The charactarization of herpesvirus protein kinase.

• Project/Area Number: 22890039
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Virology
• Research Institution: The University of Tokyo
• Principal Investigator: KATO Akihisa 東京大学, 医科学研究所, 助教 (40581187)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥3,146,000 (Direct Cost: ¥2,420,000、Indirect Cost: ¥726,000); Fiscal Year 2011: ¥1,508,000 (Direct Cost: ¥1,160,000、Indirect Cost: ¥348,000); Fiscal Year 2010: ¥1,638,000 (Direct Cost: ¥1,260,000、Indirect Cost: ¥378,000)
• Keywords: 単純ヘルペスウイルス / リン酸化 / 病態発現 / vdUTPase / HSV / Us3 / VP13/14 / ヘルペス性角膜炎

Research Abstract

Herpes simplex virus-1 (HSV-1) encodes at least two protein kinases, Us3 and UL13. In this study, Us3 in vitro kinase assay system shown that Us3 phosphorylates viral protein UL47 at Ser-77 and dUTPase at Ser-187. Interestingly, replacement of UL47 Ser-77 by alanine reduced viral replication in the mouse cornea and the development of herpes stromal keratitis in mice. In contrast, replacement of dUTPase Ser-187 by alanine reduced the HSV-1 neurovirulence.

Research Products

• [Journal Article] Herpes simplex virus 1 VP22 regulates translocation of multiple viral and cellular proteins and promotes (2012)
  • Author(s): Tanaka M, Kato A, Satoh Y, Ide T, Sagou K, Kimura K, Hasegawa H, Kawaguchi Y.
  • Journal Title: neurovirulence Volume: 86(9) Issue: 9 Pages: 5264-77
  • DOI: 10.1128/jvi.06913-11
  • Peer Reviewed
• [Journal Article] Herpes simplex virus 1 protein kinase Us3 and major tegument protein UL47 reciprocally regulate their subcellular localization in infected cells (2011)
  • Author(s): Kato A, Liu Z, Minowa A, et al
  • Journal Title: Journal of Virology Volume: 85 Issue: 18 Pages: 9599-9613
  • DOI: 10.1128/jvi.00845-11
  • Peer Reviewed
• [Journal Article] Role of the herpes simplex virus 1 Us3 kinase phosphorylation site and endocytosis motifs in the intracellular transport and neurovirulence of envelope glycoprotein B (2011)
  • Author(s): 今井孝彦, 有井潤, 箕輪敦子, 垣本彩, 小柳直人, 加藤哲久, 川口寧
  • Journal Title: Journal of Virology Volume: Vol85 Issue: 10 Pages: 5003-5015
  • DOI: 10.1128/jvi.02314-10
  • Peer Reviewed
• [Presentation] 単純ヘルペスウイルス 1 型プロテインキナーゼUs3による神経病性発現の分子機構 (2012)
  • Author(s): 加藤哲久
  • Organizer: 第60回日本ウイルス学会学術集会
  • Place of Presentation: グランキューブ大阪(大阪国際会議場)
• [Presentation] The catalytic activity of HSV-1 dUTPase and its regulation are specifically required for efficient viral replication and pathogenicity in the brain. (2012)
  • Author(s): Akihisa Kato
  • Organizer: 37th Annual International Herpesvirus Workshop(IHW)
  • Place of Presentation: Calgary-Alberta, Canada
• [Presentation] HSV PK Us3が司る病態発現機構 (2012)
  • Author(s): 加藤哲久
  • Organizer: 第27回ヘルペスウイルス研究会
  • Place of Presentation: 愛知健康プラザ健康宿泊プラザホール
• [Presentation] HSV PK Us3が司る病態発現機構 (2012)
  • Author(s): 加藤哲久
  • Organizer: 第27回ヘルペスウイルス研究会
  • Place of Presentation: あいち健康プラザ健康宿泊舘プラザホール
• [Presentation] HSV-1 Us3によるMHC Class I阻害の分子機構と生体レベルにおける意義 (2012)
  • Author(s): 加藤哲久
  • Organizer: 第27回ヘルペスウイルス研究会
  • Place of Presentation: あいち健康プラザ健康宿泊舘プラザホール
• [Presentation] The catalytic activity of HSV-1 dUTPase and its regulation are specifically required for efficient viral replication and pathogenicity in the brain (2012)
  • Author(s): Akihisa Kato
  • Organizer: 37th Annual International Herpersvirus Workshop (IHW)
  • Place of Presentation: Calgary- Alberta, Canada
• [Presentation] 単純ヘルペスウイルス１型プロテインキナーゼUs3による神経病原性発現の分子機構 (2012)
  • Author(s): 加藤哲久
  • Organizer: 第60回日本ウイルス学会学術集会
  • Place of Presentation: グランキューブ大阪（大阪国際会議場）
• [Presentation] 単純ヘルペスウイルス１型プロテインキナーゼUs3による宿主免疫回避機構と生体レベルにおける意義 (2012)
  • Author(s): 加藤哲久
  • Organizer: 第60回日本ウイルス学会学術集会
  • Place of Presentation: グランキューブ大阪（大阪国際会議場）
• [Presentation] リン酸化プロテオミクスによるHSV感染細胞における蛋白質リン酸化状態の包括的解析とそれに基づく神経 特異的病態発現機構の解明 (2011)
  • Author(s): 加藤哲久
  • Organizer: 第26回ヘルペスウイルス研究会
  • Place of Presentation: 大阪アカデミアグランドホール
  • Year and Date: 2011-06-24
• [Presentation] Phosphoproteomic analysis reveals an HSV-1 kinase-mediated phosphorylation event involved specifically in the regulation of viral neurovirulence. (2011)
  • Author(s): Akihisa Kato
  • Organizer: 36th Annual International Herpesvirus Workshop(IHW)
  • Place of Presentation: Gdansk, Poland.
• [Book] 日本臨牀 (2012)
  • Author(s): 加藤哲久
  • Publisher: 日本臨牀社