Creation of polyclonal antibody of secreted protein SEPINE2, which is expressed in testicular cancer cell line with high metastatic potential. How does it work in testicular cancer metastasis?

• Project/Area Number: 22890108
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Urology
• Research Institution: Osaka University
• Principal Investigator: NAGAHARA Akira 大阪大学, 医学部附属病院, 医員 (90588774)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥3,068,000 (Direct Cost: ¥2,360,000、Indirect Cost: ¥708,000); Fiscal Year 2011: ¥1,469,000 (Direct Cost: ¥1,130,000、Indirect Cost: ¥339,000); Fiscal Year 2010: ¥1,599,000 (Direct Cost: ¥1,230,000、Indirect Cost: ¥369,000)
• Keywords: 精巣腫瘍 / 転移 / SERPINE2 / SEPINE2

Research Abstract

We tried to create a polyclonal antibody of secreted protein SERPINE2 that we had reported before and to elucidate how it work in testicular cancer metastasis. Although we established stable SERPINE-expressed testicular cancer cell line, we couldn' t get evidence that SERPINE2 was metastatic promoter in vivo and mRNA expression analysis in clinical samples. And we regretted to create the antibody of SERPINE2.