Analysis of ubiquitination pathway to intracytoplasmic bacteria

• Project/Area Number: 22890154
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Bacteriology (including Mycology)
• Research Institution: University of Shizuoka
• Principal Investigator: YOSHIKAWA Yuko 静岡県立大学, 食品栄養科学部, 助教 (00580523)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥3,146,000 (Direct Cost: ¥2,420,000、Indirect Cost: ¥726,000); Fiscal Year 2011: ¥1,508,000 (Direct Cost: ¥1,160,000、Indirect Cost: ¥348,000); Fiscal Year 2010: ¥1,638,000 (Direct Cost: ¥1,260,000、Indirect Cost: ¥378,000)
• Keywords: 細菌 / 感染症 / リステリア / オートファジー / ユビキチン

Research Abstract

Ubiquitinated ActA-deficient Listeria monocytogenes(. actA) were recognized by autophagy. To elucidate the mechanism of Listeria ubiquitination, we attempted to explore the bacterial ubiquitinized molecules and the host ubiquitination factors. As a result, two candidates of bacterial ubiquitinated molecules and four candidates involved in host ubiquitination system were identified by LC-MS/MS analysis. The recombinant proteins of Listeria candidate moleculesexamined by in vitro ubiquitination assay, and one of the two candidates inListeria wa weres received ubiquitination directly.

Research Products

• [Journal Article] Detection and Characterization of p44/msp2 Transcript Variants of Anaplasma phagocytophilum from Naturally Infected Ticks and Wild Deer in Japan (2012)
  • Author(s): Gaowa、Wuritu、Wu D.、Yoshikawa Y.、Ohashi N.、Kawamori F.、Sugiyama K.、Ohtake M.、Ohashi M.、Yamamoto S.、Kitano T.、Takada N. and Kawabata H
  • Journal Title: Jpn. J. Infect. Dis Volume: 65 Pages: 79-83
  • Peer Reviewed
• [Journal Article] Detection and characterization of p44/msp2 transcript variants of Anaplasma phagocytophilum from naturally infected ticks and wild deer in Japan (2012)
  • Author(s): Gaowa
  • Journal Title: Japanese Journal of Infectious Diseases Volume: VOL.65 Pages: 79-83
  • Peer Reviewed
• [Journal Article] Manipulation of autophagy by bacteria for their own benefit (2011)
  • Author(s): Ogawa M.、Mimuro H.、Yoshikawa Y.、Ashida H. and Sasakawa C
  • Journal Title: Microbiology and Immunology Volume: 55 Pages: 459-471
  • NAID: 10031121491
  • Peer Reviewed
• [Journal Article] The role of a Tecpr1-dependent selective autophagy pathway in targeting bacterial pathogens (2011)
  • Author(s): Ogawa M.、Yoshikawa Y.、Kobayashi T.、Mimuro H.、Fukumatsu M.、Kiga K.、Piao Z.、Yoshida M.、Kakuta S.、Koyama T.、Goto Y.、Nagatake T.、Igarashi O.、Nagai S.、Kiyono H.、Iwakura Y.、Kawalec M.、Jean-Marc Reichhart J. M. and Sasakawa C
  • Journal Title: Cell Host & Microbe Volume: 9 Pages: 376-389
  • Peer Reviewed
• [Journal Article] A survey ofβ-lactamase-producing Escherichia coli in farm animals and raw retail meat in Shizuoka prefecture, Japan (2011)
  • Author(s): Hiroi M.、Harada T.、Kawamori F.、Takahashi N.、Kanda T.、Sugiyama K.、Masuda T.、Yoshikawa Y. and Ohashi
  • Journal Title: Jpn. J. Infect. Dis Volume: 64 Pages: 153-155
  • Peer Reviewed
• [Journal Article] Bacterial interactions with the host epithelium (2011)
  • Author(s): Kim M.、Ashida H.、Ogawa M.、Yoshikawa Y.、Mimuro H. and Sasakawa C
  • Journal Title: Cell Host & Microbe Volume: 8 Pages: 20-35
  • Peer Reviewed
• [Journal Article] A tecpr1-dependent selective autophagy pathway targets bacterial pathogens (2011)
  • Author(s): Kawalec M, Reichhart JM, Sasakawa C
  • Journal Title: Cell Host Microbe Volume: 9 Issue: 5 Pages: 376-389
  • DOI: 10.1016/j.chom.2011.04.010
  • Peer Reviewed
• [Journal Article] Manipulation of autophagy by bacteria for their own benefit (2011)
  • Author(s): Ogawa M.
  • Journal Title: Microbiology and Immunology Volume: VOL.55 Issue: 7 Pages: 459-471
  • DOI: 10.1111/j.1348-0421.2011.00343.x
  • Peer Reviewed
• [Journal Article] A survey of β-lactamase-producing Escherichia coli in farm animals and raw retail meat in Shizuoka prefecture, Japan (2011)
  • Author(s): Hiroi M.
  • Journal Title: Japanese Journal of Infectious Diseases Volume: VOL.64 Pages: 153-155
  • Peer Reviewed
• [Journal Article] Novel concentration method for detection of norovirus and sapovirus from water using minute particles of amorphous calcium phosphate (2011)
  • Author(s): Shinohara M.
  • Journal Title: Journal of Medical Microbiology Volume: VOL.60 Pages: 780-786
  • Peer Reviewed
• [Journal Article] Autophagy targeting of Listeria monocytogenes and the bacterial countermeasure. (2011)
  • Author(s): Ogawa, M., Yoshikawa, Y., Mimuro, H., Hain, T., Chakraborty, T., Sasakawa, C.
  • Journal Title: Autophagy Volume: 7 Pages: 310-314
  • Peer Reviewed
• [Journal Article] Bacterial interactions with the host epithelium. (2010)
  • Author(s): Kim, M., Ashida, H., Ogawa, M., Yoshikawa, Y., Mimuro, H., Sasakawa, C.
  • Journal Title: Cell Host & Microbe Volume: 8 Pages: 20-35
  • Peer Reviewed
• [Journal Article] Novel concentration method for detection of norovirus and sapovirus from water using minute particles of amorphous calcium phosphate
  • Author(s): Shinohara M.、Uchida K.、Shimada S. I.、Tomioka K.、Suzuki N.、Minegishi T.、Kawahashi S.、Yoshikawa Y. and Ohashi N
  • Journal Title: J. Med. Microbiol Volume: 60 Pages: 780-786
  • Peer Reviewed
• [Journal Article] Autophagy targeting of Listeria monocytogenes and the bacterial countermeasure
  • Author(s): Ogawa M.、Yoshikawa Y.、Mimuro H.、Hain T.、Chakraborty T. and Sasakawa, C
  • Journal Title: Autophagy Volume: 7 Pages: 310-3141
  • Peer Reviewed
• [Presentation] Identification of lactic acid bacteria in wasabi-dzuke and molecular analysis of human fecal microflora in the food intake (2011)
  • Author(s): Yamazaki M.、Masuda R.、Tanaka M.、Serizawa T.、Murakami T.、Kobayashi Y.、Yoshikawa Y.、Masuda H.、Hisada T. and Ohashi N
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜(横浜)
  • Year and Date: 2011-12-14
• [Presentation] Identification of lactic acid bacteria in wasabi-dzuke and molecular analysis of human fecal microflora in the food intake (2011)
  • Author(s): Yamazaki M.
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2011-12-14
• [Presentation] 静岡県の野性シカが保有するリケッチア関連細菌群の解析 (2011)
  • Author(s): 呉東興、烏日図、高娃、川森文彦、吉川悠子、大橋典男
  • Organizer: 第19回ダニと疾患のインタフェースに関するセミナー(SADI)つつがの里大会
  • Place of Presentation: 川・森文化交流センター(広島)
  • Year and Date: 2011-11-05
• [Presentation] 静岡県の野性シカが保有するリケッチア関連細菌群の解析 (2011)
  • Author(s): 呉東興
  • Organizer: 第19回ダニと疾患のインタフェースに関するセミナー(SADI)つつがの里大会
  • Place of Presentation: 川・森文化交流センター
  • Year and Date: 2011-11-05
• [Presentation] 非晶性リン酸カルシウム微粒子を用いた食品からのウイルス検出法 (2011)
  • Author(s): 篠原美千代、富岡恭子、峯岸俊貴、鈴木典子、内田和江、島田慎一、河橋幸恵、岸本剛、吉川悠子、大橋典男、野田衛
  • Organizer: 第32回日本食品微生物学会学術総会
  • Place of Presentation: タワーホール船堀(東京)
  • Year and Date: 2011-10-07
• [Presentation] 非晶性リン酸カルシウム微粒子を用いた食品からのウイルス検出法 (2011)
  • Author(s): 篠原美千代
  • Organizer: 第32回日本食品微生物学会学術総会
  • Place of Presentation: タワーホール船堀
  • Year and Date: 2011-10-07
• [Presentation] Human infection with Anaplasma phagocytophilum in Japan (2011)
  • Author(s): 高娃、烏日図、呉東興、古川英嗣、吉川悠子、川森文彦、大橋典男
  • Organizer: 第94回日本細菌学会関東支部総会
  • Place of Presentation: 北里大学白金キャンパス(東京)
  • Year and Date: 2011-10-06
• [Presentation] Human infection with Anaplasma phagocytophillum in-Japan (2011)
  • Author(s): 高娃
  • Organizer: 第94回日本細菌学会関東支部総会
  • Place of Presentation: 北里大学白金キャンパス本館大会議室
  • Year and Date: 2011-10-06
• [Presentation] Development of new method for classification of lactic acid bacteria using whole-cell MALDI-TOF MS (2011)
  • Author(s): Murakami T.、Tanaka M.、Masuda R., Yamazaki M.、Kobayashi Y.、Serizawa T.、Yoshikawa Y. and Ohashi N
  • Organizer: International Union of Microbiological Societies 2011 Congress
  • Place of Presentation: 札幌コンベンションセンター(札幌)
  • Year and Date: 2011-09-09
• [Presentation] Evaluation of Wasabi-dzuke(sake lees containing wasabi) as human probiotics (2011)
  • Author(s): Yamazaki M.、Masuda R.、Tanaka M.、Serizawa T.、Murakami T.、Kobayashi Y.、Yoshikawa Y.、Masuda H.、Hisada T. and Ohashi N
  • Organizer: International Union of Microbiological Societies 2011 Congress
  • Place of Presentation: 札幌コンベンションセンター(札幌)
  • Year and Date: 2011-09-09
• [Presentation] Molecular epidemiological study of Rickettsiales bacteria in wild deer and mice in Shizuoka prefecture, Japan (2011)
  • Author(s): Wuritu、Wu D.、Yoshikawa Y.、Furukawa E.、Murakami T.、Minato C.、Kawamori F.、Ohtake M.、Ohashi M. and Ohashi N
  • Organizer: International Union of Microbiological Societies 2011 Congress
  • Place of Presentation: 札幌コンベンションセンター(札幌)
  • Year and Date: 2011-09-09
• [Presentation] Molecular detection of Rickettsiales bacteria from ticks in western Japan (2011)
  • Author(s): Gaowa、Wuritu、Aochi M.、Kawamori F.、Yoshikawa Y. and Ohashi N
  • Organizer: International Union of Microbiological Societies 2011 Congress
  • Place of Presentation: 札幌コンベンションセンター(札幌)
  • Year and Date: 2011-09-09
• [Presentation] Molecular detection of Rickettsiales bacteria from ticks in western Japan (2011)
  • Author(s): Gaowa
  • Organizer: International Union of Microbiological Societies 2011 Congress
  • Place of Presentation: 札幌コンベンションセンター
  • Year and Date: 2011-09-09
• [Presentation] Molecular epidemiological study of Rickettsiales bacteria in wild deer and mice in Shizuoka prefecture, Japan (2011)
  • Author(s): Yoshikawa Y.
  • Organizer: International Union of Microbiological Societies 2011 Congress
  • Place of Presentation: 札幌コンベンションセンター
  • Year and Date: 2011-09-09
• [Presentation] Evaluation of Wasabi-duke (sake lees containing wasabi) as human probiotics (2011)
  • Author(s): Yamazaki M.
  • Organizer: International Union of Microbiological Societies 2011 Congress
  • Place of Presentation: 札幌コンベンションセンター
  • Year and Date: 2011-09-09
• [Presentation] Development of new method for classification of lactic acid bacteria using whole-cell MALDI-TOF MS (2011)
  • Author(s): Murakami T.
  • Organizer: International Union of Microbiological Societies 2011 Congress
  • Place of Presentation: 札幌コンベンションセンター
  • Year and Date: 2011-09-09
• [Presentation] わさび漬けに存在する乳酸産生菌群の解析 (2011)
  • Author(s): 山崎芽生、芹澤委子、増田竜也、村上拓也、田中実穂、吉川悠子、久田貴義、富田順子、増田英樹、大橋典男
  • Organizer: 日本乳酸菌学会2011年度大会
  • Place of Presentation: 関西大学千里山キャンパス(大阪)
  • Year and Date: 2011-07-11
• [Presentation] 炎症性腸疾患の緩和に有効な乳酸菌探索のためのin vivoイメージング法の検討 (2011)
  • Author(s): 田中実穂、吉川悠子、村上拓也、山崎芽生、芹澤委子、増田竜也、小林靖尚、大橋典男
  • Organizer: 日本乳酸菌学会2011年度大会
  • Place of Presentation: 関西大学千里山キャンパス(大阪)
  • Year and Date: 2011-07-11
• [Presentation] MALDI-TOF MSを用いた乳酸菌種の新規同定法に関する研究 (2011)
  • Author(s): 村上拓也、田中実穂、増田竜也、山崎芽生、渡邉茉純、芹澤委子、吉川悠子、大橋典男
  • Organizer: 日本乳酸菌学会2011年度大会
  • Place of Presentation: 関西大学千里山キャンパス(大阪)
  • Year and Date: 2011-07-11
• [Presentation] MALDI-TOF MSを用いた乳酸菌種の新規同定法に関する研究 (2011)
  • Author(s): 村上拓也
  • Organizer: 日本乳酸菌学会2011年度大会
  • Place of Presentation: 関西大学千里山キャンパス100周年記念会館
  • Year and Date: 2011-07-11
• [Presentation] 炎症性腸疾患の緩和に有効な乳酸菌探索のためのin vivoイメージング法の検討 (2011)
  • Author(s): 大橋典男
  • Organizer: 日本乳酸菌学会2011年度大会
  • Place of Presentation: 関西大学千里山キャンパス100周年記念会館
  • Year and Date: 2011-07-11
• [Presentation] わさび漬けに存在する乳酸産生菌群の解析 (2011)
  • Author(s): 山崎芽生
  • Organizer: 日本乳酸菌学会2011年度大会
  • Place of Presentation: 関西大学千里山キャンパス100周年記念会館
  • Year and Date: 2011-07-11
• [Presentation] Listeria monocytogenesのオートファジー認識システムからの回避機構の解明 (2010)
  • Author(s): 吉川悠子、大橋典男
  • Organizer: 第18回ダニと疾患のインタフェースに関するセミナー(SADI)トキの里大会
  • Place of Presentation: トキ交流会館(新潟)
  • Year and Date: 2010-06-13
• [Presentation] Listeria monocytogenesのオートファジー認識システムからの回避機構の解明 (2010)
  • Author(s): 吉川悠子、大橋典男
  • Organizer: 第18回ダニと疾患のインタフェースに関するセミナーSADIトキの里大会
  • Place of Presentation: トキ交流会館
  • Year and Date: 2010-06-13