| Project/Area Number |
22890060
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Virology
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| Research Institution | Yamaguchi University (2011)
Tokyo University of Agriculture and Technology (2010) |
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Principal Investigator |
WATANABE Rie 山口大学, 農学部, 准教授 (50435715)
|
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| Project Period (FY) |
2010 – 2011
|
| Project Status |
Completed (Fiscal Year 2011)
|
| Budget Amount *help |
¥3,042,000 (Direct Cost: ¥2,340,000、Indirect Cost: ¥702,000)
Fiscal Year 2011: ¥1,443,000 (Direct Cost: ¥1,110,000、Indirect Cost: ¥333,000)
Fiscal Year 2010: ¥1,599,000 (Direct Cost: ¥1,230,000、Indirect Cost: ¥369,000)
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| Keywords | インフルエンザウイルス / マトリックスタンパク / 多量体形成 / 粒子内取込み / ウイルス / 感染症 / タンパク質 / インフルエンザ / マトリックス |
|---|
| Research Abstract |
We expressed Flag tagged M1(M1-FL) and HA tagged M1(HA-M1) together in HEK 293T cells and immunoprecipitated using anti-Flag antibody. In the precipitate, HA-M1 was detected in the westernblot analysis with anti-HA antibody. When M1 from filamentous virus A/ Udorn/ 72(H3N2) was analyzed in this system, it was shown that pH of the IP buffer did not affect the formation of complex. On the other hand, M1 from A/ WSN/ 33(H1N1), which forms spherical particle, formed the complex which showed susceptibility to the environmental pH.
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