析 (2011)
  • Author(s): 飯田周平,堀内泰江,飯嶋良味,石黒浩毅,稲田俊也,渡部雄一郎,染谷俊幸,氏家寛,岩田仲生,尾崎紀夫,功刀浩,栃木衛,糸川昌成,新井誠,新里和弘,入谷修司,柿田明美,高崎均,那波宏之,有波忠雄
  • Organizer: 第56回日本人類遺伝学会・第11回東アジア人類遺伝学会共同大会
  • Place of Presentation: 幕張メッ
  • Year and Date: 2011-11-12
• [Presentation] フェンサイクリジンが引き起こすシナプス減少にBDNF の機能障害が関与する:Possible involvement of BDNF dysfunction in the synaptic loss caused by phencyclidine (2011)
  • Author(s): 安達直樹,沼川忠広,熊丸絵美,伊丹千晶,千葉秀一,飯嶋良味,Misty Richards,仙波りつ子,功刀浩
  • Organizer: 第34回日本神経科学学会
  • Place of Presentation: パシフ Bコ横浜
  • Year and Date: 2011-09-17
• [Presentation] Possible involvement of brain-derived neurotrophic factor (BDNF) dysfunction in synaptic loss caused by phencyclidine (2011)
  • Author(s): Adachi N, Numakawa T, Kumamaru E, ItamiC, Iijima Y, Richards M, Katoh-Semba R andKunugi H
  • Organizer: BIT Life Sciences' 4th Annual Protein and Peptide Conference
  • Place of Presentation: Beijing, China
  • Year and Date: 2011-03-23
• [Presentation] フェンサイクリジンが引き起こすシナプス減少にBDNFの機能障害が関与する:Possible involvement of BDNF dysfunction in the synaptic loss caused by phencyclidine (2011)
  • Author(s): 安達直樹, 沼川忠広, 熊丸絵美, 伊丹千晶, 千葉秀一, 飯嶋良味, Misty Richards, 仙波りつ子, 功刀浩
  • Organizer: 第34回日本神経科学学会
  • Place of Presentation: パシフィコ横浜(神奈川県)
• [Presentation] SLC1A1遺伝子多型と統合失調症との関連解析 (2011)
  • Author(s): 飯田周平, 堀内泰江, 飯嶋良味, 石黒浩毅, 稲田俊也, 渡部雄一郎, 染谷俊幸, 氏家寛, 岩田仲生, 尾崎紀夫, 功刀浩, 栃木衛, 糸川昌成, 新井誠, 新里和弘, 入谷修司, 柿田明美, 高崎均, 那波宏之, 有波忠雄
  • Organizer: 第56回日本人類遺伝学会・第11回東アジア人類遺伝学会共同大会
  • Place of Presentation: 幕張メッセ(千葉県)
• [Presentation] ChromograninB遺伝子プロモーター領域の多型と統合失調症との性特異的関連 (2010)
  • Author(s): 飯嶋良味,内山博文,山本宣子,荒木俊弥,堀弘明,巽雅彦,有馬邦正,有波忠雄,功刀浩
  • Organizer: 第55回日本人類遺伝学会
  • Place of Presentation: 大宮ソニックシティ
  • Year and Date: 2010-10-28
• [Presentation] ChromograninB遺伝子プロモーター領域の多型と統合失調症との性特異的関連 (2010)
  • Author(s): 飯嶋良味
  • Organizer: 第55回日本人類遺伝学会
  • Place of Presentation: 大宮ソニックシティ(埼玉県)
  • Year and Date: 2010-10-28
• [Presentation] PHENCYCLIDINE DECREASES EXCITATORY SYNAPSES : POSSIBLE INVOLVEMENT OF DYSFUNCTION OF BRAIN-DERIVED NEUROTROPHIC FACTOR (2010)
  • Author(s): Adachi N, Numakawa T, Kumamaru E, Itami C, Iijima Y, Richards M, Katoh-Semba R, and Kunugi H
  • Organizer: The Asian-Pacific Society for Neurochemistry (APSN)
  • Place of Presentation: Phuket Island, Thailand
  • Year and Date: 2010-10-17
• [Presentation] ChromograninBの発現調節に影響する多型と統合失調症との関連解析 (2010)
  • Author(s): 飯嶋良味,内山博文,山本宣子,堀弘明,巽雅彦,有馬邦正,有波忠雄,功刀浩
  • Organizer: 第18回日本精神行動遺伝学会
  • Place of Presentation: 東北大学医学部5号館201号総合研究棟会議室
  • Year and Date: 2010-09-18
• [Presentation] ChromograninBの発現調節に影響する多型と統合失調症との関連解析 (2010)
  • Author(s): 飯嶋良味
  • Organizer: 第18回日本精神行動遺伝学会
  • Place of Presentation: 東北大学医学部5号館201号(宮城県)
  • Year and Date: 2010-09-18
• [Remarks] 総説飯嶋良味,有波忠雄:統合失調症.最近のGWAS研究の成果.Bio Clinica,Vol.25,No.6,34-39,2010.