| Project/Area Number |
22890110
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Orthodontic/Pediatric dentistry
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| Research Institution | Osaka University |
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Principal Investigator |
MICHIKAMI Ikumi 大阪大学, 大学院・歯学研究科, リサーチアシスタント (80589771)
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| Project Period (FY) |
2010 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥2,977,000 (Direct Cost: ¥2,290,000、Indirect Cost: ¥687,000)
Fiscal Year 2011: ¥1,417,000 (Direct Cost: ¥1,090,000、Indirect Cost: ¥327,000)
Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | 骨芽細胞 / 転写因子 / ジンクフィンガー / マウス / KLF / Runx2 / オステオカルシン / 骨形成 / 石灰化 / Wnt |
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| Research Abstract |
Kruppel-like factor 4(KLF4/ GKLF/ EZF) is a zinc finger type of transcription factor highly expressed in the skin, intestine, testis, lung and bone. Its role in bone cells is unknown. To investigate the possible roles played by Klf4 during skeletal development, we generated transgenicmice expressing Klf4 under mouse type I collagen regulatory sequence. Transgenic mice showed delayed formation of the calvarial bones ; and over-expressing Klf4 in primary mouse calvarial osteoblasts in culture resulted in repression of mineralization indicating that this regulation of Klf4 is through an osteoblast-autonomous effect. Surprisingly, long bones of the transgenicmice exhibited delayed marrowcavity formation. Our results demonstrate that Klf4 regulates normal skeletal development through coordinating the differentiation and migration of osteoblasts, chondrocytes, vascular endothelial cells and osteoclasts.
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