Development of the antibody drug production system using a highly efficient artificial chromosome vector.
| Project/Area Number |
22890114
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Drug development chemistry
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| Research Institution | Tottori University |
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Principal Investigator |
NISHIDA Tadashi 鳥取大学, 染色体工学研究センター, プロジェクト研究員 (50588556)
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| Co-Investigator(Renkei-kenkyūsha) |
OSHIMURA Mitsuo 鳥取大学, 大学院・医学系研究科, 教授 (20111619)
OHBAYASHI Tetsuya 鳥取大学, 生命機能研究支援センタ, 准教授 (80348804)
KAZUKI Yasuhiro 鳥取大学, 大学院・医学系研究科, 助教 (90403401)
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| Project Period (FY) |
2010 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥2,951,000 (Direct Cost: ¥2,270,000、Indirect Cost: ¥681,000)
Fiscal Year 2011: ¥1,352,000 (Direct Cost: ¥1,040,000、Indirect Cost: ¥312,000)
Fiscal Year 2010: ¥1,599,000 (Direct Cost: ¥1,230,000、Indirect Cost: ¥369,000)
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| Keywords | 抗体医薬 / 人工染色体 / 抗体医薬産生 |
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| Research Abstract |
In this study, we tried development of the technology which can increase the amount of antibody production and stabilize the quality of antibody drug production cells using the artificial chromosome vector(which enable long-term stable expression of transgenes) to reduce the cost for antibody-drug production in industry.
When we introduced the medicine candidate gene into the artificial chromosome vector and measured the purpose protein in a culture medium, the amount of production increased to the number of transgenes.
For the future, we continue the study to increase the amount of production using gene amplification system.
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Report
(3 results)
Research Products
(13 results)
