Elucidation of the role of monocarboxylate transporters on chondrocytes' degenerative change of joint desease

• Project/Area Number: 22890181
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Functional basic dentistry
• Research Institution: Showa University
• Principal Investigator: YOSHIMURA Kentaro 昭和大学, 歯学部, ポストドクター (10585699)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥2,977,000 (Direct Cost: ¥2,290,000、Indirect Cost: ¥687,000); Fiscal Year 2011: ¥1,417,000 (Direct Cost: ¥1,090,000、Indirect Cost: ¥327,000); Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 口腔生化学 / 軟骨変性疾患 / MCT-1 / IL-1β / 活性酸素種 / NOX-2 / 軟骨 / 遺伝子発現 / 炎症性疾患 / シグナル伝達

Research Abstract

Interleukin-1β(IL-1β) induces cell death in chondrocytes in a nitric oxide(NO)-and reactive oxygen species(ROS)-dependent manner. In this study, increased production of lactate was observed in IL-1β-treated mouse chondrocytic ATDC5 cells prior to the onset of their death. IL-1β-induced cell death in ATDC5 cells was suppressed by introducing an siRNA for monocarboxylate transporter-1(MCT-1), a lactate transporter distributed in plasma and mitochondrial inner membranes.

Research Products

• [Journal Article] Expression of POEM, a positive regulator of osteoblast differentiation, is suppressed by TNF-α (2011)
  • Author(s): Tsukasaki M, Yamada A, Suzuki D, Aizawa R, Miyazono A, Miyamoto Y, Suzawa T, Takami M, Yoshimura K, Morimura N, Yamamoto M, Kamijo R
  • Journal Title: Biochem Biophys Res Commun Volume: 410 Pages: 766-770
  • Peer Reviewed
• [Journal Article] Monocarboxylate transporter-1 is required for cell death in mouse chondrocytic ATDC5 cells exposed to interleukin-1. via activation of nuclear factor-. B and expression of phagocyte-type NADPH-oxidase (2011)
  • Author(s): Yoshimura K, Miyamoto Y, Yasuhara R, Maruyama T, Akiyama T, Yamada A, Takami M, Suzawa T, Tsunawaki S, Tachikawa T, Baba K, Kamijo R
  • Journal Title: J Biol Chem Volume: 286 Pages: 14744-14752
  • Peer Reviewed
• [Journal Article] Monocarboxylate transporter-1 is required for cell death in mouse chondrocytic ATDC5 cells exposed to interleukin-1β via activation of nuclear factor-κB and expression of phagocyte-type NADPH-oxidase (2011)
  • Author(s): Yoshimura K, et al
  • Journal Title: J Biol Chem Volume: 286 Issue: 17 Pages: 14744-14752
  • DOI: 10.1074/jbc.m111.221259
  • Peer Reviewed
• [Journal Article] MONOCARBOXYLATE TRANSPORTER-1 IS REQUIRED FOR CELL DEATH IN MOUSE CHONDROCYTIC ATDC5 CELLS EXPOSED TO INTERLEUKIN-1β VIA LATE PHASE ACTIVATION OF NUCLEAR FACTOR κB AND EXPRESSION OF PHAGOCYTE-TYPE NADPH OXIDASE (2011)
  • Author(s): Yoshimura K, et al.
  • Journal Title: The Journal of Biological Chemistry Volume: 286(印刷中)
  • Peer Reviewed
• [Presentation] TNF-.は細胞接着分子POEMの発現を抑制し、骨芽細胞分化を制御している (2011)
  • Author(s): 塚崎雅之, 他
  • Organizer: 第31回昭和歯学会総会
  • Place of Presentation: 東京
  • Year and Date: 2011-07-02
• [Presentation] TNF-αは細胞接着分子POEMの発現を抑制し、骨芽細胞分化を制御している (2011)
  • Author(s): 塚崎雅之, 他
  • Organizer: 第31回昭和歯学会総会
  • Place of Presentation: 東京
  • Year and Date: 2011-07-02
• [Presentation] Enhancement of inflammatory osteoclastogenesis by lysine-specific gingipain (2011)
  • Author(s): Akiyama T, et al
  • Organizer: IOF Regionals-2nd Asia-Pacific Osteoporosis and Bone Meeting and the Australian and New Zealand Bone and Mineral Society(ANZBMS) Annual Scientific Meeting with the Japanese Society for Bone and Mineral Society(JSBMR)
  • Place of Presentation: Gold Coast, Australia
• [Presentation] Monocarboxylate transporter-1は骨芽細胞分化を正に制御する (2011)
  • Author(s): 吉村健太郎, 他
  • Organizer: 第29回日本骨代謝学会学術集会
  • Place of Presentation: 大阪
• [Presentation] リシン特異的ジンジパインによる炎症性骨破壊促進機構の解析 (2011)
  • Author(s): 秋山智人, 他
  • Organizer: 第29回日本骨代謝学会学術集会
  • Place of Presentation: 大阪
• [Presentation] Monocarboxylate transporter-1 is required for cell death in mouse chondrocyte-kike ATDC5 cells exposed to interleukin-1. via late phase activation of NF-. B and expression of phagocyte-type NADPH-oxidase (2011)
  • Author(s): Yoshimura K, et al
  • Organizer: The Joint International Meeting Sponsored by The Japanese Society for Interferon and Cytokine Research, The Japanese Society for Macrophage Molecular and Cell Biology, Cytokine/Chemokine, Immunity, and Diseases
  • Place of Presentation: Osaka, Japan
• [Presentation] Dual effects of lysine-specific gingipain on inflammatory osteoclastogenesis (2011)
  • Author(s): Akiyama T, et al
  • Organizer: The Joint International Meeting Sponsored by The Japanese Society for Interferon and Cytokine Research, The Japanese Society for Macrophage Molecular and Cell Biology, Cytokine/Chemokine, Immunity, and Diseases
  • Place of Presentation: Osaka, Japan
• [Presentation] Dual effect of lysine-specific gingipain on inflammatory osteoclastgenesis (2011)
  • Author(s): Akiyama T, et al
  • Organizer: The Joint International Meeting Sponsored by The Japanese Society for Interferon and Cytekine Research, The Japanese Society for Macrophage Molecullar and Cell Biology, Cytokine/Chemokine, Immunity, and Disease
  • Place of Presentation: Osaka, Japan
• [Presentation] Monocarboxylate transporter-1 is requiredfor cell death in mouse chondrocyte-like ATDC5 cells exposed to interleukin-1β via late-phase activation of NF-κB and expression of phagocyte-type NADPH-oxidase (2011)
  • Author(s): Yoshimura K, et al
  • Organizer: The Joint International Meeting Sponsored by The Japanese Society for Interferon and Cytekine Research, The Japanese Society for Macrophage Molecullar and Cell Biology, Cytokine/Chemokine, Immunity, and Disease
  • Place of Presentation: Osaka, Japan
• [Presentation] Enhancement of inflammatory osteoclastgenesis by lysine-specific gingipain (2011)
  • Author(s): Akiyama T, et al
  • Organizer: IOF Regionals-2nd Asia-Pacific Osteoporosis and Bone Meeting and the Australian and New Zealand Bone and Mineral Society (ANZBMS) Annual Scientific Meeting with the Japanese Society for Bone and Mineral Society (JSBMR)
  • Place of Presentation: Gold Coast, Australia
• [Presentation] Monocarboxylate transporter-1 is required for IL-1β-induced cell death in chondrocytes (2011)
  • Author(s): Yoshimura K
  • Organizer: 89th General Session & Exhibition of the International Association for Dental Research
  • Place of Presentation: San Diego, CA, USA
• [Presentation] Carbonic anhydrase IX suppresses hypertrophic differentiation of chondrocytes (2010)
  • Author(s): Maruyama T, et al
  • Organizer: The 32nd Annual Meeting of American Society of Bone and Mineral Research
  • Place of Presentation: Toronto, Canada
• [Presentation] Monocarboxylate transporter-1は、IL-1β刺激後の軟骨細胞で後期NF-κB活性化を起こし、NOX-2発現とそれに依存した細胞死を誘導する (2010)
  • Author(s): 吉村健太郎, 他
  • Organizer: 第52回歯科基礎医学会学術大会
  • Place of Presentation: 東京
• [Presentation] Monocarboxylate transporter-1は、IL-1β刺激後の軟骨細胞で後期NF-κB活性化を起こし、NOX-2発現とそれに依存した細胞死を誘導する (2010)
  • Author(s): 吉村健太郎, 他
  • Organizer: 第28回日本骨代謝学会学術集会
  • Place of Presentation: 東京
• [Presentation] Monocarboxylate transporter-1 is involved in activation of nuclear factor-κB and expression of phogocyte-type NADPH-oxidase in mouse chondrocytic ATDC5 cells exposed to interleukin-1β (2010)
  • Author(s): Yoshimura K, et al
  • Organizer: The 6th International Conference on the Biology, Chemistry, and Therapeutic Application of Nitric Oxide
  • Place of Presentation: Kyoto, Japan
• [Presentation] Nitric oxide promotes odontoblastic differentiation (2010)
  • Author(s): Miyamoto Y, et al
  • Organizer: The 6th International Conference on the Biology, Chemistry, and Therapeutic Application of Nitric Oxide
  • Place of Presentation: Kyoto, Japan
• [Presentation] Monocarboxylate transporter-1は、IL-1β刺激後の軟骨細胞で後期NF-κB活性化を起こし、NOX-2発現とそれに依存した細胞死を誘導する (2010)
  • Author(s): 吉村健太郎
  • Organizer: 第52回歯科基礎医学会学術集会
  • Place of Presentation: 東京
• [Presentation] monocarboxylate transporter-1 is involved in activation of nuclear factor-κB and expression of phagocyte-type NADPH-oxidase in mouse chondrocytic ATDC5 cells exposed to interleukin-1β (2010)
  • Author(s): Yoshimura K
  • Organizer: The 6th International Conference on the Biology, Chemistry, and Therapeutic Application of Nitric Oxide
  • Place of Presentation: Kyoto, Japan
• [Remarks]
• [Remarks]
  • URL: http://www10.showa-u.ac.jp/~oralbio/
• [Remarks]
  • URL: http://www10.showa-u.ac.jp/~oralbio/