| Project/Area Number |
22890136
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Parasitology (including Sanitary zoology)
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| Research Institution | Saga University |
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Principal Investigator |
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| Project Period (FY) |
2010 – 2011
|
| Project Status |
Completed (Fiscal Year 2011)
|
| Budget Amount *help |
¥3,146,000 (Direct Cost: ¥2,420,000、Indirect Cost: ¥726,000)
Fiscal Year 2011: ¥1,508,000 (Direct Cost: ¥1,160,000、Indirect Cost: ¥348,000)
Fiscal Year 2010: ¥1,638,000 (Direct Cost: ¥1,260,000、Indirect Cost: ¥378,000)
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| Keywords | 赤痢アメーバ / マイトソーム / 硫酸活性化経路 / 含硫脂質 / chlorate / 宿主免疫応答 / 寄生虫感染症 |
|---|
| Research Abstract |
Although the sulfate activation pathway is the major function in E. histolytica mitosomes, their physiological role remains unknown. In this study, I examined the phenotypes of the parasites in which genes involved in the mitosome functions were suppressed by gene silencing, and showed that sulfate activation in mitosomes is important for sulfolipid synthesis and cell proliferation. Furthermore, I found that chlorate(ClO3-) inhibited cell growth of E. histolytica(IC50=10. 5 mM). The importance and topology of the components in E. histolytica mitosomes reinforce the notion that they are not" rudimentary" or" residual" mitochondria, but represent a uniquely evolved crucial organelle in E. histolytica.
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