The charactarization of herpesvirus protein kinase.
Project/Area Number |
22890039
|
Research Category |
Grant-in-Aid for Research Activity Start-up
|
Allocation Type | Single-year Grants |
Research Field |
Virology
|
Research Institution | The University of Tokyo |
Principal Investigator |
KATO Akihisa 東京大学, 医科学研究所, 助教 (40581187)
|
Project Period (FY) |
2010 – 2011
|
Project Status |
Completed (Fiscal Year 2011)
|
Budget Amount *help |
¥3,146,000 (Direct Cost: ¥2,420,000、Indirect Cost: ¥726,000)
Fiscal Year 2011: ¥1,508,000 (Direct Cost: ¥1,160,000、Indirect Cost: ¥348,000)
Fiscal Year 2010: ¥1,638,000 (Direct Cost: ¥1,260,000、Indirect Cost: ¥378,000)
|
Keywords | 単純ヘルペスウイルス / リン酸化 / 病態発現 / vdUTPase / HSV / Us3 / VP13/14 / ヘルペス性角膜炎 |
Research Abstract |
Herpes simplex virus-1 (HSV-1) encodes at least two protein kinases, Us3 and UL13. In this study, Us3 in vitro kinase assay system shown that Us3 phosphorylates viral protein UL47 at Ser-77 and dUTPase at Ser-187. Interestingly, replacement of UL47 Ser-77 by alanine reduced viral replication in the mouse cornea and the development of herpes stromal keratitis in mice. In contrast, replacement of dUTPase Ser-187 by alanine reduced the HSV-1 neurovirulence.
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Report
(3 results)
Research Products
(14 results)