Profiling and functional analysis of viral microRNA in EBV-associated gastric carcinoma

• Project/Area Number: 22890037
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Human pathology
• Research Institution: The University of Tokyo
• Principal Investigator: SHINOZAKI Aya 東京大学, 大学院・医学系研究科, 助教 (60581824)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥3,146,000 (Direct Cost: ¥2,420,000、Indirect Cost: ¥726,000); Fiscal Year 2011: ¥1,508,000 (Direct Cost: ¥1,160,000、Indirect Cost: ¥348,000); Fiscal Year 2010: ¥1,638,000 (Direct Cost: ¥1,260,000、Indirect Cost: ¥378,000)
• Keywords: EBV / マイクロRNA / 胃癌 / アポトーシス

Research Abstract

The expression profile of viral microRNAs in EBV-associated gastric carcinoma was analysed using quantitative RT-PCR analysis. Compared with the previous reports on the expression profile of other EBV-associated tumors, some viral microRNAs were specifically upregulated in EBV-associated gastric carcinoma. In vitro analysis revealed that one of these micro RNAs targets a gene that is known to facilitate apoptosis of the cells, and that it subsequently leads to the immortality of tumor cells.

Research Products

• [Journal Article] SOX9 expression and its methylation status in gastric cancer (2012)
  • Author(s): Sun M, Uozaki H, Hino R, Kunita A, Shinozaki A, Ushiku T, Hibiya T,Takeshita K, Isogai M, Takada K,Fukayama M
  • Journal Title: Virchows Archiv Volume: 460 Issue: 3 Pages: 271
  • DOI: 10.1007/s00428-012-1201-7
  • Peer Reviewed
• [Journal Article] Transcriptional factor typing with SOX2, HNF4aP1, and CDX2 closely relates to tumor invasion and Epstein-Barr virus status in gastric cancer (2011)
  • Author(s): Uozaki H, Barua RR, Minhua S, Ushiku T, Hino R, Shinozaki A, Sakatani T, Fukayama M.
  • Journal Title: Int J Clin Exp Pathol Volume: 4(3) Pages: 230-240
  • URL: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3071656/?tool=pubmed
  • Peer Reviewed
• [Journal Article] Expression of ribonucleotide reductase M2subunit in gastric cancer and effects of RRM2 inhibition in vitro (2010)
  • Author(s): Morikawa T, Hino R, Uozaki H, Maeda D, Ushiku T, Shinozaki A,Sakatani T, Fukayama M
  • Journal Title: Human Pathology Volume: Vol.41 Issue: 12 Pages: 1742-1748
  • DOI: 10.1016/j.humpath.2010.06.001
  • Peer Reviewed
• [Presentation] 胃癌におけるProgrammed cell death 4発現とmicroRNA-21発現の検討 (2012)
  • Author(s): 日比谷孝志、坂谷貴司、篠崎綾、孫敏華、日野るみ、牛久哲男、宇於崎宏、磯貝まや、深山正久
  • Organizer: 第69回日本癌学会学術総会
  • Place of Presentation: 大阪国際会議場(大阪府)
• [Presentation] EBウイルス関連胃癌におけるmicroRNA-141の発現と、発現制御機構としてのメチル化の関係 (2011)
  • Author(s): 日比谷孝志、坂谷貴司、篠崎綾、孫敏華、日野るみ、牛久哲男、磯貝まや、石川俊平、宇於崎宏、深山正久
  • Organizer: 第8回EBウイルス研究会
  • Place of Presentation: 大阪大学(大阪府)
  • Year and Date: 2011-07-08
• [Presentation] MicroRNAの病理学 (2011)
  • Author(s): 篠崎綾
  • Organizer: 第100回日本病理学会総会
  • Place of Presentation: パシフィコ横浜(神奈川県)
  • Year and Date: 2011-04-28
• [Presentation] MicroRNAの病理学 (2011)
  • Author(s): 篠崎綾
  • Organizer: 日本病理学会
  • Place of Presentation: パシフィコ横浜(神奈川県)(招待講演)
  • Year and Date: 2011-04-28
• [Presentation] 胃癌におけるSOX2とSOX9蛋白質の消失進行ステージとEBV感染との関連 (2011)
  • Author(s): 孫敏華、宇於崎宏、日野るみ、篠崎綾、日比谷孝志、牛久哲男、深山正久
  • Organizer: 第100回日本病理学会総会
  • Place of Presentation: パシフィコ横浜(神奈川県)
• [Presentation] EBウイルス関連胃癌におけるmicroRNA-21とProgrammed cell death 4発現の検討 (2011)
  • Author(s): 日比谷孝志、坂谷貴司、篠崎綾、孫敏華、日野るみ、牛久哲男、磯貝まや、石川俊平、宇於崎宏、深山正久
  • Organizer: 第100回日本病理学会総会
  • Place of Presentation: パシフィコ横浜(神奈川県)
• [Presentation] 胃癌におけるSOX9の発現低下は進行度及びEBV感染と相関する (2011)
  • Author(s): 孫敏華、宇於崎宏、日野るみ、篠崎綾,牛久哲男,深山正久
  • Organizer: 第69回日本癌学会学術総会
  • Place of Presentation: 大阪国際会議場(大阪府)