The effect of microRNA in osteocyte differentiation.

• Project/Area Number: 22890178
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Orthopaedic surgery
• Research Institution: Keio University
• Principal Investigator: OCHI Hiroki 慶應義塾大学, 医学部, 特任助教 (30582283)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥3,068,000 (Direct Cost: ¥2,360,000、Indirect Cost: ¥708,000); Fiscal Year 2011: ¥1,469,000 (Direct Cost: ¥1,130,000、Indirect Cost: ¥339,000); Fiscal Year 2010: ¥1,599,000 (Direct Cost: ¥1,230,000、Indirect Cost: ¥369,000)
• Keywords: 骨細胞 / 骨芽細胞 / マイクロRNA / 次世代シークエンサー / 骨代謝

Research Abstract

Osteocyte is one of the most major cell in the bone, and dysfunction of osteocytes induce the osteoporosis. However, the physiological function and regulation mechanism of osteocyte are not completely known. Especially, there is no information about the mechanism of osteocyte differentiation. In this study, we focused on the microRNA, and examined the effect of microRNA in osteocyte differentiation. Firstly, we produced the osteocyte specific GFP expression mice. And, we isolated the osteocyte from these mice by using the FACS. The GFP positive cells expressed the marker gene of osteocyte higher than GFP negative cells, and osteoblast marker gene expression was lower. This result has been showed that GFP positive cell is osteocyte. We can use these mice and the osetocyte isolation method to analyze the expression of miRNA in osteocyte.

Research Products

• [Journal Article] Vitamin E decreases bone mass by stimulating osteoclast fusion (2012)
  • Author(s): Fujita K, Iwasaki M, Ochi H, Fukuda T, Ma C, Miyamoto T, Takitani K, Negishi-Koga T, Sunamura S, Kodama T, Takayanagi H, Tamai H, Kato S, Arai H, Shinomiya K, Itoh H, Okawa A, Takeda S
  • Journal Title: Nat Med Volume: 18(4) Pages: 589-94
  • Peer Reviewed
• [Journal Article] Positive regulation of osteoclastic differentiation by growth differentiation factor 15 upregulated in osteocytic cells under hypoxia (2012)
  • Author(s): Hinoi E, Ochi H, Takarada T, Nakatani E, Iezaki T, Nakajima H, Fujita H, Takahata Y, Hidano S, Kobayashi T, Takeda S, Yoneda Y
  • Journal Title: J Bone Miner Res Volume: 27(4) Pages: 938-49
  • Peer Reviewed
• [Journal Article] Vitamin E decreases bone mass by stimulating osteoclast fusion (2012)
  • Author(s): Fujita K, et al
  • Journal Title: Nat Med Volume: (In press) Issue: 4 Pages: 589-594
  • DOI: 10.1038/nm.2659
  • Peer Reviewed
• [Journal Article] Effects of long-term administration of carprofen on healing of a tibial osteotomy in dogs (2011)
  • Author(s): Ochi H, Hara Y, Asou Y, Harada Y, Nezu Y, Yogo T, Shinomiya K, Tagawa M
  • Journal Title: Am J Vet Res Volume: 72(5) Pages: 634-41
  • Peer Reviewed
• [Journal Article] Runx2 expression correlates with the degree of disc aging : a comparison between Magnetic Resonance Imaging and Runx2 expression
  • Author(s): Itoh H, Hara Y, Tagawa M, Kato T, Ochi H, Koga D, Okawa A, Shinomiya K, Asou Y
  • Journal Title: Am J Vet Res Volume: (in press)
  • Peer Reviewed
• [Presentation] ビタミンEは破骨細胞融合を促進し骨量減少を引き起こす (2011)
  • Author(s): 越智広樹
  • Organizer: 第13回骨粗鬆症学会骨ドック・検診分科会
  • Place of Presentation: 神戸
  • Year and Date: 2011-11-04
• [Book] Clinical Calcium ;特集骨・関節疾患の動物モデルI.骨粗鬆症8.骨粗鬆症研究のための遺伝子改変マウス (2011)
  • Author(s): 越智広樹、竹田秀
  • Publisher: 医薬ジャーナル社
• [Book] O. li. v. e.-骨代謝と生活習慣病の連関;腸由来セロトニン合成の薬理学的阻害が骨粗鬆症の骨同化治療に使用できる可能性 (2011)
  • Author(s): 越智広樹、竹田秀
  • Publisher: メディカルレビュー社