| Project/Area Number |
22890058
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Orthodontic/Pediatric dentistry
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| Research Institution | Tokyo Medical and Dental University |
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Principal Investigator |
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| Project Period (FY) |
2010 – 2011
|
| Project Status |
Completed (Fiscal Year 2011)
|
| Budget Amount *help |
¥2,977,000 (Direct Cost: ¥2,290,000、Indirect Cost: ¥687,000)
Fiscal Year 2011: ¥1,417,000 (Direct Cost: ¥1,090,000、Indirect Cost: ¥327,000)
Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | 矯正 / 口唇裂・口蓋裂 / エピジェネティック / ヒストン / メチル化 / 環境因子 / 口唇裂 / 顎顔面発生 / 鶏卵 / ヒストンメチル化 / 歯科矯正 |
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| Research Abstract |
The purpose of this study is to identify an involvement of epigenetic changes during cleft lip formation by environmental factors such as retinoic acid. We have accomplished making cleft lip by implanting bead that contained retinoic acid into the nasal pit of E3. 5 chick embryo. Using the same model, we have collected RNA from frontonasal mass and maxillary prominence to see the changes how retinoic acid affect histone metyltransferases or demethyltransferase that covalently modify lysine 9 of histone H3. SETDB1 that tri-methylate histone 3 of lysine 9 was significantly downregulated by treatment of retinoic acid.
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