Mechanism of Cardiac Release and Uptake of MicroRNAs:The Role in Cardiac Pathophysiology

• Project/Area Number: 22890090
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Circulatory organs internal medicine
• Research Institution: Kyoto University
• Principal Investigator: NISHI Hitoo Kyoto University, 医学研究科, 研修員 (70583194)
• Project Period (FY): 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥1,885,000 (Direct Cost: ¥1,450,000、Indirect Cost: ¥435,000); Fiscal Year 2010: ¥1,885,000 (Direct Cost: ¥1,450,000、Indirect Cost: ¥435,000)
• Keywords: MicroRNA / エクソソーム / 虚血性心疾患 / microRNA

Research Abstract

Serum levels of cardiac specific microRNAs in patients with ischemic heart disease were quantified by real-time PCR and consequently, miR-1 and miR-133a were found to be up-regulated. It was also demonstrated that A23187 could increase microRNA levels in the culture medium of H9C2 rat myoblast cells in a dose-dependent manner, and microRNAs contained in exosomes might be incorporated into cells and function in the recipient cells.

Research Products

• [Presentation] マイクロRNA-1とマイクロRNA-133aは急性冠症候群患者の有用な早期診断バイオマーカーである (2010)
  • Author(s): 桑原康秀、尾野亘、堀江貴裕、西仁勇、木下美菜子、渡邉真、中島康弘、長央和也、長谷川浩二、小島洋児、北徹、木村剛
  • Organizer: アメリカ心臓学会
  • Place of Presentation: シカゴ
• [Presentation] MicroRNA-1 and microRNA-133a are useful as early diagnostic biomarkers in patients with acute coronary syndrome. (2010)
  • Author(s): Kuwabara Y, Ono K, Horie T, Nishi H, Kinoshita M, Watanabe S, Nakashima Y, Nagao K, Kojima Y, Kita T, Kimura T
  • Organizer: American Heart Association Annual Scientific Sessions 2010
  • Place of Presentation: Chicago, Illinois, USA