| Project/Area Number |
22890078
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Dental engineering/Regenerative dentistry
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| Research Institution | Gifu University |
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Principal Investigator |
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| Project Period (FY) |
2010 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥2,912,000 (Direct Cost: ¥2,240,000、Indirect Cost: ¥672,000)
Fiscal Year 2011: ¥1,391,000 (Direct Cost: ¥1,070,000、Indirect Cost: ¥321,000)
Fiscal Year 2010: ¥1,521,000 (Direct Cost: ¥1,170,000、Indirect Cost: ¥351,000)
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| Keywords | 歯髄細胞 / iPS細胞 / センダイウィルス / DNAマイクロアレイ |
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| Research Abstract |
In this study, we found three new findings. Firstly, human Dental Pulp Cells(DPC) isolated from the tooth of root complete(RC) stage showed much lower reprogramming efficiency than that of DPC isolated from the tooth of crown complete(CC) stage or root forming(RF) stage. This indicated that the efficiency for inducing human iPS cells from DPC lines depend on the developmental stages of the donor tooth. Secondly, we picked up many ES cell-like colonies induced from various DPC lines(isolated from CC, RF, and RC stages) and then evaluated the pluripotency and the differentiation potential. All iPS cell lines tested showed similar levels of pluripotency genes compared to human ES cells, and possessed similar differentiate potency both in vitro and in vivo. We found that the reprogramming efficiency and the developmental stage of donor tooth do not influence the quality of iPS cells. Lastly, we could induce iPS cells by Sendai virus system from DPC lines without the integration of viral transgenes into the host genome. The pluripotency of iPS cells induced by Sendai virus system were equivalent to human ES cells and the iPS cells induced by retroviral system. In addition to this, we found DPC lines can be used as feeder cells for the induction and maintenance of iPS cells.
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