• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

The regulation of cytoskeletal dynamics in endothelial cells during acute inflammation

Research Project

Project/Area Number 22890104
Research Category

Grant-in-Aid for Research Activity Start-up

Allocation TypeSingle-year Grants
Research Field Circulatory organs internal medicine
Research InstitutionOsaka University

Principal Investigator

KATO Hisakazu  大阪大学, 大学院・医学系研究科, 特任研究員 (30589312)

Project Period (FY) 2010 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥3,068,000 (Direct Cost: ¥2,360,000、Indirect Cost: ¥708,000)
Fiscal Year 2011: ¥1,469,000 (Direct Cost: ¥1,130,000、Indirect Cost: ¥339,000)
Fiscal Year 2010: ¥1,599,000 (Direct Cost: ¥1,230,000、Indirect Cost: ¥369,000)
Keywords組織・細胞 / 生体分子 / 循環器・高血圧 / 血管内皮細胞 / タンパク質キナーゼ / タンパク質脱リン酸化酵素 / 炎症性サイトカイン / 細胞遊走
Research Abstract

In this research project, I examined the role of cytokine-induced kinase NUAK2 in endothelial cells. When NUAK2 was knocked down in endothelial cells, loss of actin stress fiber and decrease in phosphorylation of myosin light chain were observed. Furthermore, I identified several auto-phosphorylation sites in NUAK2, which involved in activation of its kinase. I injected antisense morpholino against NUAK2 into the zebrafish allowing live imaging of blood vessels, and they showed lethal phenotypes. These data suggest that NUAK2-MYPT1 axis involves in the regulation of cytoskeletal dynamics in vitro and in vivo.

Report

(3 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • Research Products

    (6 results)

All 2012 2011 2010

All Journal Article (6 results) (of which Peer Reviewed: 6 results)

  • [Journal Article] Development of anti-HB-EGF immunoliposomes for the treatment of breast cancer.2012

    • Author(s)
      K. Nishikawa, T. Asai, H. Shigematsu, K. Shimizu, H. Kato, Y. Asano, S. Takashima, E. Mekada, N. Oku, T. Minamino
    • Journal Title

      J. Control. Release

      Volume: 160 Issue: 2 Pages: 274-280

    • DOI

      10.1016/j.jconrel.2011.10.010

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Development of anti-HB-EGF immunoliposomes for the treatment of breast cancer2011

    • Author(s)
      Nishikawa K, Asai T, Shigematsu H, Shimizu K, Kato H, Asano Y, Takashima S, Mekada E, Oku N, Minamino T
    • Journal Title

      J Control Release

      Volume: (in press)

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Isoform-specific intermolecular disulfide bond formation of heterochromatin protein 1(HP1)2010

    • Author(s)
      Higo S, Asano Y, Kato H, Yamazaki S, Nakano A, Tsukamoto O, Seguchi O, Asai M, Asakura M, Asanuma H, Sanada S, Minamino T, Komuro I, Kitakaze M, Takashima S
    • Journal Title

      J Biol Chem

      Volume: 285 Pages: 31337-47

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] AMPK controls the speed of microtubule polymerization and directional cell migration through CLIP-170 phosphorylation2010

    • Author(s)
      Nakano A, Kato H, Watanabe T, Min KD, Yamazaki S, Asano Y, Seguchi O, Higo S, Shintani Y, Asanuma H, Asakura M, Minamino T, Kaibuchi K, Mochizuki N, Kitakaze M, Takashima S
    • Journal Title

      Nat Cell Biol

      Volume: 12 Pages: 583-90

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] AMPK controls the speed of microtubule polymerization and directional cell migration through CLIP-170 phosphorylation.2010

    • Author(s)
      中野敦、加藤久和
    • Journal Title

      Nature Cell Biology

      Volume: 12 Pages: 583-590

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Isoform-specific intermolecular disulfide bond formation of heterochromat in protein 1 (HP1).2010

    • Author(s)
      肥後修一朗、加藤久和
    • Journal Title

      Journal of Biological Chemistry

      Volume: 285 Pages: 31337-31347

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed

URL: 

Published: 2010-08-27   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi