Research of regulatory B cell role in sclerodermatous GVHD mouse

• Project/Area Number: 22890073
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Dermatology
• Research Institution: Kanazawa University
• Principal Investigator: MATSUSHITA Takashi Kanazawa University, 医学系, 助教 (60432126)
• Project Period (FY): 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥1,599,000 (Direct Cost: ¥1,230,000、Indirect Cost: ¥369,000); Fiscal Year 2010: ¥1,599,000 (Direct Cost: ¥1,230,000、Indirect Cost: ¥369,000)
• Keywords: 強皮症 / B細胞 / IL-10 / 制御性B細胞 / GVHD

Research Abstract

CD19 knockout mouse lack regulatory B cell. Therefore, this mouse is good tool for investigating regulatory B cell. We transferred CD19 knockout B10. D2 bone marrow into Balb/c recipient mice. These mice developed mild sclerodermatous GVHD comparing wildtype mice. While CD19 knockout Balb/c recipient mice which were transferred B10. D2 bone marrow were developed more severe sclerodermatous GVHD than that of wild type mice. These results indicate regulatory B cell is more critical for recipient immune response.

Research Products

• [Journal Article] Use of Serum Clara Cell 16-kDa (CC16) Levels as a Potential Indicator of Active Pulmonary Fibrosis in Systemic Sclerosis. (2011)
  • Author(s): Hasegawa M, Fujimoto M, Hamaguchi Y, Matsushita T, Inoue K, Sato S, Takehara K.
  • Journal Title: J Rheumatol. 38(5) Pages: 877-884
  • Peer Reviewed
• [Journal Article] Serum chemokine and cytokine levelsas indicators of disease activity in patients with systemic sclerosis. (2011)
  • Author(s): Hasegawa M, Fujimoto M, Matsushita T, Hamaguchi Y, Takehara K, Sato S.
  • Journal Title: Clin Rheumatol. in press
  • Peer Reviewed
• [Journal Article] Characterization of a rare IL-10-competent B-cell subset in humans that parallels mouse regulatory B10 cells. (2011)
  • Author(s): Iwata Y, Matsushita T, Horikawa M, Dilillo DJ, Yanaba K, Venturi GM, Szabolcs PM, Bernstein SH, Magro CM, Williams AD, Hall RP, St Clair EW, Tedder TF.
  • Journal Title: Blood. 117(2) Pages: 530-541
  • NAID: 120005756479
  • Peer Reviewed
• [Journal Article] Identifying regulatory B cells (B10 cells) that produce IL-10 in mice. (2011)
  • Author(s): Matsushita T, Tedder TF.
  • Journal Title: Methods Mol Biol. 677 Pages: 99-111
• [Journal Article] Regulatory B cells (B10 cells) and regulatory T cells have independent roles in controlling experimental autoimmune encephalomyelitis initiation and late-phase immunopathogenesis. (2010)
  • Author(s): Matsushita T, Horikawa M, Iwata Y, Tedder TF.
  • Journal Title: J Immunol. 185(4) Pages: 2240-2252
  • Peer Reviewed
• [Presentation] A double-edge sword in B cell depletion therapy for autoimmune disease (2010)
  • Author(s): Takashi Matsushita、Manabu Fujimoto、Thomas F.Tedder, Kazuhiko Takehara
  • Organizer: 第35回日本研究皮膚科学会
  • Place of Presentation: 和歌山県民文化会館(和歌山市)
  • Year and Date: 2010-12-04