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Identification of cardiomyocyte-specific transcriptional mechanism of c-myc

Research Project

Project/Area Number 22890103
Research Category

Grant-in-Aid for Research Activity Start-up

Allocation TypeSingle-year Grants
Research Field Circulatory organs internal medicine
Research InstitutionOsaka University

Principal Investigator

TSUKAMOTO Osamu  大阪大学, 医学系・研究科, 特任研究員 (80589151)

Project Period (FY) 2010 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥3,068,000 (Direct Cost: ¥2,360,000、Indirect Cost: ¥708,000)
Fiscal Year 2011: ¥1,469,000 (Direct Cost: ¥1,130,000、Indirect Cost: ¥339,000)
Fiscal Year 2010: ¥1,599,000 (Direct Cost: ¥1,230,000、Indirect Cost: ¥369,000)
Keywordsc-myc / 転写複合体 / 心筋細胞特異的遺伝子発現制御 / クロスリンク反応
Research Abstract

We found that the expression of one gene is down-regulated in cardiomyocytes while up-regulated in HUVEC or smooth muscle cells by c-myc. To clarify this cardiomyocyte-specific transcriptional mechanism by c-myc, we developed the nuclear extraction method that can obtain structurally-intact transcription complexes binding with high-affinity to their specific DNA sequences. Next, we narrowed the range of putative binding site of c-myc transcription complexes in promoter region of the gene by using both comparative genomics approach and transactivation assay. We performed DNA affinity chromatography using oligonucleotides containing the binding site of c-myc transcription complex as bait and nuclear extracts from cardiomyocytes or HUVEC. Using a high-sensitive LC-MS/ MS technology, we identified cardiomyocyte-specific components in c-myc transcription complexes.

Report

(3 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • Research Products

    (12 results)

All 2012 2011 2010

All Journal Article (12 results) (of which Peer Reviewed: 12 results)

  • [Journal Article] It is Time to Reconsider the Cardiovascular Protection Afforded by RAAS Blockade-Overview of RAAS Systems2012

    • Author(s)
      Tsukamoto O and Kitakaze M.
    • Journal Title

      Cardiovasc Drugs Ther

      Volume: (in press)

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] It is Time to Reconsider the Cardiovascular Protection Afforded by RAAS Blockade-Overview of RAAS Systems2011

    • Author(s)
      Osamu Tsukamoto
    • Journal Title

      Cardiovascular Drugs and Therapy

      Volume: 27 Issue: 2 Pages: 133-138

    • DOI

      10.1007/s10557-011-6361-1

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Differential regulation of proteasome function in isoproterenol-induced cardiac hypertrophy2010

    • Author(s)
      Drews O, Tsukamoto O, Liem D, Streicher J, Wang Y, Ping P.
    • Journal Title

      Circ Res

      Volume: 107 Pages: 1094-1101

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Isoform-specific intermolecular disulfide bond formation of heterochromatin protein 1(HP1)2010

    • Author(s)
      Higo S, Asano Y, Kato H, Yamazaki S, Nakano A, Tsukamoto O, Seguchi O, Asai M, Asakura M, Asanuma H, Sanada S, Minamino T, Komuro I, Kitakaze M, Takashima S.
    • Journal Title

      J Biol Chem

      Volume: 285 Pages: 31337-31347

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Ablation of C/ EBP Homologous Protein Attenuates Endoplasmic Reticulum-Mediated Apoptosis and Cardiac Dysfunction Induced by Pressure Overload2010

    • Author(s)
      Fu HY, Okada K, Liao Y, Tsukamoto O, Isomura T, Asai M, Sawada T, Okuda K, Asano Y, Sanada S, Asanuma H, Asakura M, Takashima S, Komuro I, Kitakaze M, Minamino T.
    • Journal Title

      Circulation

      Volume: 122 Pages: 361-369

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] What is the role of ER stress in the heart? Introduction and series overview2010

    • Author(s)
      Kitakaze M and Tsukamoto O.
    • Journal Title

      Circ Res

      Volume: 208 Pages: 15-18

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] X-box binding protein 1 regulates brain natriuretic peptide through a novel AP1/ CRE-like element in cardiomyocytes2010

    • Author(s)
      Sawada T, Minamino T, Fu HY, Asai M, Okuda K, Isomura T, Yamazaki S, Asano Y, Okada K, Tsukamoto O, Sanada S, Asanuma H, Asakura M, Takashima S, Kitakaze M, Komuro I.
    • Journal Title

      J Mol Cell Cardiol

      Volume: 48 Pages: 1280-1289

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Functional alterations of cardiac proteasomes under physiological and pathological conditions2010

    • Author(s)
      Tsukamoto O, Minamino T, Kitakaze M.
    • Journal Title

      Cardiovasc Res

      Volume: 85 Pages: 339-346

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Differential regulation of proteasome function in isoproterenol-induced cardiac hypertrophy.2010

    • Author(s)
      Drews O, Tsukamoto O
    • Journal Title

      Circulation Research

      Volume: 107 Pages: 1094-1101

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Isoform-specific intermolecular disulfide bond formation of heterochromatin protein 1.2010

    • Author(s)
      Higo S, Tsukamoto O
    • Journal Title

      Journal of Biological Chemistry

      Volume: 285 Pages: 361-369

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Ablation of C/EBP homologous protein attenuates ER-mediated apoptosis and cardiac dysfunction induced by pressure overload.2010

    • Author(s)
      Fu HY, Tsukamoto O
    • Journal Title

      Circulation

      Volume: 122 Pages: 361-369

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] What is the role of ER stress? Introduciton and series overview.2010

    • Author(s)
      Kitakaze M, Tsukamoto O
    • Journal Title

      Circulation Research

      Volume: 107 Pages: 15-18

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed

URL: 

Published: 2010-08-27   Modified: 2016-04-21  

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