Analysis of antibody-dependent cellular virus inhibition(ADCVI) against SIV-infected cells

• Project/Area Number: 22890040
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Virology
• Research Institution: National Institute of Infectious Diseases (2011); The University of Tokyo (2010)
• Principal Investigator: YAMAMOTO Hiroyuki 国立感染症研究所, エイズ研究センター, 研究員 (80574615)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥3,146,000 (Direct Cost: ¥2,420,000、Indirect Cost: ¥726,000); Fiscal Year 2011: ¥1,508,000 (Direct Cost: ¥1,160,000、Indirect Cost: ¥348,000); Fiscal Year 2010: ¥1,638,000 (Direct Cost: ¥1,260,000、Indirect Cost: ¥378,000)
• Keywords: ウイルス / 感染症 / 動物 / ゲノム / 遺伝子

Research Abstract

AIDS virus neutralizing antibodies can play a critical role in acute phase non-sterile virus replication control in vivo. To assess the contribution of antibody-mediated innate inhibitory effects on virus-infected cells within this process, an in vitro antibody-dependent cellular virus inhibition(ADCVI) assay system was established. Virus-binding avidity of antibodies correlated with ADCVI while they did not correlate with neutralizing activity. For direct estimation of ADCVI contribution, anti-SIV antibodies selectively withholding ADCVI but not neutralizing activity were passively immunized against SIV-infected macaques, which did not result in palpable viremia reduction or exertion of selective pressure in viral genome sequences. Taken together, the role of ADCVI in neutralizing antibody-based non-sterile SIV control in vivo was suggested to be limited.

Research Products

• [Journal Article] Impact of vaccination on cytotoxic T lymphocyte immunodominance and cooperation against simian immunodeficiency virus replication in rhesus macaques (2012)
  • Author(s): Ishii H, Kawada M, Tsukamoto T, Yamamoto H, Matsuoka S, Shiino T, Takeda A, Inoue M, Iida A, Hara H, Shu T, Hasegawa M, Naruse TK, Kimura A, Takiguchi M, Matano T
  • Journal Title: J Virol Volume: 86 Pages: 738-745
  • Peer Reviewed
• [Journal Article] Dominant induction of vaccine antigen-specific cytotoxic T lymphocyte responses after simian immuno deficiency virus challenge (2011)
  • Author(s): Takahara Y, Matsuoka S, Kuwano T, Tsukamoto T, Yamamoto H, Ishii H, Nakasone T, Takeda A, Inoue M, Iida A, Hara H, Shu T, Hasegawa M, Sakawaki H, Horiike M, Miura T, Igarashi T, Naruse TK, Kimura A, Matano T
  • Journal Title: Biochem Biophys Res Commun Volume: 20;408 Pages: 615-619
  • Peer Reviewed
• [Journal Article] Neutralizing antibodies in SIV control ; co-impact with T cells (2010)
  • Author(s): Yamamoto H, Matano T.
  • Journal Title: Vaccine Volume: 28S2 Pages: 13-17
  • Peer Reviewed
• [Journal Article] Neutralizing antibodies in SIV contro1 ; co-impact with T cells (2010)
  • Author(s): Hiroyuki Yamamoto, Tetsuro Matano
  • Journal Title: Vaccine Volume: 28S2 Pages: 13-17
  • Peer Reviewed
• [Presentation] Post-infection passive immunization of SIVmac239-specific, non-neutralizing antibodies does not control virus replication in vivo (2011)
  • Author(s): Nakane T, Matano T, Yamamoto H.
  • Organizer: IUMS 2011(兼第59回日本ウイルス学会学術集会)
  • Place of Presentation: 札幌
  • Year and Date: 2011-09-15
• [Presentation] 中和抗体による感染防御機構:サルエイズモデルにおける解析 (2010)
  • Author(s): 山本浩之
  • Organizer: 第58回日本ウイルス学会学術総会
  • Place of Presentation: 徳島
  • Year and Date: 2010-11-09
• [Presentation] 中和抗体による感染防御機構:サルエイズモデルにおける解析(S12-4) (2010)
  • Author(s): 山本浩之
  • Organizer: 第58回日本ウイルス学会学術集会
  • Place of Presentation: 徳島県 徳島県郷土文化会館
  • Year and Date: 2010-11-09