Effects of BMP2 and FGF2 on the odontoblast differentiation and the induction methods of expression of cell function for the regeneration of dental pulp.

• Project/Area Number: 22890167
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Conservative dentistry
• Research Institution: Kyushu Dental College
• Principal Investigator: WASHIO Ayako 九州歯科大学, 歯学部歯学科, 助教 (10582786)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥2,977,000 (Direct Cost: ¥2,290,000、Indirect Cost: ¥687,000); Fiscal Year 2011: ¥1,417,000 (Direct Cost: ¥1,090,000、Indirect Cost: ¥327,000); Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 象牙質・歯髄複合体 / 再生医療

Research Abstract

We examined the effects of Bone Morphogenic Protein-2(BMP-2) and Fibroblast Growth Factor-2(FGF-2), on the odontoblast differentiation of KN-3 cells, a cell line that we established from dental pulp. Our results indicate that BMP-2 and FGF-2 play critical roles in induction of the odontoblast properties of KN-3 cells via activation of Smad and ERK signaling pathways, respectively. We could have useful knowledge about the establishment of wound healing and local regeneration of dentin-pulp complex.

Research Products

• [Journal Article] Possible Involvement of Smad Signaling Pathways in Induction of Odontoblastic Properties in KN-3 Cells by Bone Morphogenetic Protein-2, a Growth Factor to Induce Dentin Regeneration (2012)
  • Author(s): Ayako Washio、Chiaki Kitamura、Takahiko Morotomi
  • Journal Title: International Journal of Dentistry Volume: VOL. 2012 Pages: 1-6
  • DOI: 10.1155/2012/258469
  • Peer Reviewed
• [Journal Article] Local regeneration of dentin-pulp complex using controlled release of FGF-2 and naturally derived sponge-like scaffolds. (2012)
  • Author(s): Kitamura C, Nishihara T, Terashita M, Tabata Y, Washio A
  • Journal Title: International Journal of Dentistry Volume: volume2012 Pages: 8-8
  • DOI: 10.1155/2012/190561
  • Peer Reviewed
• [Journal Article] Regeneration approach for dental pulp and periapical tissues with growth factors, biomaterials, and laser irradiation. Special issue "Polymers for Oro-Dental and Cranio-Maxillo-Facial Applications" (2011)
  • Author(s): Chiaki Kitamura、Tatusji Nishihara、Ayako Washio
  • Journal Title: Polymers Volume: 3(4) Pages: 1776-1793
• [Presentation] FGF-2は象牙芽細胞様細胞の形態変化を誘導する (2011)
  • Author(s): 鷲尾絢子
  • Organizer: 第9回日本再生歯科医学学術大会・総会
  • Year and Date: 2011-09-10
• [Presentation] 象牙芽細胞分化に及ぼすFGF-2の影響 (2011)
  • Author(s): 鷲尾絢子
  • Organizer: 第32回日本歯内療法学会学術大会
• [Presentation] FGF-2が象牙芽細胞様細胞に及ぼす影響 (2011)
  • Author(s): 鷲尾絢子
  • Organizer: 第134回日本歯科保存学会
• [Presentation] 象牙芽細胞様細胞の分化におけるFGF-2の影響 (2011)
  • Author(s): 鷲尾絢子
  • Organizer: 第71回九州歯科学会総会
• [Presentation] 象牙芽細胞様細胞の分化におけるBMP-2の影響 (2010)
  • Author(s): 鷲尾絢子
  • Organizer: 九州歯科学会総会記念学会
  • Place of Presentation: 九州歯科大学(北九州)