The study on epithelial cells-mediated development of allergic diseases

• Project/Area Number: 22890224
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: 膠原病・アレルギー・感染症内科学
• Research Institution: Hyogo Medical University
• Principal Investigator: MATSUSHITA Kazufumi 兵庫医科大学, 医学部, 助教 (20581549)
• Research Collaborator: YOSHIMOTO Tomohiro 兵庫医科大学, 医学部, 教授 (60241171)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥3,068,000 (Direct Cost: ¥2,360,000、Indirect Cost: ¥708,000); Fiscal Year 2011: ¥1,469,000 (Direct Cost: ¥1,130,000、Indirect Cost: ¥339,000); Fiscal Year 2010: ¥1,599,000 (Direct Cost: ¥1,230,000、Indirect Cost: ¥369,000)
• Keywords: アレルギー / ぜんそく / アレルギー・ぜんそく / 免疫学

Research Abstract

IL-33 is a newly identified IL-1 family cytokine, which stimulates basophile, mast cell, dendritic cell or T cell to augment Th2 response and exacerbate allergic diseases. Recent studies have revealed not only cleaved, so called"mature-form", but also full length, so called"pre-form", IL-33 has its biological activity suggesting the importance of IL-33 up-regulation itself in the initiation or exacerbation of allergic diseases. However, the mechanisms for IL-33 induction are yet to be fully uncovered. Here, we studied the regulatory mechanisms of IL-33 protein and mRNA expression in Ragweed pollen induced allergic rhinitis and asthma mouse model. Further more, to gain insight into the precise regulatory mechanisms for IL-33 mRNA expression in the epithelial cells, we investigated Ragweed pollen and PMA and ionomycin induced IL-33 mRNA expression in human bronchial epithelial cell line, BEAS-2B.

Research Products

• [Journal Article] A critical role of IL-33 in experimental allergic rhinitis (2012)
  • Author(s): Haenuki Y, Matsushita K, Futatsugi-Yumikura S, Ishii KJ, Kawagoe T, Imoto Y, Fujieda S, Yasuda M, Hisa Y, Akira S, Nakanishi K, Yoshimoto T.
  • Journal Title: J. Allergy Clin. Immunol Volume: (in press)
  • Peer Reviewed
• [Journal Article] Contribution of IL-33-activated type II innate lymphoid cells to pulmonary eosinophilia in intestinal nematode-infected mice (2012)
  • Author(s): Yasuda K, Muto T, Kawagoe T, Matsumoto M, Sasaki Y, Matsushita K, Taki Y, Futatsugi-Yumikura S, Tsutsui H, Ishii KJ, Yoshimoto T, Akira S, Nakanishi K.
  • Journal Title: Proc. Natl. Acad. Sci. U. S. A Volume: 109 Pages: 3451-3456
  • Peer Reviewed
• [Journal Article] A critical role of IL-33 in experimental allergic rhinitis (2012)
  • Author(s): Haenuki Y, Matsushita K, Futatsugi-Yumikura S, Ishii KJ, Kawagoe T, Imoto Y, Fujieda S, Yasuda M, Hisa Y, Akira S, Nakanishi K, Yoshimoto T
  • Journal Title: J Allergy Clin Immunol Volume: (in press) Issue: 1 Pages: 184-194
  • DOI: 10.1016/j.jaci.2012.02.013
  • Peer Reviewed
• [Journal Article] Contribution of IL-33-activated type II innate lymphoid cells to pulmonary eosinophilia in intestinal nematode-infected mice (2012)
  • Author(s): 安田好文, 他
  • Journal Title: Proceedings of the National Academy of Sciences of the United States of America Volume: 109 Issue: 9 Pages: 3451-3456
  • DOI: 10.1073/pnas.1201042109
  • Peer Reviewed
• [Journal Article] The IkB kinase complex regulates TLR-IL-1R induced cytokine mRNA stability by controlling Regnase-1 degradation (2011)
  • Author(s): Iwasaki H, Takeuchi O, Teraguchi S, Matsushita K, Uehata T, Kuniyoshi K, Satoh T, Saitoh T, Matsushita M, Standley DM and Akira S.
  • Journal Title: Nat. Immunol Volume: 12 Pages: 1167-1175
  • Peer Reviewed
• [Journal Article] The IκB kinase complex regulates the stability of cytokine-encoding mRNA induced by TLR-IL-1R by controlling degradation of regnase-1. (2011)
  • Author(s): Iwasaki H, Takeuchi O, Teraguchi S, Matsushita K, Uehata T, Kuniyoshi K, Satoh T, Saitoh T, Matsushita M, Standley DM, Akira S.
  • Journal Title: Nat Immunol. Volume: 12 Issue: 12 Pages: 1167-1175
  • DOI: 10.1038/ni.2137
  • Peer Reviewed
• [Journal Article] IkappaBzeta is essential for natural killer cell activation in response to IL-12 and IL-18 (2010)
  • Author(s): Miyake T, Satoh T, Kato H, Matsushita K, Kumagai Y, Vandenbon A, Tani T, Muta T, Akira S and Takeuchi O.
  • Journal Title: Proc. Natl. Acad. Sci. U. S. A Volume: 107 Pages: 17680-17685
  • Peer Reviewed
• [Journal Article] The Jmjd3-Irf4 axis regulates M2 macrophage polarization and host responses against helminth infection (2010)
  • Author(s): Satoh T, Takeuchi O, Vandenbon A, Yasuda K, Tanaka Y, Kumagai Y, Miyake T, Matsushita K, Okazaki T, Saitoh T, Honma K, Matsuyama T, Yui K, Tsujimura T, Standley DM, Nakanishi K, Nakai K and Akira S.
  • Journal Title: Nat. Immunol Volume: 11 Pages: 926-44
  • Peer Reviewed
• [Journal Article] LGP2 is a positive regulator of RIG-I-and MDA5-mediated antiviral responses (2010)
  • Author(s): Satoh T, Kato H, Kumagai Y, Yoneyama M, Sato S, Matsushita K, Tsujimura T, Fujita T, Akira S and Takeuchi O.
  • Journal Title: Proc Natl Acad Sci U. S. A Volume: 107 Pages: 1512-1517
  • Peer Reviewed
• [Journal Article] IkappaBzeta is essential for natural killer cell activation in response to IL-12 and IL-18. (2010)
  • Author(s): Miyake T, Satoh T, Kato H, Matsushita K, Kumagai Y, Vandenbon A, Tani T, Muta T, Akira S, Takeuchi O.
  • Journal Title: Proc Natl Acad Sci U.S.A. Volume: 107 Pages: 17680-17685
  • Peer Reviewed
• [Journal Article] The Jmjd3-Irf4 axis regulates M2 macrophage polarization and host responses against helminth infection. (2010)
  • Author(s): Satoh T, Takeuchi O, Vandenbon A, Yasuda K, Tanaka Y, Kumagai Y, Miyake T, Matsushita K, Okazaki T, Saitoh T, Honma K, Matsuyama T, Yui K, Tsujimura T, Standley DM, Nakanishi K, Nakai K, Akira S.
  • Journal Title: Nature Immunology Volume: 11 Pages: 926-944
  • Peer Reviewed
• [Presentation] A novel TLR-inducible RNase Zc3h12a mediated regulation of T and B cell activation (2010)
  • Author(s): Matsushita K, Takeuchi O, Iwasaki H, Akira S.
  • Organizer: International Congress of Immunology
  • Place of Presentation: Kobe, Japan
  • Year and Date: 2010-08-25
• [Presentation] A novel TLR-inducible RNase Zc3h12a mediated regulation of T and B cell activation. (2010)
  • Author(s): Matsushita K, Takeuchi O, Iwasaki H, Akira S.
  • Organizer: International Congress of Immunology.
  • Place of Presentation: Kobe, Japan
  • Year and Date: 2010-08-25