Project/Area Number |
22890103
|
Research Category |
Grant-in-Aid for Research Activity Start-up
|
Allocation Type | Single-year Grants |
Research Field |
Circulatory organs internal medicine
|
Research Institution | Osaka University |
Principal Investigator |
TSUKAMOTO Osamu 大阪大学, 医学系・研究科, 特任研究員 (80589151)
|
Project Period (FY) |
2010 – 2011
|
Project Status |
Completed (Fiscal Year 2011)
|
Budget Amount *help |
¥3,068,000 (Direct Cost: ¥2,360,000、Indirect Cost: ¥708,000)
Fiscal Year 2011: ¥1,469,000 (Direct Cost: ¥1,130,000、Indirect Cost: ¥339,000)
Fiscal Year 2010: ¥1,599,000 (Direct Cost: ¥1,230,000、Indirect Cost: ¥369,000)
|
Keywords | c-myc / 転写複合体 / 心筋細胞特異的遺伝子発現制御 / クロスリンク反応 |
Research Abstract |
We found that the expression of one gene is down-regulated in cardiomyocytes while up-regulated in HUVEC or smooth muscle cells by c-myc. To clarify this cardiomyocyte-specific transcriptional mechanism by c-myc, we developed the nuclear extraction method that can obtain structurally-intact transcription complexes binding with high-affinity to their specific DNA sequences. Next, we narrowed the range of putative binding site of c-myc transcription complexes in promoter region of the gene by using both comparative genomics approach and transactivation assay. We performed DNA affinity chromatography using oligonucleotides containing the binding site of c-myc transcription complex as bait and nuclear extracts from cardiomyocytes or HUVEC. Using a high-sensitive LC-MS/ MS technology, we identified cardiomyocyte-specific components in c-myc transcription complexes.
|