| Project/Area Number |
22890178
|
|---|
| Research Category |
Grant-in-Aid for Research Activity Start-up
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
Orthopaedic surgery
|
|---|
| Research Institution | Keio University |
|---|
Principal Investigator |
OCHI Hiroki 慶應義塾大学, 医学部, 特任助教 (30582283)
|
|---|
| Project Period (FY) |
2010 – 2011
|
| Project Status |
Completed (Fiscal Year 2011)
|
| Budget Amount *help |
¥3,068,000 (Direct Cost: ¥2,360,000、Indirect Cost: ¥708,000)
Fiscal Year 2011: ¥1,469,000 (Direct Cost: ¥1,130,000、Indirect Cost: ¥339,000)
Fiscal Year 2010: ¥1,599,000 (Direct Cost: ¥1,230,000、Indirect Cost: ¥369,000)
|
|---|
| Keywords | 骨細胞 / 骨芽細胞 / マイクロRNA / 次世代シークエンサー / 骨代謝 |
|---|
| Research Abstract |
Osteocyte is one of the most major cell in the bone, and dysfunction of osteocytes induce the osteoporosis. However, the physiological function and regulation mechanism of osteocyte are not completely known. Especially, there is no information about the mechanism of osteocyte differentiation. In this study, we focused on the microRNA, and examined the effect of microRNA in osteocyte differentiation. Firstly, we produced the osteocyte specific GFP expression mice. And, we isolated the osteocyte from these mice by using the FACS. The GFP positive cells expressed the marker gene of osteocyte higher than GFP negative cells, and osteoblast marker gene expression was lower. This result has been showed that GFP positive cell is osteocyte. We can use these mice and the osetocyte isolation method to analyze the expression of miRNA in osteocyte.
|
